使用光敏剂氯铝酞菁的低剂量光动力疗法对皮肤成纤维细胞划痕伤口模型的影响。

IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS
Efstathios Giannakopoulos, Annita Katopodi, Michail Rallis, Konstantinos Politopoulos, Eleni Alexandratou
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引用次数: 0

摘要

多年来,人们开发了不同的伤口愈合方法,但这些方法成本高昂,且对患者有不良影响。本文旨在体外成纤维细胞伤口愈合模型中研究低剂量光导光疗这种新型愈合方法。氯化铝酞菁(AlClPc)被用作光敏剂,由波长为 661 纳米的红色二极管激光器激活。优化光导疗法后,通过图像处理和分析对伤口闭合率和活性氧进行量化。结果显示,PDT 治疗组的伤口愈合率明显高于对照组。此外,研究还发现,长时间的活性氧增加并不会促进伤口闭合,而少量的活性氧增加则会触发伤口闭合,从而加快伤口闭合。总之,使用 AlClPc 的低剂量光紫外疗法能以依赖 ROS 的方式促进体外伤口愈合,因此可以推测在体内也会产生类似的积极效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The effect of low-dose photodynamic therapy using the photosensitizer chloroaluminum phthalocyanine on a scratch wound model in skin fibroblasts

The effect of low-dose photodynamic therapy using the photosensitizer chloroaluminum phthalocyanine on a scratch wound model in skin fibroblasts

Different approaches on wound healing have been developed over the years but they suffer from high costs and adverse effects for the patients. The current paper was designed to study low dose PDT, a novel healing approach, in an in vitro fibroblasts wound healing model. Chloroaluminum phthalocyanine (AlClPc) was used as photosensitizer and was activated by a red diode laser at 661 nm. After PDT optimization, wound closure rate and reactive oxygen species were quantified by image processing and analysis. Our results revealed that wound healing rates were significantly higher in PDT treated groups than in the control. Additionally, the study revealed that a prolonged ROS increase did not promote wound closure, while a small increase acted as a trigger, resulting in faster wound closure. Concluding, low dose PDT using AlClPc enhances wound healing in vitro in a ROS dependent manner, allowing the assumption of similar positive effects in vivo.

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来源期刊
Journal of Biophotonics
Journal of Biophotonics 生物-生化研究方法
CiteScore
5.70
自引率
7.10%
发文量
248
审稿时长
1 months
期刊介绍: The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.
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