Galectin-1 通过 NRP-1/c-JUN/Wee1 通路促进胃癌进展和顺铂抗性

IF 3.2 4区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY
Zhengyang Pan, Guoxi Xu, Yan Zhang, Meiling Wu, Jiahui Yu, Xujun He, Wei Zhang, Junfeng Hu
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引用次数: 0

摘要

目的:胃癌(GC)是最致命的恶性肿瘤之一,也是全球癌症相关死亡的第三大原因。Galectin-1(Gal-1)是癌症相关成纤维细胞(CAFs)分泌的一种主要蛋白质;然而,Gal-1在胃癌中的作用和作用机制仍不清楚。在本研究中,我们用外源性人重组 galectin-1 蛋白(rhGal-1)刺激 GC 细胞,研究其对细胞增殖、迁移和顺铂抗性的影响:我们利用模拟的rhGal-1蛋白作为CAFs产生的旁分泌因子诱导GC细胞,并研究其在GC进展和顺铂抗性中的促进作用和机制。免疫组化(IHC)检测证实,Gal-1的表达与临床病理参数相关,并与神经蛋白-1(NRP-1)、c-JUN和Wee1的表达相关:结果:我们的研究显示,Gal-1的表达与不良预后显著相关。Gal-1通过激活NRP-1/C-JUN/Wee1通路促进GC细胞的增殖和转移。NRP-1抑制剂EG00229能有效抵消这些影响:这些发现揭示了 Gal-1 促进 GC 生长并导致化疗耐药性的潜在机制,为治疗 GC 提供了新的治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Galectin-1 Promotes Gastric Carcinoma Progression and Cisplatin Resistance Through the NRP-1/c-JUN/Wee1 Pathway.

Purpose: Gastric cancer (GC) is among the deadliest malignancies and the third leading cause of cancer-related deaths worldwide. Galectin-1 (Gal-1) is a primary protein secreted by cancer-associated fibroblasts (CAFs); however, its role and mechanisms of action of Gal-1 in GC remain unclear. In this study, we stimulated GC cells with exogenous human recombinant galectin-1 protein (rhGal-1) to investigate its effects on the proliferation, migration, and resistance to cisplatin.

Materials and methods: We used simulated rhGal-1 protein as a paracrine factor produced by CAFs to induce GC cells and investigated its promotional effects and mechanisms in GC progression and cisplatin resistance. Immunohistochemical (IHC) assay confirmed that Gal-1 expression was associated with clinicopathological parameters and correlated with the expression of neuropilin-1 (NRP-1), c-JUN, and Wee1.

Results: Our study reveals Gal-1 expression was significantly associated with poor outcomes. Gal-1 boosts the proliferation and metastasis of GC cells by activating the NRP-1/C-JUN/Wee1 pathway. Gal-1 notably increases GC cell resistance to cisplatin The NRP-1 inhibitor, EG00229, effectively counteracts these effects.

Conclusions: These findings revealed a potential mechanism by which Gal-1 promotes GC growth and contributes to chemoresistance, offering new therapeutic targets for the treatment of GC.

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来源期刊
Journal of Gastric Cancer
Journal of Gastric Cancer Biochemistry, Genetics and Molecular Biology-Cancer Research
CiteScore
4.30
自引率
12.00%
发文量
36
期刊介绍: The Journal of Gastric Cancer (J Gastric Cancer) is an international peer-reviewed journal. Each issue carries high quality clinical and translational researches on gastric neoplasms. Editorial Board of J Gastric Cancer publishes original articles on pathophysiology, molecular oncology, diagnosis, treatment, and prevention of gastric cancer as well as articles on dietary control and improving the quality of life for gastric cancer patients. J Gastric Cancer includes case reports, review articles, how I do it articles, editorials, and letters to the editor.
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