利用黑头鲦鱼 EcoToxChip 研究新型雌激素全氟乙醇（1H,1H,8H,8H-Perfluorooctane-1,8-diol，FC8-diol）的影响。

IF 3.6 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental Toxicology and Chemistry Pub Date : 2024-07-03 DOI:10.1002/etc.5937

Kelvin J Santana Rodriguez, Daniel L Villeneuve, Jenna E Cavallin, Brett R Blackwell, John Hoang, Rachel N Hofer, Kathleen M Jensen, Michael D Kahl, Robin N Kutsi, Emma Stacy, Mackenzie L Morshead, Gerald T Ankley

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引用次数: 0

摘要

在之前的一项体内研究中，成年雄性黑头呆鱼（Pimephales promelas）通过水接触 1H,1H,8H,8H-全氟辛烷-1,8-二醇（FC8-diol）4 天。本研究通过分析该研究中的 26 个雄性黑头鲦鱼肝脏 RNA 样本（每个测试浓度 5 个：0、0.018、0.051、0.171 和 0.463 毫克 FC8-二醇/升）。EcoToxChips 是一种定量聚合酶链式反应阵列，可同时测定 375 个物种的特异性毒理学基因。利用在线工具 EcoToxXplorer 对数据进行了分析。在分析的基因中，分别有 62 和 96 个基因在一种或多种 FC8-二醇处理下明显上调和下调。先前研究的基因表达结果得到了验证，显示卵黄素 mRNA（vtg）上调，胰岛素样生长因子 1 mRNA（igf1）下调。与雌激素受体激活有关的其他基因，包括 esr2a（雌激素受体 2a）和 esrrb（雌激素相关受体 beta）也受到了影响，这进一步证实了 FC8-二醇的雌激素性质。此外，与脂质和碳水化合物代谢、先天免疫反应、内分泌生殖和内分泌甲状腺有关的生物通路中的基因也受到了显著影响。这些结果既增强了人们使用生态毒理芯片工具推断化学作用模式的信心，又进一步揭示了 FC8-二醇可能产生的生物效应。环境毒物化学 2024;00:1-9。© 2024 SETAC.本文由美国政府雇员撰写，其作品在美国属于公共领域。

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Examining effects of a novel estrogenic perfluoro-alcohol, 1H,1H,8H,8H-Perfluorooctane-1,8-diol (FC8-diol), using the fathead minnow EcoToxChip.

In a previous in vivo study, adult male fathead minnows (Pimephales promelas) were exposed via water for 4 days to 1H,1H,8H,8H-perfluorooctane-1,8-diol (FC8-diol). The present study expands on the evaluation of molecular responses to this perfluoro-alcohol by analyzing 26 male fathead minnow liver RNA samples from that study (five from each test concentration: 0, 0.018, 0.051, 0.171, and 0.463 mg FC8-diol/L) using fathead minnow EcoToxChips Ver. 1.0. EcoToxChips are a quantitative polymerase chain reaction array that allows for simultaneous measurement of >375 species-specific genes of toxicological interest. Data were analyzed with the online tool EcoToxXplorer. Among the genes analyzed, 62 and 96 were significantly up- and downregulated, respectively, by one or more FC8-diol treatments. Gene expression results from the previous study were validated, showing an upregulation of vitellogenin mRNA (vtg) and downregulation of insulin-like growth factor 1 mRNA (igf1). Additional genes related to estrogen receptor activation including esr2a (estrogen receptor 2a) and esrrb (estrogen related receptor beta) were also affected, providing further confirmation of the estrogenic nature of FC8-diol. Furthermore, genes involved in biological pathways related to lipid and carbohydrate metabolism, innate immune response, endocrine reproduction, and endocrine thyroid were significantly affected. These results both add confidence in the use of the EcoToxChip tool for inferring chemical mode(s) of action and provide further insights into the possible biological effects of FC8-diol. Environ Toxicol Chem 2024;00:1-9. © 2024 SETAC. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.

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来源期刊

Environmental Toxicology and Chemistry 环境科学-毒理学

CiteScore

7.40

自引率

9.80%

发文量

265

审稿时长

3.4 months

期刊介绍： The Society of Environmental Toxicology and Chemistry (SETAC) publishes two journals: Environmental Toxicology and Chemistry (ET&C) and Integrated Environmental Assessment and Management (IEAM). Environmental Toxicology and Chemistry is dedicated to furthering scientific knowledge and disseminating information on environmental toxicology and chemistry, including the application of these sciences to risk assessment.[...] Environmental Toxicology and Chemistry is interdisciplinary in scope and integrates the fields of environmental toxicology; environmental, analytical, and molecular chemistry; ecology; physiology; biochemistry; microbiology; genetics; genomics; environmental engineering; chemical, environmental, and biological modeling; epidemiology; and earth sciences. ET&C seeks to publish papers describing original experimental or theoretical work that significantly advances understanding in the area of environmental toxicology, environmental chemistry and hazard/risk assessment. Emphasis is given to papers that enhance capabilities for the prediction, measurement, and assessment of the fate and effects of chemicals in the environment, rather than simply providing additional data. The scientific impact of papers is judged in terms of the breadth and depth of the findings and the expected influence on existing or future scientific practice. Methodological papers must make clear not only how the work differs from existing practice, but the significance of these differences to the field. Site-based research or monitoring must have regional or global implications beyond the particular site, such as evaluating processes, mechanisms, or theory under a natural environmental setting.