Temperature, water travel time, and dissolved organic matter structure river microbial communities in a large temperate watershed

There is growing evidence that the composition of river microbial communities gradually transitions from terrestrial taxa in headwaters to unique planktonic and biofilm taxa downstream. Yet, little is known about fundamental controls on this community transition across scales in river networks. We hypothesized that community composition is controlled by flow-weighted travel time of water, in combination with temperature and dissolved organic matter (DOM), via similar mechanisms postulated in the Pulse-Shunt Concept for DOM. Bacterioplankton and biofilm samples were collected at least quarterly for 2 yr at 30 sites throughout the Connecticut River watershed. Among hydrologic variables, travel time was a better predictor of both bacterioplankton and biofilm community structure than watershed area, dendritic distance, or discharge. Among all variables, both bacterioplankton and biofilm composition correlated with travel time, temperature, and DOM composition. Bacterioplankton beta-diversity was highest at shorter travel times (< 1 d) and decreased with increasing travel time, showing progressive homogenization as water flows downstream. Bacterioplankton and biofilm communities were similar at short travel times, but diverged as travel time increased. Bacterioplankton composition at downstream sites more closely resembled headwater communities when temperatures were cooler and travel times shorter. These findings suggest that the pace and trajectory of riverine bacterioplankton community succession may be controlled by temperature-regulated growth rate and time for communities to grow and change. Moreover, bacterioplankton, and to a lesser extent biofilm, may experience the same hydrologic forcing hypothesized in the Pulse-Shunt Concept for DOM, suggesting that hydrology controls the dispersal of microbial communities in river networks.