金黄色葡萄球菌的 DUF3055 采用独特策略实现结构独特性

IF 2.3 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Hyo Jung Kim, Hyoun Sook Kim
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引用次数: 0

摘要

金黄色葡萄球菌仍然是一种公共卫生威胁,世卫组织将这种病原体列为开发新型抗菌剂的重点。全基因组测序发现了许多对细胞存活和感染至关重要的保守基因。对这些蛋白质的结构和功能进行鉴定势必会有助于新抗菌药的开发。因此,本研究阐明了来自金黄色葡萄球菌污点 Mu50 的假定蛋白 DUF3055 的结构。该蛋白具有尚未明确的功能和独特的折叠。DUF3055 的大小使其成为核磁共振表征的理想候选对象,核磁共振与圆二色性结合发现该蛋白质是折叠的。结晶和结构解析发现,整个二聚体折叠具有一个由β凸起和紧密交叉的α螺旋形成的带负电的表面,其大小与DNA单匝互补。我们的结构观察结果表明,金黄色葡萄球菌的假定蛋白DUF3055在DNA结合和基因调控中发挥作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
DUF3055 from Staphylococcus aureus adopts unique strategy for structural distinctiveness

Staphylococcus aureus remains a public health threat with the WHO classifying the pathogen as a high priority in the development of new antimicrobial agents. Whole genome sequencing has revealed a number of conserved genes that may be essential for cell viability and infection. Characterising the structure and function of these proteins will inevitably aid development of new antimicrobials. Therefore, this study elucidated the structure of hypothetical protein DUF3055 from S. aureus stain Mu50. The protein possesses an as yet undefined function and a unique fold. The size of DUF3055 made it an ideal candidate for NMR characterisation which in conjunction with circular dichroism revealed the protein to be folded. Crystallisation and structural solution found that the overall dimer fold has a negatively charged surface formed by a β-bulge and tightly crossed α-helices, with a complementary size to a DNA single turn. Our structural observations suggest that hypothetical protein DUF3055 from S. aureus has a role in DNA binding and gene regulation.

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来源期刊
Applied Biological Chemistry
Applied Biological Chemistry Chemistry-Organic Chemistry
CiteScore
5.40
自引率
6.20%
发文量
70
审稿时长
20 weeks
期刊介绍: Applied Biological Chemistry aims to promote the interchange and dissemination of scientific data among researchers in the field of agricultural and biological chemistry. The journal covers biochemistry and molecular biology, medical and biomaterial science, food science, and environmental science as applied to multidisciplinary agriculture.
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