Bumkyu Kim , Jose M. Perez , Steven D. Karlen , Jason Coplien , Timothy J. Donohue , Daniel R. Noguera
{"title":"利用芳香酵母菌(Novosphingobium aromaticivorans†)从芳香水流中获得高产能的 2-吡喃酮-4,6-二羧酸","authors":"Bumkyu Kim , Jose M. Perez , Steven D. Karlen , Jason Coplien , Timothy J. Donohue , Daniel R. Noguera","doi":"10.1039/d4gc01975j","DOIUrl":null,"url":null,"abstract":"<div><p>Enhancing the production of biochemicals from lignocellulosic biomass is one potential way to decrease society's dependence on fossil fuels. Aromatic compounds obtained from plant biomass can be used as substrates for microbial production of dicarboxylic acids such as 2-pyrone-4,6-dicarboxylic acid (PDC) and <em>cis</em>,<em>cis</em>-muconic acid, which are building blocks for the manufacturing of polymer-based fibers and materials. In this study, we used an engineered strain of the bacterium <em>Novosphingobium aromaticivorans</em> to investigate how to increase PDC productivity in flow-through bioreactors receiving aqueous solutions of aromatics. At the best operational conditions tested, we achieved stable PDC production rates of 0.77 g<sub>PDC</sub> L<sup>−1</sup> h<sup>−1</sup> with <em>p</em>-hydroxybenzoic acid, 1.93 g<sub>PDC</sub> L<sup>−1</sup> h<sup>−1</sup> with syringic acid, and 1.53 g<sub>PDC</sub> L<sup>−1</sup> h<sup>−1</sup> with the products from alkaline pretreated poplar biomass. PDC titers in these reactors ranged from 7.7 to 15 g L<sup>−1</sup> (42 to 80 mM) and were limited by aromatic solubility in the case of syringic acid, or by accumulation of protocatechuic acid from <em>p</em>-hydroxybenzoic acid when high aromatic loading rates were used. The use of high aromatic loading rates, hollow-fiber membranes to concentrate the microbial cells, and NH<sub>4</sub>OH for pH control were factors that contributed to this study achieving the highest PDC productivities reported to date. Overall, our findings demonstrate strategies that can be used to increase bioreactor productivity when aromatic substrates are delivered in aqueous form. These findings may also provide useful insight for production of other biochemicals from aromatic streams using <em>N. aromaticivorans</em> or other microbial chassis.</p></div>","PeriodicalId":78,"journal":{"name":"Green Chemistry","volume":null,"pages":null},"PeriodicalIF":9.3000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/gc/d4gc01975j?page=search","citationCount":"0","resultStr":"{\"title\":\"Achieving high productivity of 2-pyrone-4,6-dicarboxylic acid from aqueous aromatic streams with Novosphingobium aromaticivorans†\",\"authors\":\"Bumkyu Kim , Jose M. Perez , Steven D. Karlen , Jason Coplien , Timothy J. Donohue , Daniel R. Noguera\",\"doi\":\"10.1039/d4gc01975j\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Enhancing the production of biochemicals from lignocellulosic biomass is one potential way to decrease society's dependence on fossil fuels. Aromatic compounds obtained from plant biomass can be used as substrates for microbial production of dicarboxylic acids such as 2-pyrone-4,6-dicarboxylic acid (PDC) and <em>cis</em>,<em>cis</em>-muconic acid, which are building blocks for the manufacturing of polymer-based fibers and materials. In this study, we used an engineered strain of the bacterium <em>Novosphingobium aromaticivorans</em> to investigate how to increase PDC productivity in flow-through bioreactors receiving aqueous solutions of aromatics. At the best operational conditions tested, we achieved stable PDC production rates of 0.77 g<sub>PDC</sub> L<sup>−1</sup> h<sup>−1</sup> with <em>p</em>-hydroxybenzoic acid, 1.93 g<sub>PDC</sub> L<sup>−1</sup> h<sup>−1</sup> with syringic acid, and 1.53 g<sub>PDC</sub> L<sup>−1</sup> h<sup>−1</sup> with the products from alkaline pretreated poplar biomass. PDC titers in these reactors ranged from 7.7 to 15 g L<sup>−1</sup> (42 to 80 mM) and were limited by aromatic solubility in the case of syringic acid, or by accumulation of protocatechuic acid from <em>p</em>-hydroxybenzoic acid when high aromatic loading rates were used. The use of high aromatic loading rates, hollow-fiber membranes to concentrate the microbial cells, and NH<sub>4</sub>OH for pH control were factors that contributed to this study achieving the highest PDC productivities reported to date. Overall, our findings demonstrate strategies that can be used to increase bioreactor productivity when aromatic substrates are delivered in aqueous form. 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Achieving high productivity of 2-pyrone-4,6-dicarboxylic acid from aqueous aromatic streams with Novosphingobium aromaticivorans†
Enhancing the production of biochemicals from lignocellulosic biomass is one potential way to decrease society's dependence on fossil fuels. Aromatic compounds obtained from plant biomass can be used as substrates for microbial production of dicarboxylic acids such as 2-pyrone-4,6-dicarboxylic acid (PDC) and cis,cis-muconic acid, which are building blocks for the manufacturing of polymer-based fibers and materials. In this study, we used an engineered strain of the bacterium Novosphingobium aromaticivorans to investigate how to increase PDC productivity in flow-through bioreactors receiving aqueous solutions of aromatics. At the best operational conditions tested, we achieved stable PDC production rates of 0.77 gPDC L−1 h−1 with p-hydroxybenzoic acid, 1.93 gPDC L−1 h−1 with syringic acid, and 1.53 gPDC L−1 h−1 with the products from alkaline pretreated poplar biomass. PDC titers in these reactors ranged from 7.7 to 15 g L−1 (42 to 80 mM) and were limited by aromatic solubility in the case of syringic acid, or by accumulation of protocatechuic acid from p-hydroxybenzoic acid when high aromatic loading rates were used. The use of high aromatic loading rates, hollow-fiber membranes to concentrate the microbial cells, and NH4OH for pH control were factors that contributed to this study achieving the highest PDC productivities reported to date. Overall, our findings demonstrate strategies that can be used to increase bioreactor productivity when aromatic substrates are delivered in aqueous form. These findings may also provide useful insight for production of other biochemicals from aromatic streams using N. aromaticivorans or other microbial chassis.
期刊介绍:
Green Chemistry is a journal that provides a unique forum for the publication of innovative research on the development of alternative green and sustainable technologies. The scope of Green Chemistry is based on the definition proposed by Anastas and Warner (Green Chemistry: Theory and Practice, P T Anastas and J C Warner, Oxford University Press, Oxford, 1998), which defines green chemistry as the utilisation of a set of principles that reduces or eliminates the use or generation of hazardous substances in the design, manufacture and application of chemical products. Green Chemistry aims to reduce the environmental impact of the chemical enterprise by developing a technology base that is inherently non-toxic to living things and the environment. The journal welcomes submissions on all aspects of research relating to this endeavor and publishes original and significant cutting-edge research that is likely to be of wide general appeal. For a work to be published, it must present a significant advance in green chemistry, including a comparison with existing methods and a demonstration of advantages over those methods.