Christina Karakosta, Martina Samiotaki, George Panayotou, Dimitrios Papakonstantinou, Marilita M. Moschos
{"title":"肌动蛋白结合蛋白在白内障形成中的作用","authors":"Christina Karakosta, Martina Samiotaki, George Panayotou, Dimitrios Papakonstantinou, Marilita M. Moschos","doi":"10.1002/cm.21889","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Introduction</h3>\n \n <p>Actin has been implicated in lens opacification; however, the specific actin-related pathways involved in cataracts remain unelucidated. In this study, actin-related proteome changes and signaling pathways involved in the development of cataracts were evaluated.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>The anterior capsule and phacoemulsification (phaco) cassette contents were collected during cataract surgery from 11 patients with diabetic cataract (DC), 12 patients with age-related cataract (ARC), and seven patients with post-vitrectomy cataract (PVC). Untargeted, global identification and quantification of proteins was performed through liquid chromatography-mass spectrometry with the data-independent acquisition (DIA).</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>In phaco cassette samples, proteins with significantly lower expression in ARC than in DC and PVC were involved in various pathways, including actin binding, actin cytoskeleton reorganization, actin filament capping, cortical actin cytoskeleton organization, and small GTPase-mediated signal transduction pathways. In anterior capsules, proteins with significantly lower expression in ARC than in DC and PVC were involved in actin binding and actin cytoskeleton reorganization pathways.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Actin cytoskeleton and actin-binding proteins are involved in lens fiber elongation and differentiation. Rho GTPases contribute to actin cytoskeletal reorganization, and their inactivation is linked to abnormal lens fiber migration. These findings link actin binding to lens fiber integrity, lens opacification, and cataracts.</p>\n </section>\n </div>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"82 3","pages":"98-110"},"PeriodicalIF":2.4000,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21889","citationCount":"0","resultStr":"{\"title\":\"Role of actin-binding proteins in cataract formation\",\"authors\":\"Christina Karakosta, Martina Samiotaki, George Panayotou, Dimitrios Papakonstantinou, Marilita M. 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Untargeted, global identification and quantification of proteins was performed through liquid chromatography-mass spectrometry with the data-independent acquisition (DIA).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>In phaco cassette samples, proteins with significantly lower expression in ARC than in DC and PVC were involved in various pathways, including actin binding, actin cytoskeleton reorganization, actin filament capping, cortical actin cytoskeleton organization, and small GTPase-mediated signal transduction pathways. In anterior capsules, proteins with significantly lower expression in ARC than in DC and PVC were involved in actin binding and actin cytoskeleton reorganization pathways.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>Actin cytoskeleton and actin-binding proteins are involved in lens fiber elongation and differentiation. 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Role of actin-binding proteins in cataract formation
Introduction
Actin has been implicated in lens opacification; however, the specific actin-related pathways involved in cataracts remain unelucidated. In this study, actin-related proteome changes and signaling pathways involved in the development of cataracts were evaluated.
Methods
The anterior capsule and phacoemulsification (phaco) cassette contents were collected during cataract surgery from 11 patients with diabetic cataract (DC), 12 patients with age-related cataract (ARC), and seven patients with post-vitrectomy cataract (PVC). Untargeted, global identification and quantification of proteins was performed through liquid chromatography-mass spectrometry with the data-independent acquisition (DIA).
Results
In phaco cassette samples, proteins with significantly lower expression in ARC than in DC and PVC were involved in various pathways, including actin binding, actin cytoskeleton reorganization, actin filament capping, cortical actin cytoskeleton organization, and small GTPase-mediated signal transduction pathways. In anterior capsules, proteins with significantly lower expression in ARC than in DC and PVC were involved in actin binding and actin cytoskeleton reorganization pathways.
Conclusion
Actin cytoskeleton and actin-binding proteins are involved in lens fiber elongation and differentiation. Rho GTPases contribute to actin cytoskeletal reorganization, and their inactivation is linked to abnormal lens fiber migration. These findings link actin binding to lens fiber integrity, lens opacification, and cataracts.
期刊介绍：
Cytoskeleton focuses on all aspects of cytoskeletal research in healthy and diseased states, spanning genetic and cell biological observations, biochemical, biophysical and structural studies, mathematical modeling and theory. This includes, but is certainly not limited to, classic polymer systems of eukaryotic cells and their structural sites of attachment on membranes and organelles, as well as the bacterial cytoskeleton, the nucleoskeleton, and uncoventional polymer systems with structural/organizational roles. Cytoskeleton is published in 12 issues annually, and special issues will be dedicated to especially-active or newly-emerging areas of cytoskeletal research.
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