以基因为中心的火山岛(意大利伊奥利亚群岛)土壤元基因组分析揭示了甲烷、氢和硫循环中的多种关键微生物。

IF 1.8 3区 生物学 Q4 MICROBIOLOGY
Federica Angius, Geert Cremers, Jeroen Frank, Caitlyn Witkowski, Arjan Pol, Theo A. van Alen, Mike S. M. Jetten, Huub J. M. Op den Camp, Tom Berben
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引用次数: 0

摘要

伊奥利亚群岛因其火山活动和热液排放而闻名于世,排放物主要是二氧化碳和硫化氢。氢气、甲烷和一氧化碳是这些排放物中的次要成分,它们共同滋养了大量的细菌和古细菌,而这些细菌和古细菌确实有助于清除这些臭名昭著的温室气体。在这里,我们分析了取自意大利武尔卡诺岛莱万特海湾样本的元基因组。采用以基因为中心的方法,热液喷口群落似乎以变形菌为主,硫单胞菌是含量最高的菌属。代谢重建突显了甲醛氧化和反向 TCA 循环在碳固定中的重要作用。[NiFe]-氢化酶似乎是氧化 H2 的首选策略,这表明除了 H2S 外,H2 也可能是该系统中必不可少的电子供体。此外,硫循环分析表明,硫酸盐还原基因的高丰度和多样性是产生 H2S 的基础。这项研究涵盖了莱万特海湾土壤微生物群落的多样性和代谢潜力,加深了我们对火山生态系统生物地球化学的了解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Gene-centered metagenome analysis of Vulcano Island soil (Aeolian archipelago, Italy) reveals diverse microbial key players in methane, hydrogen and sulfur cycles

Gene-centered metagenome analysis of Vulcano Island soil (Aeolian archipelago, Italy) reveals diverse microbial key players in methane, hydrogen and sulfur cycles

The Aeolian archipelago is known worldwide for its volcanic activity and hydrothermal emissions, of mainly carbon dioxide and hydrogen sulfide. Hydrogen, methane, and carbon monoxide are minor components of these emissions which together can feed large quantities of bacteria and archaea that do contribute to the removal of these notorious greenhouse gases. Here we analyzed the metagenome of samples taken from the Levante bay on Vulcano Island, Italy. Using a gene-centric approach, the hydrothermal vent community appeared to be dominated by Proteobacteria, and Sulfurimonas was the most abundant genus. Metabolic reconstructions highlight a prominent role of formaldehyde oxidation and the reverse TCA cycle in carbon fixation. [NiFe]-hydrogenases seemed to constitute the preferred strategy to oxidize H2, indicating that besides H2S, H2 could be an essential electron donor in this system. Moreover, the sulfur cycle analysis showed a high abundance and diversity of sulfate reduction genes underpinning the H2S production. This study covers the diversity and metabolic potential of the microbial soil community in Levante bay and adds to our understanding of the biogeochemistry of volcanic ecosystems.

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来源期刊
CiteScore
5.60
自引率
11.50%
发文量
104
审稿时长
3 months
期刊介绍: Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.
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