Hamed A Alshawkani, Mohamed Mansy, Mahmoud Al Ankily, Mohamed Shamel
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The odontogenic differentiation potential was analyzed using Alizarin red staining, RT-qPCR analysis of odontogenic genes DMP-1, DSPP, and alkaline phosphatase (ALP), and western blot analysis for detecting BMP-2 and RUNX-2 protein expression. An analysis of variance (ANOVA) followed by a <i>post hoc</i> <i>t</i>-test was employed to examine and compare the mean values of the results.</p><p><strong>Results: </strong>The study showed a notable rise in cell viability when NP and PBM were used together. Odontogenic gene expression and the protein expression of BMP-2 and RUNX-2 were notably increased in the combined group. 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引用次数: 0
摘要
目的：本研究旨在评估使用生物陶瓷密封剂 NeoPutty 和光生物调制（PBM）对牙髓干细胞（DPSCs）进行牙体生成的协同效应：从健康人的 10 颗前臼齿中采集牙髓干细胞。使用倒置相显微镜检测细胞形状，并使用流式细胞术检测干细胞特异性表面抗原，对牙髓干细胞进行特征描述。实验分为三组：NP组、PBM组以及NP和PBM联合组。3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑（MTT）实验用于评估DPSCs的活力。利用茜素红染色、牙源基因 DMP-1、DSPP 和碱性磷酸酶（ALP）的 RT-qPCR 分析以及检测 BMP-2 和 RUNX-2 蛋白表达的 Western 印迹分析，对牙源性分化潜能进行了分析。采用方差分析（ANOVA）和事后t检验来检验和比较结果的平均值：研究结果表明，当 NP 和 PBM 同时使用时，细胞活力明显提高。联合组的牙源性基因表达以及 BMP-2 和 RUNX-2 蛋白表达明显增加。NeoPutty 和 PBM 的联合作用在增强 DPSCs 的牙源性分化能力方面效果显著：结论：NeoPutty 和 PBM 的协同作用对 DPSCs 的细胞相容性和牙本质分化潜能产生了最积极的影响：临床意义：创造创新的再生疗法，高效持久地修复损伤的牙组织。本文引用方式Alshawkani HA, Mansy M, Al Ankily M, et al：体外研究。J Contemp Dent Pract 2024;25(4):313-319.
Regenerative Potential of Dental Pulp Stem Cells in Response to a Bioceramic Dental Sealer and Photobiomodulation: An In Vitro Study.
Aims: This study aims to assess the synergistic effect of utilizing a bioceramic sealer, NeoPutty, with photobiomodulation (PBM) on dental pulp stem cells (DPSCs) for odontogenesis.
Materials and methods: Dental pulp stem cells were collected from 10 premolars extracted from healthy individuals. Dental pulp stem cells were characterized using an inverted-phase microscope to detect cell shape and flow cytometry to detect stem cell-specific surface antigens. Three experimental groups were examined: the NP group, the PBM group, and the combined NP and PBM group. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) experiment was conducted to assess the viability of DPSCs. The odontogenic differentiation potential was analyzed using Alizarin red staining, RT-qPCR analysis of odontogenic genes DMP-1, DSPP, and alkaline phosphatase (ALP), and western blot analysis for detecting BMP-2 and RUNX-2 protein expression. An analysis of variance (ANOVA) followed by a post hoct-test was employed to examine and compare the mean values of the results.
Results: The study showed a notable rise in cell viability when NP and PBM were used together. Odontogenic gene expression and the protein expression of BMP-2 and RUNX-2 were notably increased in the combined group. The combined effect of NeoPutty and PBM was significant in enhancing the odontogenic differentiation capability of DPSCs.
Conclusion: The synergistic effect of NeoPutty and PBM produced the most positive effect on the cytocompatibility and odontogenic differentiation potential of DPSCs.
Clinical significance: Creating innovative regenerative treatments to efficiently and durably repair injured dental tissues. How to cite this article: Alshawkani HA, Mansy M, Al Ankily M, et al. Regenerative Potential of Dental Pulp Stem Cells in Response to a Bioceramic Dental Sealer and Photobiomodulation: An In Vitro Study. J Contemp Dent Pract 2024;25(4):313-319.
期刊介绍：
The Journal of Contemporary Dental Practice (JCDP), is a peer-reviewed, open access MEDLINE indexed journal. The journal’s full text is available online at http://www.thejcdp.com. The journal allows free access (open access) to its contents. Articles with clinical relevance will be given preference for publication. The Journal publishes original research papers, review articles, rare and novel case reports, and clinical techniques. Manuscripts are invited from all specialties of dentistry i.e., conservative dentistry and endodontics, dentofacial orthopedics and orthodontics, oral medicine and radiology, oral pathology, oral surgery, orodental diseases, pediatric dentistry, implantology, periodontics, clinical aspects of public health dentistry, and prosthodontics.
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