Zanthoxylum armatum DC 中 BZR 基因家族的全基因组鉴定和表达分析,以及 ZaBZR1 在抗旱中的功能分析。

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2024-07-02 DOI:10.1007/s00425-024-04469-0
Zhengyu Jin, Tao Zhou, Jiajia Chen, Chaoting Lang, Qingqing Zhang, Jin Qin, Haibo Lan, Jianrong Li, Xiaofang Zeng
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引用次数: 0

摘要

主要结论本研究发现了6个ZaBZRs,所有ZaBZRs均受脱落酸(ABA)和干旱的上调。ZaBZR1 的过表达增强了转基因烟草的耐旱性。芸苔素甾醇(BRs)是植物体内一类重要的甾醇激素,在植物的生长发育过程中起着至关重要的作用。BZR(抗芸苔素类固醇)是芸苔素类固醇信号转导途径中的一个重要转录因子。然而,目前尚未在 Zanthoxylum armatum DC 中发现 BZR 基因家族成员。本研究通过生物信息学方法鉴定了 ZaBZR 家族的六个成员。所有六个 ZaBZRs 都有多个磷酸化位点。系统发育和共线性分析表明,ZaBZRs 与位于 B 亚基因组上的 ZbBZRs 关系最为密切。表达分析表明,ZaBZRs 在Z. armatum 中具有组织特异性表达模式,其启动子区域包含与激素反应和胁迫诱导相关的顺式作用元件。此外,所有六个 ZaBZRs 在脱落酸(ABA)和聚乙二醇(PEG)处理后都出现上调,表明它们参与了干旱响应。随后,我们对 ZaBZR1 进行了广泛的研究。ZaBZR1 在根中的表达量最高,其次是茎和顶芽。亚细胞定位分析表明,ZaBZR1 存在于细胞质和细胞核中。与野生型植物相比,在转基因烟草中过表达 ZaBZR1 能提高种子萌发率和干旱条件下的根系生长,降低水分损失率。此外,ZaBZR1 增加了脯氨酸含量(PRO),降低了丙二醛含量(MDA),表明对干旱引起的氧化应激的耐受性有所提高。转基因植物还减少了活性氧的积累。重要的是,ZaBZR1 上调了干旱相关基因的表达,如 NbP5CS1、NbDREB2A 和 NbWRKY44。这些发现凸显了 ZaBZR1 作为增强转基因 N. benthamiana 抗旱性候选基因的潜力,并为 Z. armatum 中 ZaBZRs 的功能提供了见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Genome-wide identification and expression analysis of the BZR gene family in Zanthoxylum armatum DC and functional analysis of ZaBZR1 in drought tolerance.

Genome-wide identification and expression analysis of the BZR gene family in Zanthoxylum armatum DC and functional analysis of ZaBZR1 in drought tolerance.

Main conclusion: In this study, six ZaBZRs were identified in Zanthoxylum armatum DC, and all the ZaBZRs were upregulated by abscisic acid (ABA) and drought. Overexpression of ZaBZR1 enhanced the drought tolerance of transgenic Nicotiana benthamian. Brassinosteroids (BRs) are a pivotal class of sterol hormones in plants that play a crucial role in plant growth and development. BZR (brassinazole resistant) is a crucial transcription factor in the signal transduction pathway of BRs. However, the BZR gene family members have not yet been identified in Zanthoxylum armatum DC. In this study, six members of the ZaBZR family were identified by bioinformatic methods. All six ZaBZRs exhibited multiple phosphorylation sites. Phylogenetic and collinearity analyses revealed a closest relationship between ZaBZRs and ZbBZRs located on the B subgenomes. Expression analysis revealed tissue-specific expression patterns of ZaBZRs in Z. armatum, and their promoter regions contained cis-acting elements associated with hormone response and stress induction. Additionally, all six ZaBZRs showed upregulation upon treatment after abscisic acid (ABA) and polyethylene glycol (PEG), indicating their participation in drought response. Subsequently, we conducted an extensive investigation of ZaBZR1. ZaBZR1 showed the highest expression in the root, followed by the stem and terminal bud. Subcellular localization analysis revealed that ZaBZR1 is present in the cytoplasm and nucleus. Overexpression of ZaBZR1 in transgenic Nicotiana benthamiana improved seed germination rate and root growth under drought conditions, reducing water loss rates compared to wild-type plants. Furthermore, ZaBZR1 increased proline content (PRO) and decreased malondialdehyde content (MDA), indicating improved tolerance to drought-induced oxidative stress. The transgenic plants also showed a reduced accumulation of reactive oxygen species. Importantly, ZaBZR1 up-regulated the expression of drought-related genes such as NbP5CS1, NbDREB2A, and NbWRKY44. These findings highlight the potential of ZaBZR1 as a candidate gene for enhancing drought resistance in transgenic N. benthamiana and provide insight into the function of ZaBZRs in Z. armatum.

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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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