一种新的生育力恢复基因 Rf20 的结构变异是恢复水稻野生败育型细胞质雄性不育的基础。

IF 17.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-07-02 DOI:10.1016/j.molp.2024.07.001
Shufeng Song, Yixing Li, Mudan Qiu, Na Xu, Bin Li, Longhui Zhang, Lei Li, Weijun Chen, Jinglei Li, Tiankang Wang, Yingxin Qiu, Mengmeng Gong, Dong Yu, Hao Dong, Siqi Xia, Yi Pan, Dingyang Yuan, Li Li
{"title":"一种新的生育力恢复基因 Rf20 的结构变异是恢复水稻野生败育型细胞质雄性不育的基础。","authors":"Shufeng Song, Yixing Li, Mudan Qiu, Na Xu, Bin Li, Longhui Zhang, Lei Li, Weijun Chen, Jinglei Li, Tiankang Wang, Yingxin Qiu, Mengmeng Gong, Dong Yu, Hao Dong, Siqi Xia, Yi Pan, Dingyang Yuan, Li Li","doi":"10.1016/j.molp.2024.07.001","DOIUrl":null,"url":null,"abstract":"<p><p>The discovery of a wild abortive-type (WA) cytoplasmic male sterile (CMS) line and breeding its restorer line have led to the commercialization of three-line hybrid rice, contributing considerably to global food security. However, the molecular mechanisms underlying fertility abortion and the restoration of CMS-WA lines remain largely elusive. In this study, we cloned a restorer gene, Rf20, following a genome-wide association study analysis of the core parent lines of three-line hybrid rice. We found that Rf20 was present in all core parental lines, but different haplotypes and structural variants of its gene resulted in differences in Rf20 expression levels between sterile and restored lines. Rf20 could restore pollen fertility in the CMS-WA line and was found to be responsible for fertility restoration in some CMS lines under high temperatures. In addition, we found that Rf20 encodes a pentatricopeptide repeat protein that competes with WA352 for binding with COX11. This interaction enhances COX11's function as a scavenger of reactive oxygen species, which in turn restores pollen fertility. Collectively, our study suggests a new action mode for pentatricopeptide repeat proteins in the fertility restoration of CMS lines, providing an essential theoretical basis for breeding robust restorer lines and for overcoming high temperature-induced fertility recovery of some CMS lines.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1272-1288"},"PeriodicalIF":17.1000,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Structural variations of a new fertility restorer gene, Rf20, underlie the restoration of wild abortive-type cytoplasmic male sterility in rice.\",\"authors\":\"Shufeng Song, Yixing Li, Mudan Qiu, Na Xu, Bin Li, Longhui Zhang, Lei Li, Weijun Chen, Jinglei Li, Tiankang Wang, Yingxin Qiu, Mengmeng Gong, Dong Yu, Hao Dong, Siqi Xia, Yi Pan, Dingyang Yuan, Li Li\",\"doi\":\"10.1016/j.molp.2024.07.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The discovery of a wild abortive-type (WA) cytoplasmic male sterile (CMS) line and breeding its restorer line have led to the commercialization of three-line hybrid rice, contributing considerably to global food security. However, the molecular mechanisms underlying fertility abortion and the restoration of CMS-WA lines remain largely elusive. In this study, we cloned a restorer gene, Rf20, following a genome-wide association study analysis of the core parent lines of three-line hybrid rice. We found that Rf20 was present in all core parental lines, but different haplotypes and structural variants of its gene resulted in differences in Rf20 expression levels between sterile and restored lines. Rf20 could restore pollen fertility in the CMS-WA line and was found to be responsible for fertility restoration in some CMS lines under high temperatures. In addition, we found that Rf20 encodes a pentatricopeptide repeat protein that competes with WA352 for binding with COX11. This interaction enhances COX11's function as a scavenger of reactive oxygen species, which in turn restores pollen fertility. Collectively, our study suggests a new action mode for pentatricopeptide repeat proteins in the fertility restoration of CMS lines, providing an essential theoretical basis for breeding robust restorer lines and for overcoming high temperature-induced fertility recovery of some CMS lines.</p>\",\"PeriodicalId\":19012,\"journal\":{\"name\":\"Molecular Plant\",\"volume\":\" \",\"pages\":\"1272-1288\"},\"PeriodicalIF\":17.1000,\"publicationDate\":\"2024-08-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Plant\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1016/j.molp.2024.07.001\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/7/2 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Plant","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.molp.2024.07.001","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/7/2 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

野生败育型细胞质雄性不育系的发现及其恢复系的培育促成了三系杂交水稻的商业化,为全球粮食安全做出了巨大贡献。然而,野生败育型细胞质雄性不育系的育性败育和恢复系的分子机理在很大程度上仍然是未知的。在本研究中,我们对三系杂交水稻的核心亲本品系进行了全基因组关联研究分析,克隆了一个恢复基因 Rf20。我们发现 Rf20 存在于所有核心亲本品系中,但其基因的单倍型和结构变异导致不育系和恢复系的 Rf20 表达水平不同。Rf20能恢复野生败育型细胞质雄性不育系的育性,并在高温条件下对一些细胞质雄性不育系的育性恢复起作用。此外,我们还发现 Rf20 编码的五三肽重复蛋白与 WA352 竞争性地与 COX11 结合。这种相互作用增强了 COX11 作为活性氧清除剂的功能,进而恢复了花粉的生育能力。本研究提出了一个参与细胞质雄性不育系育性恢复的五叉肽重复蛋白新模型,为培育强恢复系和克服某些三系不育系的高温育性恢复提供了重要的理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Structural variations of a new fertility restorer gene, Rf20, underlie the restoration of wild abortive-type cytoplasmic male sterility in rice.

The discovery of a wild abortive-type (WA) cytoplasmic male sterile (CMS) line and breeding its restorer line have led to the commercialization of three-line hybrid rice, contributing considerably to global food security. However, the molecular mechanisms underlying fertility abortion and the restoration of CMS-WA lines remain largely elusive. In this study, we cloned a restorer gene, Rf20, following a genome-wide association study analysis of the core parent lines of three-line hybrid rice. We found that Rf20 was present in all core parental lines, but different haplotypes and structural variants of its gene resulted in differences in Rf20 expression levels between sterile and restored lines. Rf20 could restore pollen fertility in the CMS-WA line and was found to be responsible for fertility restoration in some CMS lines under high temperatures. In addition, we found that Rf20 encodes a pentatricopeptide repeat protein that competes with WA352 for binding with COX11. This interaction enhances COX11's function as a scavenger of reactive oxygen species, which in turn restores pollen fertility. Collectively, our study suggests a new action mode for pentatricopeptide repeat proteins in the fertility restoration of CMS lines, providing an essential theoretical basis for breeding robust restorer lines and for overcoming high temperature-induced fertility recovery of some CMS lines.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Molecular Plant
Molecular Plant 植物科学-生化与分子生物学
CiteScore
37.60
自引率
2.20%
发文量
1784
审稿时长
1 months
期刊介绍: Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信