同时具有高灵敏度和高速度的声流体增强生物传感技术。

IF 7.3 1区 工程技术 Q1 INSTRUMENTS & INSTRUMENTATION
Microsystems & Nanoengineering Pub Date : 2024-06-29 eCollection Date: 2024-01-01 DOI:10.1038/s41378-024-00731-3
Yuang Li, Yang Zhao, Yang Yang, Wenchang Zhang, Yun Zhang, Sheng Sun, Lingqian Zhang, Mingxiao Li, Hang Gao, Chengjun Huang
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引用次数: 0

摘要

同时实现高灵敏度和检测速度的传统固态生物传感器通常受到限制,因为目标分子必须在被动扩散到传感器表面后才能被检测到。微流控技术可使分子持续通过生物传感区域,从而缩短扩散时间。然而,生物分子的结合效率仍然受到微米级通道内固有层流的限制。本研究将聚焦行进表面声波导入声学微流控芯片,可将目标分子持续富集到收缩区,即时检测免疫反应,从而显著提高检测灵敏度和速度。为了证明生物传感的增强效果,我们首先开发了一种集成了聚焦齿间换能器的声学微流控芯片,这种换能器能够捕获 91% 以上通过的微珠。随后,聚苯乙烯微珠预先捕获了不同浓度的人类 IgG 分子,并装载到芯片上进行检测。具有代表性的结果是,在浓度为 100、10、1 和 0.1 纳克/毫升(约 0.7 pM)时,分别需要约 0.63、2.62、11.78 和 19.75 秒才能积聚大量预先捕获人类 IgG 分子的微珠;在小时水平上,这一过程比其他方法更快,在纳摩尔水平上,比其他方法更灵敏。我们的结果表明,所提出的方法能显著提高灵敏度和速度,揭示了选择性富集策略对稀有分子快速生物传感的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Acoustofluidics-enhanced biosensing with simultaneously high sensitivity and speed.

Acoustofluidics-enhanced biosensing with simultaneously high sensitivity and speed.

Simultaneously achieving high sensitivity and detection speed with traditional solid-state biosensors is usually limited since the target molecules must passively diffuse to the sensor surface before they can be detected. Microfluidic techniques have been applied to shorten the diffusion time by continuously moving molecules through the biosensing regions. However, the binding efficiencies of the biomolecules are still limited by the inherent laminar flow inside microscale channels. In this study, focused traveling surface acoustic waves were directed into an acoustic microfluidic chip, which could continuously enrich the target molecules into a constriction zone for immediate detection of the immune reactions, thus significantly improving the detection sensitivity and speed. To demonstrate the enhancement of biosensing, we first developed an acoustic microfluidic chip integrated with a focused interdigital transducer; this transducer had the ability to capture more than 91% of passed microbeads. Subsequently, polystyrene microbeads were pre-captured with human IgG molecules at different concentrations and loaded for detection on the chip. As representative results, ~0.63, 2.62, 11.78, and 19.75 seconds were needed to accumulate significant numbers of microbeads pre-captured with human IgG molecules at concentrations of 100, 10, 1, and 0.1 ng/mL (~0.7 pM), respectively; this process was faster than the other methods at the hour level and more sensitive than the other methods at the nanomolar level. Our results indicated that the proposed method could significantly improve both the sensitivity and speed, revealing the importance of selective enrichment strategies for rapid biosensing of rare molecules.

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来源期刊
Microsystems & Nanoengineering
Microsystems & Nanoengineering Materials Science-Materials Science (miscellaneous)
CiteScore
12.00
自引率
3.80%
发文量
123
审稿时长
20 weeks
期刊介绍: Microsystems & Nanoengineering is a comprehensive online journal that focuses on the field of Micro and Nano Electro Mechanical Systems (MEMS and NEMS). It provides a platform for researchers to share their original research findings and review articles in this area. The journal covers a wide range of topics, from fundamental research to practical applications. Published by Springer Nature, in collaboration with the Aerospace Information Research Institute, Chinese Academy of Sciences, and with the support of the State Key Laboratory of Transducer Technology, it is an esteemed publication in the field. As an open access journal, it offers free access to its content, allowing readers from around the world to benefit from the latest developments in MEMS and NEMS.
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