急性紫外线 B 照射会增加雌性小鼠肝脏中的胆固醇并降低 Cyp7a1 的表达。

IF 1.8 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Lipids Pub Date : 2024-07-03 DOI:10.1002/lipd.12407

Takumi Yamane, Takahiro Okumoto, Tomoko Tamura, Yuichi Oishi

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引用次数: 0

摘要

最近的研究表明，紫外线 B（UVB）照射会影响皮肤和肝脏功能。在这项研究中，我们调查了紫外线 B 照射对肝脏胆固醇代谢的影响。将无毛小鼠置于紫外线（1.6 J/cm2）照射下。照射24小时后收集背侧皮肤和肝脏样本。从皮肤和肝脏组织中提取总 RNA，用于 DNA 微阵列分析和实时聚合酶链反应（PCR）。与 UVB（-）组相比，UVB（+）组肝脏中 Cyp7a1 的 mRNA 表达量减少了 4.4 倍。与胆固醇代谢相关的其他基因的表达量未见差异。此外，UVB（+）组肝脏总胆固醇水平明显高于 UVB（-）组。这些研究结果表明，急性 UVB 照射会增加肝脏中的总胆固醇水平并降低 Cyp7a1 的表达。

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Acute ultraviolet B irradiation increases cholesterol and decreases Cyp7a1 expression in the liver of female mice

Recent studies have demonstrated that ultraviolet B (UVB) irradiation impacts both skin and hepatic functions. In this study, we investigated the effects of UVB irradiation on cholesterol metabolism in the liver. Hairless mice were exposed to UVB (1.6 J/cm²) irradiation. Dorsal skin and liver samples were collected 24 h after exposure. Total RNA was extracted from the skin and liver tissues, and used for DNA microarray analysis and real-time polymerase chain reaction (PCR). Hepatic mRNA expression of Cyp7a1 revealed a 4.4-fold decrease in the UVB (+) group compared to that in the UVB (−) group. No differences were observed in the expression of the other genes related to cholesterol metabolism. Additionally, the level of hepatic total cholesterol in the UVB (+) group was significantly higher than in the UVB (−) group. These findings suggest that acute UVB irradiation increases total cholesterol levels and decreases Cyp7a1 expression in the liver.

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来源期刊

Lipids 生物-生化与分子生物学

CiteScore

4.20

自引率

5.30%

发文量

审稿时长

4-8 weeks

期刊介绍： Lipids is a journal of the American Oil Chemists'' Society (AOCS) that focuses on publishing high-quality peer-reviewed papers and invited reviews in the general area of lipid research, including chemistry, biochemistry, clinical nutrition, and metabolism. In addition, Lipids publishes papers establishing novel methods for addressing research questions in the field of lipid research.