{"title":"Juglans mandshurica Maxim 未成熟果实果皮中富含半乳糖基和葡萄糖基的复合果胶的结构阐释","authors":"Xi-Zhe Sun, Qing-Yu Zhang, Si-Liang Jiang, Rong-Jian Zhu, Jun-Hong Chai, Jun Liang, Hai-Xue Kuang, Yong-Gang Xia","doi":"10.1007/s10719-024-10156-9","DOIUrl":null,"url":null,"abstract":"<p><p>A glucosyl-rich pectin, JMMP-3 (M<sub>w</sub>, 2.572 × 10<sup>4</sup> g/mol, O-methyl % = 3.62%), was isolated and purified from the pericarp of the immature fruit of Juglans mandshurica Maxim. (QingLongYi). The structure of JMMP-3 was studied systematically by infrared spectroscopy, monosaccharide compositions, methylation analysis, partial acid hydrolysis, and 1/2D-NMR. The backbone of JMMP-3 possessed a smooth region (→ <sup>4</sup>GalA<sup>1</sup> →) and a hairy region (→ <sup>4</sup>GalA<sup>1</sup> → <sup>2</sup>Rha<sup>1</sup> →) with a molar ratio of 2: 5. The substitution of four characteristic side chains (R<sub>1</sub>-R<sub>4</sub>) occurs at C-4 of → 2,4)-α-Rhap-(1→, where R<sub>1</sub> is composed of → 5)-α-Araf-(1→, R<sub>2</sub> is composed of → 4)-β-Galp-(1 → and β-Galp-(1→, R<sub>3</sub> is composed of α-Glcp-(1→, →4)-α-Glcp-(1 → and → 4,6)-α-Glcp-(1→, and R<sub>4</sub> is composed of → 5)-α-Araf-(1→, β-Galp-(1→, → 4)-β-Galp-(1→, → 3,4)-β-Galp-(1→, → 4,6)-β-Galp-(1 → and → 2,4)-β-Galp-(1 → . In addition, the antitumor activity of JMMP-3 on HepG2 cells was preliminarily investigated.</p>","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":" ","pages":"201-216"},"PeriodicalIF":2.7000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Structural elucidation a complex galactosyl and glucosyl-rich pectin from the pericarp of immature fruits of Juglans mandshurica Maxim.\",\"authors\":\"Xi-Zhe Sun, Qing-Yu Zhang, Si-Liang Jiang, Rong-Jian Zhu, Jun-Hong Chai, Jun Liang, Hai-Xue Kuang, Yong-Gang Xia\",\"doi\":\"10.1007/s10719-024-10156-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A glucosyl-rich pectin, JMMP-3 (M<sub>w</sub>, 2.572 × 10<sup>4</sup> g/mol, O-methyl % = 3.62%), was isolated and purified from the pericarp of the immature fruit of Juglans mandshurica Maxim. (QingLongYi). The structure of JMMP-3 was studied systematically by infrared spectroscopy, monosaccharide compositions, methylation analysis, partial acid hydrolysis, and 1/2D-NMR. The backbone of JMMP-3 possessed a smooth region (→ <sup>4</sup>GalA<sup>1</sup> →) and a hairy region (→ <sup>4</sup>GalA<sup>1</sup> → <sup>2</sup>Rha<sup>1</sup> →) with a molar ratio of 2: 5. The substitution of four characteristic side chains (R<sub>1</sub>-R<sub>4</sub>) occurs at C-4 of → 2,4)-α-Rhap-(1→, where R<sub>1</sub> is composed of → 5)-α-Araf-(1→, R<sub>2</sub> is composed of → 4)-β-Galp-(1 → and β-Galp-(1→, R<sub>3</sub> is composed of α-Glcp-(1→, →4)-α-Glcp-(1 → and → 4,6)-α-Glcp-(1→, and R<sub>4</sub> is composed of → 5)-α-Araf-(1→, β-Galp-(1→, → 4)-β-Galp-(1→, → 3,4)-β-Galp-(1→, → 4,6)-β-Galp-(1 → and → 2,4)-β-Galp-(1 → . In addition, the antitumor activity of JMMP-3 on HepG2 cells was preliminarily investigated.</p>\",\"PeriodicalId\":12762,\"journal\":{\"name\":\"Glycoconjugate Journal\",\"volume\":\" \",\"pages\":\"201-216\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Glycoconjugate Journal\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s10719-024-10156-9\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/7/2 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Glycoconjugate Journal","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10719-024-10156-9","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/7/2 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Structural elucidation a complex galactosyl and glucosyl-rich pectin from the pericarp of immature fruits of Juglans mandshurica Maxim.
A glucosyl-rich pectin, JMMP-3 (Mw, 2.572 × 104 g/mol, O-methyl % = 3.62%), was isolated and purified from the pericarp of the immature fruit of Juglans mandshurica Maxim. (QingLongYi). The structure of JMMP-3 was studied systematically by infrared spectroscopy, monosaccharide compositions, methylation analysis, partial acid hydrolysis, and 1/2D-NMR. The backbone of JMMP-3 possessed a smooth region (→ 4GalA1 →) and a hairy region (→ 4GalA1 → 2Rha1 →) with a molar ratio of 2: 5. The substitution of four characteristic side chains (R1-R4) occurs at C-4 of → 2,4)-α-Rhap-(1→, where R1 is composed of → 5)-α-Araf-(1→, R2 is composed of → 4)-β-Galp-(1 → and β-Galp-(1→, R3 is composed of α-Glcp-(1→, →4)-α-Glcp-(1 → and → 4,6)-α-Glcp-(1→, and R4 is composed of → 5)-α-Araf-(1→, β-Galp-(1→, → 4)-β-Galp-(1→, → 3,4)-β-Galp-(1→, → 4,6)-β-Galp-(1 → and → 2,4)-β-Galp-(1 → . In addition, the antitumor activity of JMMP-3 on HepG2 cells was preliminarily investigated.
期刊介绍：
Glycoconjugate Journal publishes articles and reviews on all areas concerned with:
function, composition, structure, biosynthesis, degradation, interactions, recognition and chemo-enzymatic synthesis of glycoconjugates (glycoproteins, glycolipids, oligosaccharides, polysaccharides and proteoglycans), biochemistry, molecular biology, biotechnology, immunology and cell biology of glycoconjugates, aspects related to disease processes (immunological, inflammatory, arthritic infections, metabolic disorders, malignancy, neurological disorders), structural and functional glycomics, glycoimmunology, glycovaccines, organic synthesis of glycoconjugates and the development of methodologies if biologically relevant, glycosylation changes in disease if focused on either the discovery of a novel disease marker or the improved understanding of some basic pathological mechanism, articles on the effects of toxicological agents (alcohol, tobacco, narcotics, environmental agents) on glycosylation, and the use of glycotherapeutics.
Glycoconjugate Journal is the official journal of the International Glycoconjugate Organization, which is responsible for organizing the biennial International Symposia on Glycoconjugates.
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