脊髓灰质炎病毒壳蛋白 VP3 可以穿透血管内皮细胞。

IF 3.5 4区 生物学 Q1 Biochemistry, Genetics and Molecular Biology
Taketoshi Mizutani, Aya Ishizaka
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引用次数: 0

摘要

脊髓灰质炎病毒(PV)通过血液进入中枢神经系统(CNS),这表明存在一种穿越血脑屏障的机制。在这里,我们报告了脊髓灰质炎病毒的囊膜蛋白(VP1 和 VP3)可以穿透细胞,其中 VP3 的侵袭性更强。VP3 的两个独立部分负责这一功能。两种肽都能穿透人脐带血管内皮细胞,VP3的一种肽还能穿透外周血单核细胞。在使用大鼠来源的星形胶质细胞、周细胞和内皮细胞进行的体外血脑屏障模型中,观察到这两种肽都能在给药后 6 小时从血液一侧穿透到大脑一侧。这些研究结果为我们提供了深入了解葡萄球菌侵入中枢神经系统的分子机制的信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Poliovirus capsid protein VP3 can penetrate vascular endothelial cells

Poliovirus capsid protein VP3 can penetrate vascular endothelial cells

The poliovirus (PV) enters the central nervous system (CNS) via the bloodstream, suggesting the existence of a mechanism to cross the blood–brain barrier. Here, we report that PV capsid proteins (VP1 and VP3) can penetrate cells, with VP3 being more invasive. Two independent parts of VP3 are responsible for this function. Both peptides can penetrate human umbilical cord vascular endothelial cells, and one peptide of VP3 could also penetrate peripheral blood mononuclear cells. In an in vitro blood–brain barrier model using rat-derived astrocytes, pericytes, and endothelial cells, both peptides were observed to traverse from the blood side to the brain side at 6 h after administration. These results provide insights into the molecular mechanisms underlying PV invasion into the CNS.

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来源期刊
FEBS Letters
FEBS Letters 生物-生化与分子生物学
CiteScore
7.00
自引率
2.90%
发文量
303
审稿时长
1.0 months
期刊介绍: FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.
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