{"title":"广谱沙门氏菌噬菌体的分离、全基因组测序和应用。","authors":"Xiaofeng Zheng, Xin Wang, Yu Zhou, Meihan Liu, Pei Li, Linyun Gao, Hui Wang, Xuelian Ma, Liqun Wang, Xiang Huo, Wei Zhang","doi":"10.1007/s00203-024-04061-w","DOIUrl":null,"url":null,"abstract":"<div><p><i>Salmonella</i> is considered as one of the most common zoonotic /foodborne pathogens in the world. The application of bacteriophages as novel antibacterial agents in food substrates has become an emerging strategy. Bacteriophages have the potential to control <i>Salmonella</i> contamination.</p><p>We have isolated and characterized a broad-spectrum <i>Salmonella</i> phage, SP154, which can lyse 9 serotypes, including <i>S</i>. Enteritidis, <i>S</i>. Typhimurium, <i>S</i>. Pullorum, <i>S</i>. Arizonae, <i>S</i>. Dublin, <i>S</i>. Cholerasuis, <i>S</i>. Chester, <i>S</i>. 1, 4, [5], 12: i: –, and <i>S</i>. Derby, accounting for 81.9% of 144 isolates. SP154 showed a short latent period (40 min) and a high burst size (with the first rapid burst size at 107 PFUs/cell and the second rapid burst size at approximately 40 PFUs/cell). Furthermore, SP154 activity has higher survival rates across various environmental conditions, including pH 4.0–12.0 and temperatures ranging from 4 to 50 °C for 60 min, making it suitable for diverse food processing and storage applications. Significant reductions in live <i>Salmonella</i> were observed in different foods matrices such as milk and chicken meat, with a decrease of up to 1.9 log<sub>10</sub> CFU/mL in milk contamination and a 1 log<sub>10</sub> CFU/mL reduction in chicken meat. Whole genome sequencing analysis revealed that SP154 belongs to the genus <i>Ithacavirus</i>, subfamily <i>Humphriesvirinae</i>, within the family <i>Schitoviridae</i>. Phylogenetic analysis based on the terminase large subunit supported this classification, although an alternate tree using the tail spike protein gene suggested affiliation with the genus <i>Kuttervirus</i>, underscoring the limitations of relying on a single gene for phylogenetic inference. Importantly, no virulence or antibiotic resistance genes were detected in SP154. Our research highlights the potential of using SP154 for biocontrol of <i>Salmonella</i> in the food industry.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 7","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isolation, whole genome sequencing and application of a broad-spectrum Salmonella phage\",\"authors\":\"Xiaofeng Zheng, Xin Wang, Yu Zhou, Meihan Liu, Pei Li, Linyun Gao, Hui Wang, Xuelian Ma, Liqun Wang, Xiang Huo, Wei Zhang\",\"doi\":\"10.1007/s00203-024-04061-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><i>Salmonella</i> is considered as one of the most common zoonotic /foodborne pathogens in the world. The application of bacteriophages as novel antibacterial agents in food substrates has become an emerging strategy. Bacteriophages have the potential to control <i>Salmonella</i> contamination.</p><p>We have isolated and characterized a broad-spectrum <i>Salmonella</i> phage, SP154, which can lyse 9 serotypes, including <i>S</i>. Enteritidis, <i>S</i>. Typhimurium, <i>S</i>. Pullorum, <i>S</i>. Arizonae, <i>S</i>. Dublin, <i>S</i>. Cholerasuis, <i>S</i>. Chester, <i>S</i>. 1, 4, [5], 12: i: –, and <i>S</i>. Derby, accounting for 81.9% of 144 isolates. SP154 showed a short latent period (40 min) and a high burst size (with the first rapid burst size at 107 PFUs/cell and the second rapid burst size at approximately 40 PFUs/cell). Furthermore, SP154 activity has higher survival rates across various environmental conditions, including pH 4.0–12.0 and temperatures ranging from 4 to 50 °C for 60 min, making it suitable for diverse food processing and storage applications. Significant reductions in live <i>Salmonella</i> were observed in different foods matrices such as milk and chicken meat, with a decrease of up to 1.9 log<sub>10</sub> CFU/mL in milk contamination and a 1 log<sub>10</sub> CFU/mL reduction in chicken meat. Whole genome sequencing analysis revealed that SP154 belongs to the genus <i>Ithacavirus</i>, subfamily <i>Humphriesvirinae</i>, within the family <i>Schitoviridae</i>. Phylogenetic analysis based on the terminase large subunit supported this classification, although an alternate tree using the tail spike protein gene suggested affiliation with the genus <i>Kuttervirus</i>, underscoring the limitations of relying on a single gene for phylogenetic inference. Importantly, no virulence or antibiotic resistance genes were detected in SP154. Our research highlights the potential of using SP154 for biocontrol of <i>Salmonella</i> in the food industry.</p></div>\",\"PeriodicalId\":8279,\"journal\":{\"name\":\"Archives of Microbiology\",\"volume\":\"206 7\",\"pages\":\"\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-07-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archives of Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00203-024-04061-w\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s00203-024-04061-w","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Isolation, whole genome sequencing and application of a broad-spectrum Salmonella phage
Salmonella is considered as one of the most common zoonotic /foodborne pathogens in the world. The application of bacteriophages as novel antibacterial agents in food substrates has become an emerging strategy. Bacteriophages have the potential to control Salmonella contamination.
We have isolated and characterized a broad-spectrum Salmonella phage, SP154, which can lyse 9 serotypes, including S. Enteritidis, S. Typhimurium, S. Pullorum, S. Arizonae, S. Dublin, S. Cholerasuis, S. Chester, S. 1, 4, [5], 12: i: –, and S. Derby, accounting for 81.9% of 144 isolates. SP154 showed a short latent period (40 min) and a high burst size (with the first rapid burst size at 107 PFUs/cell and the second rapid burst size at approximately 40 PFUs/cell). Furthermore, SP154 activity has higher survival rates across various environmental conditions, including pH 4.0–12.0 and temperatures ranging from 4 to 50 °C for 60 min, making it suitable for diverse food processing and storage applications. Significant reductions in live Salmonella were observed in different foods matrices such as milk and chicken meat, with a decrease of up to 1.9 log10 CFU/mL in milk contamination and a 1 log10 CFU/mL reduction in chicken meat. Whole genome sequencing analysis revealed that SP154 belongs to the genus Ithacavirus, subfamily Humphriesvirinae, within the family Schitoviridae. Phylogenetic analysis based on the terminase large subunit supported this classification, although an alternate tree using the tail spike protein gene suggested affiliation with the genus Kuttervirus, underscoring the limitations of relying on a single gene for phylogenetic inference. Importantly, no virulence or antibiotic resistance genes were detected in SP154. Our research highlights the potential of using SP154 for biocontrol of Salmonella in the food industry.
期刊介绍:
Research papers must make a significant and original contribution to
microbiology and be of interest to a broad readership. The results of any
experimental approach that meets these objectives are welcome, particularly
biochemical, molecular genetic, physiological, and/or physical investigations into
microbial cells and their interactions with their environments, including their eukaryotic hosts.
Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published.
Theoretical papers and those that report on the analysis or ''mining'' of data are
acceptable in principle if new information, interpretations, or hypotheses
emerge.