基因组分析将美洲水貂的皇家粉色被毛表型与 HPS3 基因中的 1 型逆转录病毒元件插入联系起来。

IF 1.8 3区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal genetics Pub Date : 2024-07-02 DOI:10.1111/age.13461
Andrey D. Manakhov, Nelli A. Aarakelyan, Adela V. Lapteacru, Tatiana V. Andreeva, Oleg V. Trapezov, Evgeny I. Rogaev
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引用次数: 0

摘要

迄今为止,在美洲水貂(Neogale vison)身上观察到的 30 多种毛色中,只有 10 种与特定基因有关。皇家粉彩毛色属于棕色毛色大家族的一部分,这些毛色彼此非常相似,这就增加了育种和选种过程的难度。在这里,我们对来自两个不同水貂种群的五只具有皇家粉彩(b/b)表型的美洲水貂进行了全基因组测序。我们在编码 HPS3 蛋白的基因的第一个内含子中发现了内源性逆转录病毒 1 型元件(ERV1)的插入。通过Cas9靶向纳米孔测序,我们重建了11.7 Kb ERV1插入物的全长序列,并观察到了扩散到HPS3基因启动子区域的高甲基化。这些发现突显了 HPS3 在黑色素体和黑色素形成过程中的作用,以及调节毛色强度和光谱的遗传过程。此外,在水貂育种项目中,这些数据还有助于跟踪具有重要经济价值的毛皮质量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genomic analysis links the American mink Royal pastel coat phenotype to retroviral element type 1 insertion in the HPS3 gene

To date, only 10 of the more than 30 fur colours that had been observed in American mink (Neogale vison) have been linked to specific genes. The Royal pastel fur colour is part of a large family of brownish colours that are quite similar to one another, making breeding and selecting processes more difficult. Here we carried out whole-genome sequencing of five American minks with Royal pastel (b/b) phenotypes originating from two distinct mink populations. We identified an insertion of endogenous retroviral element type 1 (ERV1) into the first intron of the gene encoding the HPS3 protein, which regulates the trafficking of tyrosinase-containing vesicles to maturing melanosomes. With Cas9-targeted nanopore sequencing, we reconstructed the full-length sequence of the 11.7 Kb ERV1 insertion and observed hypermethylation that spread to the HPS3 gene promoter region. These findings highlight the role of HPS3 in the formation of melanosomes and melanin, as well as the genetic process regulating the intensity and spectrum of hair colour. Moreover, in mink breeding projects, these data are also useful for tracking economically important fur qualities.

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来源期刊
Animal genetics
Animal genetics 生物-奶制品与动物科学
CiteScore
4.60
自引率
4.20%
发文量
115
审稿时长
5 months
期刊介绍: Animal Genetics reports frontline research on immunogenetics, molecular genetics and functional genomics of economically important and domesticated animals. Publications include the study of variability at gene and protein levels, mapping of genes, traits and QTLs, associations between genes and traits, genetic diversity, and characterization of gene or protein expression and control related to phenotypic or genetic variation. The journal publishes full-length articles, short communications and brief notes, as well as commissioned and submitted mini-reviews on issues of interest to Animal Genetics readers.
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