Chuyu Lin, Chenghao Lan, Xiaoxiao Li, Wei Xie, Fucheng Lin, Yan Liang, Zeng Tao
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YB2 and YB3 function redundantly and positively regulate plant resistance against the necrotrophic pathogen <i>Botrytis cinerea</i>, which are specially required for transcriptional activation of a set of JA-responsive genes following inoculation. Furthermore, YB2 and YB3 modulated their expression through direct occupancy and interaction with histone demethylase Ref6 to remove repressive histone modifications. Moreover, YB2 and YB3 physically interacted with JAZ repressors and negatively modulated their abundance, which in turn attenuated the inhibition of JAZ proteins on the transcription of JA-responsive genes, thereby activating JA response and promoting disease resistance. 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引用次数: 0
摘要
植物激素茉莉酸盐(JA)通过协调全基因组转录重编程来调节植物的生长和免疫。在静止阶段,JASMONATE-ZIM DOMAIN(JAZ)蛋白作为主要抑制因子,调节 JA 信号通路中 JA 响应基因的表达。然而,JA 反应基因对 JA 处理的去抑制机制仍不清楚。在这里,我们报告了两种核因子 Y 转录因子 NF-YB2 和 NF-YB3(以下简称 YB2 和 YB3)在拟南芥的这种去抑制中发挥的关键作用。YB2和YB3具有冗余功能,能积极调节植物对坏死性病原菌Botrytis cinerea的抗性。此外,YB2 和 YB3 通过直接占据和与组蛋白去甲基化酶 Ref6 相互作用来消除抑制性组蛋白修饰,从而调节它们的表达。此外,YB2 和 YB3 还与 JAZ 抑制因子发生了物理作用,并对其丰度进行了负调控,进而减弱了 JAZ 蛋白对 JA 响应基因转录的抑制作用,从而激活了 JA 响应,促进了抗病性。总之,我们的研究揭示了YB2和YB3通过正向调节JA响应基因的转录和负向调节JAZ蛋白的丰度在JA信号转导中的正向调节作用。
A pair of nuclear factor Y transcription factors act as positive regulators in jasmonate signaling and disease resistance in Arabidopsis
The plant hormone jasmonate (JA) regulates plant growth and immunity by orchestrating a genome-wide transcriptional reprogramming. In the resting stage, JASMONATE-ZIM DOMAIN (JAZ) proteins act as main repressors to regulate the expression of JA-responsive genes in the JA signaling pathway. However, the mechanisms underlying de-repression of JA-responsive genes in response to JA treatment remain elusive. Here, we report two nuclear factor Y transcription factors NF-YB2 and NF-YB3 (thereafter YB2 and YB3) play key roles in such de-repression in Arabidopsis. YB2 and YB3 function redundantly and positively regulate plant resistance against the necrotrophic pathogen Botrytis cinerea, which are specially required for transcriptional activation of a set of JA-responsive genes following inoculation. Furthermore, YB2 and YB3 modulated their expression through direct occupancy and interaction with histone demethylase Ref6 to remove repressive histone modifications. Moreover, YB2 and YB3 physically interacted with JAZ repressors and negatively modulated their abundance, which in turn attenuated the inhibition of JAZ proteins on the transcription of JA-responsive genes, thereby activating JA response and promoting disease resistance. Overall, our study reveals the positive regulators of YB2 and YB3 in JA signaling by positively regulating transcription of JA-responsive genes and negatively modulating the abundance of JAZ proteins.
期刊介绍:
Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.