以 NAC 转录因子 MNAC3 为中心的调控网络可负向调节水稻对稻瘟病的免疫力。

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Hui Wang, Yan Bi, Yuqing Yan, Xi Yuan, Yizhou Gao, Muhammad Noman, Dayong Li, Fengming Song
{"title":"以 NAC 转录因子 MNAC3 为中心的调控网络可负向调节水稻对稻瘟病的免疫力。","authors":"Hui Wang,&nbsp;Yan Bi,&nbsp;Yuqing Yan,&nbsp;Xi Yuan,&nbsp;Yizhou Gao,&nbsp;Muhammad Noman,&nbsp;Dayong Li,&nbsp;Fengming Song","doi":"10.1111/jipb.13727","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>NAC transcription factors (TFs) are pivotal in plant immunity against diverse pathogens. Here, we report the functional and regulatory network of MNAC3, a novel NAC TF, in rice immunity. MNAC3, a transcriptional activator, negatively modulates rice immunity against blast and bacterial leaf blight diseases and pathogen-associated molecular pattern (PAMP)-triggered immune responses. MNAC3 binds to a CACG <i>cis</i>-element and activates the transcription of immune-negative target genes <i>OsINO80</i>, <i>OsJAZ10</i>, and <i>OsJAZ11</i>. The negative function of MNAC3 in rice immunity depends on its transcription of downstream genes such as <i>OsINO80</i> and <i>OsJAZ10</i>. MNAC3 interacts with immunity-related OsPP2C41 (a protein phosphatase), ONAC066 (a NAC TF), and OsDjA6 (a DnaJ chaperone). ONAC066 and OsPP2C41 attenuate MNAC3 transcriptional activity, while OsDjA6 promotes it. Phosphorylation of MNAC3 at S163 is critical for its negative functions in rice immunity. OsPP2C41, which plays positive roles in rice blast resistance and chitin-triggered immune responses, dephosphorylates MNAC3, suppressing its transcriptional activity on the target genes <i>OsINO80</i>, <i>OsJAZ10</i>, and <i>OsJAZ11</i> and promoting the translocation of MNAC3 from nucleus to cytoplasm. These results establish a MNAC3-centered regulatory network in which OsPP2C41 dephosphorylates MNAC3, attenuating its transcriptional activity on downstream immune-negative target genes in rice. Together, these findings deepen our understanding of molecular mechanisms in rice immunity and offer a novel strategy for genetic improvement of rice disease resistance.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 9","pages":"2017-2041"},"PeriodicalIF":9.3000,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13727","citationCount":"0","resultStr":"{\"title\":\"A NAC transcription factor MNAC3-centered regulatory network negatively modulates rice immunity against blast disease\",\"authors\":\"Hui Wang,&nbsp;Yan Bi,&nbsp;Yuqing Yan,&nbsp;Xi Yuan,&nbsp;Yizhou Gao,&nbsp;Muhammad Noman,&nbsp;Dayong Li,&nbsp;Fengming Song\",\"doi\":\"10.1111/jipb.13727\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>NAC transcription factors (TFs) are pivotal in plant immunity against diverse pathogens. Here, we report the functional and regulatory network of MNAC3, a novel NAC TF, in rice immunity. MNAC3, a transcriptional activator, negatively modulates rice immunity against blast and bacterial leaf blight diseases and pathogen-associated molecular pattern (PAMP)-triggered immune responses. MNAC3 binds to a CACG <i>cis</i>-element and activates the transcription of immune-negative target genes <i>OsINO80</i>, <i>OsJAZ10</i>, and <i>OsJAZ11</i>. The negative function of MNAC3 in rice immunity depends on its transcription of downstream genes such as <i>OsINO80</i> and <i>OsJAZ10</i>. MNAC3 interacts with immunity-related OsPP2C41 (a protein phosphatase), ONAC066 (a NAC TF), and OsDjA6 (a DnaJ chaperone). ONAC066 and OsPP2C41 attenuate MNAC3 transcriptional activity, while OsDjA6 promotes it. Phosphorylation of MNAC3 at S163 is critical for its negative functions in rice immunity. OsPP2C41, which plays positive roles in rice blast resistance and chitin-triggered immune responses, dephosphorylates MNAC3, suppressing its transcriptional activity on the target genes <i>OsINO80</i>, <i>OsJAZ10</i>, and <i>OsJAZ11</i> and promoting the translocation of MNAC3 from nucleus to cytoplasm. These results establish a MNAC3-centered regulatory network in which OsPP2C41 dephosphorylates MNAC3, attenuating its transcriptional activity on downstream immune-negative target genes in rice. Together, these findings deepen our understanding of molecular mechanisms in rice immunity and offer a novel strategy for genetic improvement of rice disease resistance.</p></div>\",\"PeriodicalId\":195,\"journal\":{\"name\":\"Journal of Integrative Plant Biology\",\"volume\":\"66 9\",\"pages\":\"2017-2041\"},\"PeriodicalIF\":9.3000,\"publicationDate\":\"2024-07-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13727\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Integrative Plant Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jipb.13727\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Integrative Plant Biology","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jipb.13727","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

NAC转录因子(TFs)在植物抵抗各种病原体的免疫中起着关键作用。在此,我们报告了新型 NAC TF MNAC3 在水稻免疫中的功能和调控网络。MNAC3 是一种转录激活因子,它能负向调节水稻对稻瘟病和细菌性叶枯病的免疫以及病原体相关分子模式(PAMP)触发的免疫反应。MNAC3 与 CACG 顺式元件结合,激活免疫负性靶基因 OsINO80、OsJAZ10 和 OsJAZ11 的转录。MNAC3 在水稻免疫中的负功能取决于它对 OsINO80 和 OsJAZ10 等下游基因的转录。MNAC3 与免疫相关的 OsPP2C41(一种蛋白磷酸酶)、ONAC066(一种 NAC TF)和 OsDjA6(一种 DnaJ 合子)相互作用。ONAC066 和 OsPP2C41 会削弱 MNAC3 的转录活性,而 OsDjA6 则会促进其转录活性。MNAC3 在 S163 处的磷酸化对其在水稻免疫中的负功能至关重要。OsPP2C41 在水稻抗稻瘟病和几丁质触发的免疫反应中发挥积极作用,它能使 MNAC3 去磷酸化,抑制其在靶基因 OsINO80、OsJAZ10 和 OsJAZ11 上的转录活性,并促进 MNAC3 从细胞核向细胞质的转位。这些结果建立了一个以 MNAC3 为中心的调控网络,其中 OsPP2C41 可使 MNAC3 去磷酸化,从而削弱其对水稻下游免疫阴性靶基因的转录活性。这些发现加深了我们对水稻免疫分子机制的理解,并为遗传改良水稻抗病性提供了一种新策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A NAC transcription factor MNAC3-centered regulatory network negatively modulates rice immunity against blast disease

A NAC transcription factor MNAC3-centered regulatory network negatively modulates rice immunity against blast disease

NAC transcription factors (TFs) are pivotal in plant immunity against diverse pathogens. Here, we report the functional and regulatory network of MNAC3, a novel NAC TF, in rice immunity. MNAC3, a transcriptional activator, negatively modulates rice immunity against blast and bacterial leaf blight diseases and pathogen-associated molecular pattern (PAMP)-triggered immune responses. MNAC3 binds to a CACG cis-element and activates the transcription of immune-negative target genes OsINO80, OsJAZ10, and OsJAZ11. The negative function of MNAC3 in rice immunity depends on its transcription of downstream genes such as OsINO80 and OsJAZ10. MNAC3 interacts with immunity-related OsPP2C41 (a protein phosphatase), ONAC066 (a NAC TF), and OsDjA6 (a DnaJ chaperone). ONAC066 and OsPP2C41 attenuate MNAC3 transcriptional activity, while OsDjA6 promotes it. Phosphorylation of MNAC3 at S163 is critical for its negative functions in rice immunity. OsPP2C41, which plays positive roles in rice blast resistance and chitin-triggered immune responses, dephosphorylates MNAC3, suppressing its transcriptional activity on the target genes OsINO80, OsJAZ10, and OsJAZ11 and promoting the translocation of MNAC3 from nucleus to cytoplasm. These results establish a MNAC3-centered regulatory network in which OsPP2C41 dephosphorylates MNAC3, attenuating its transcriptional activity on downstream immune-negative target genes in rice. Together, these findings deepen our understanding of molecular mechanisms in rice immunity and offer a novel strategy for genetic improvement of rice disease resistance.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信