基于元基因组分析的太湖沉积物中起主导作用的兼氧菌证据

IF 4.1 2区 环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Junming Ren , Sichuan Zhao , Lu Xu , Wenming Xie , Han Meng , Huan He , Limin Zhang
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引用次数: 0

摘要

硝化过程在氮循环中发挥着重要作用,其中由微生物介导的氨氧化过程是氮循环的限速步骤。环境因素会影响氨氧化微生物(AOM)的分布和活性,包括氨氧化细菌(AOB)、氨氧化古细菌(AOA)和完全氨氧化酶(comammox Nitrospira)。目前,大多数研究都以amoA作为氨氧化过程的标记基因,来分析环境中AOM群落组成和丰度的差异。本研究利用元基因组测序技术研究了不同富营养化水平下太湖沉积物中硝化微生物群落组成和功能基因分布的差异。结果发现,与AOB和AOA相比,属于Nitrospirota的comammox Nitrospira和典型亚硝酸盐氧化剂NOB Nitrospira在大多数地点的相对丰度较高。此外,氮循环相关基因的网络分析表明,氮代谢微生物的主要生存模式是互作。此外,通过元基因组分选重建了太湖沉积物中的微生物基因组,结果表明,在获得的167个分选中,有2个分选(分选9和分选32)被注释为复合氧化硝化螺菌,在以大型水生植物为主的湖泊(如南太湖和西岸)中具有较高的丰度。此外,属于 comammox 硝螺菌的第 9 仓注释了与氨氧化相关的 amoA 基因,以及与尿素分解和运输相关的其他基因,表明其功能具有多样性。总之,这些研究结果表明,AOM具有不同的分布特征,其中comammox Nitrospira具有较高的多样性,可能在以大型水生植物为主的湖泊中占主导地位。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evidence of comammox bacteria playing a dominant role in Lake Taihu sediments based on metagenomic analysis

The nitrification process plays an important role in the nitrogen cycle, in which the ammonia-oxidation process mediated by microorganisms is the rate-limiting step. Environmental factors can affect the distribution and activity of ammonia-oxidizing microorganisms (AOM), including ammonia-oxidizing bacteria (AOB), ammonia-oxidizing archaea (AOA), and complete ammonia oxidizer (comammox Nitrospira). At present, most studies have used amoA as a marker gene for the ammonia oxidation process to analyze the differences among AOM community composition and abundance in the environment. In this study, metagenomic sequencing was used to study the differences in community composition and functional gene distribution of nitrifying microorganisms in the sediments of Lake Taihu with different eutrophication levels. It was found that comammox Nitrospira and typical nitrite oxidizer NOB Nitrospira, which belong to Nitrospirota, had higher relative abundance at most sites compared to AOB and AOA. Furthermore, the network analysis of genes related to nitrogen cycle showed that the main survival mode of nitrogen metabolizing microorganisms was mutualism. Besides, the microbial genomes in the sediments of Lake Taihu were reconstructed by metagenomic binning, which showed that among the 167 bins obtained, 2 bins (bin 9 and bin 32) were annotated as comammox Nitrospira, and had high abundance in the macrophytes-dominated lakes (such as South Lake Taihu and West Coast). In addition, bin 9, which belongs to comammox Nitrospira, annotates amoA genes associated with ammonia oxidation, and other genes associated with urea decomposition and transport, suggesting functional diversity. Overall, these findings suggest that AOM have different distribution characteristics, among which comammox Nitrospira has high diversity and may be potentially dominant in macrophytes-dominated lakes.

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来源期刊
CiteScore
9.60
自引率
10.40%
发文量
107
审稿时长
21 days
期刊介绍: International Biodeterioration and Biodegradation publishes original research papers and reviews on the biological causes of deterioration or degradation.
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