{"title":"Manilkara zapota 碱溶性多糖-Fe(Ⅲ)复合物的制备、抗氧化活性和体外消化能力","authors":"Juan-li Fang, Fu-lan Hu, Tao Liu, Ying Liu, Peng-peng Sun, Yuan-yuan Ren","doi":"10.1016/j.procbio.2024.06.028","DOIUrl":null,"url":null,"abstract":"<div><p>In this study, alkali-soluble polysaccharide from <em>Manilkara zapota</em> (MZAP) was extracted and polysaccharide-iron complexes MZAP-Fe (III) was synthesized. Structural characterization, antioxidant and digestive properties were used to evaluate the feasibility of MZAP-Fe (III) as an iron supplement. The results showed that when the reaction temperature was 70 ℃, the pH was 9 and the addition of sodium citrate was 0.025 g, MZAP- Fe (Ⅲ) had the highest iron content (14.68 %) and its average molecular weight increased compared to MZAP. The UV and FT-IR results indicated that MZAP- Fe (Ⅲ) was formed by the carboxyl and hydroxyl groups in MZAP with the iron group to form Fe-O bonds. MZAP-Fe (III) maintained high thermal stability over the temperature range of 20°C to 450℃. When the concentration of MZAP- Fe (Ⅲ) was 1.0 mg/mL, hydroxyl radical-scavenging activity and DPPH radical scavenging activity reached 57.03 % and 82.21 %, respectively, and both were significantly higher than the antioxidant activity of MZAP. Moreover, in the simulating human digestion, MZAP- Fe (Ⅲ) was more stable than FeSO<sub>4</sub> and less likely to be destroyed by stomach acid. The present study demonstrated the potential of MZAP-Fe (III) as a novel iron supplement.</p></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Preparation, antioxidant activity and in vitro digestion of Manilkara zapota alkali-soluble polysaccharide-Fe (Ⅲ) complex\",\"authors\":\"Juan-li Fang, Fu-lan Hu, Tao Liu, Ying Liu, Peng-peng Sun, Yuan-yuan Ren\",\"doi\":\"10.1016/j.procbio.2024.06.028\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>In this study, alkali-soluble polysaccharide from <em>Manilkara zapota</em> (MZAP) was extracted and polysaccharide-iron complexes MZAP-Fe (III) was synthesized. Structural characterization, antioxidant and digestive properties were used to evaluate the feasibility of MZAP-Fe (III) as an iron supplement. The results showed that when the reaction temperature was 70 ℃, the pH was 9 and the addition of sodium citrate was 0.025 g, MZAP- Fe (Ⅲ) had the highest iron content (14.68 %) and its average molecular weight increased compared to MZAP. The UV and FT-IR results indicated that MZAP- Fe (Ⅲ) was formed by the carboxyl and hydroxyl groups in MZAP with the iron group to form Fe-O bonds. MZAP-Fe (III) maintained high thermal stability over the temperature range of 20°C to 450℃. When the concentration of MZAP- Fe (Ⅲ) was 1.0 mg/mL, hydroxyl radical-scavenging activity and DPPH radical scavenging activity reached 57.03 % and 82.21 %, respectively, and both were significantly higher than the antioxidant activity of MZAP. Moreover, in the simulating human digestion, MZAP- Fe (Ⅲ) was more stable than FeSO<sub>4</sub> and less likely to be destroyed by stomach acid. The present study demonstrated the potential of MZAP-Fe (III) as a novel iron supplement.</p></div>\",\"PeriodicalId\":20811,\"journal\":{\"name\":\"Process Biochemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-06-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Process Biochemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1359511324002149\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Process Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1359511324002149","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Preparation, antioxidant activity and in vitro digestion of Manilkara zapota alkali-soluble polysaccharide-Fe (Ⅲ) complex
In this study, alkali-soluble polysaccharide from Manilkara zapota (MZAP) was extracted and polysaccharide-iron complexes MZAP-Fe (III) was synthesized. Structural characterization, antioxidant and digestive properties were used to evaluate the feasibility of MZAP-Fe (III) as an iron supplement. The results showed that when the reaction temperature was 70 ℃, the pH was 9 and the addition of sodium citrate was 0.025 g, MZAP- Fe (Ⅲ) had the highest iron content (14.68 %) and its average molecular weight increased compared to MZAP. The UV and FT-IR results indicated that MZAP- Fe (Ⅲ) was formed by the carboxyl and hydroxyl groups in MZAP with the iron group to form Fe-O bonds. MZAP-Fe (III) maintained high thermal stability over the temperature range of 20°C to 450℃. When the concentration of MZAP- Fe (Ⅲ) was 1.0 mg/mL, hydroxyl radical-scavenging activity and DPPH radical scavenging activity reached 57.03 % and 82.21 %, respectively, and both were significantly higher than the antioxidant activity of MZAP. Moreover, in the simulating human digestion, MZAP- Fe (Ⅲ) was more stable than FeSO4 and less likely to be destroyed by stomach acid. The present study demonstrated the potential of MZAP-Fe (III) as a novel iron supplement.
期刊介绍:
Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.