纳秒光纤激光和药物电纺 PVA 涂层对 Ti-6Al-4 V 合金冶金和生物特性的协同效应

IF 7.5 Q1 CHEMISTRY, PHYSICAL
Tahmine Rajabi , Homam Naffakh-Moosavy , Fatemeh Bagheri
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引用次数: 0

摘要

如今,钛基植入物被广泛用于替代受损或缺失的人体器官。钛基植入物最常见的问题是与骨骼的化学结合力差,以及植入物表面形成生物膜导致感染。因此,抗菌性能和成骨细胞粘附性的改善是解决这些问题的目的。本研究的目的是利用表面粗糙度和原位抗生素药物释放来改善细胞粘附性和预防细菌感染。在这里,使用了沟距分别为 10、30 和 50 微米的微加工(纳秒级)激光进行表面改性。利用 X 射线衍射 (XRD)、场发射扫描电子显微镜 (FESEM)、硬度、粗糙度和润湿性测试对表面改性样品进行物理和冶金表征,以找到最佳的激光加工条件。粗糙度因激光表面改性而增加,合金的表面特性对沟槽距离很敏感。槽距越小,粗糙度和润湿性越高。在熔合区观察到马氏体相、α 相和 Ti3Al。此外,在熔合区和热影响区还出现了β相的溶解。没有观察到氧化现象。所有这些现象的发生都没有改变体积。然后,利用电纺丝工艺在优化的样品表面涂覆万古霉素负载的聚乙烯醇溶液,并对其毒性、细胞粘附性和药物释放率进行了评估。结果表明,激光表面改性和涂层不会损害细胞活力。与未改性样品相比,改性样品具有较高的细胞粘附性,药物释放率也有所提高。改性样品的药物释放时间从 4 小时延长到 25 小时。因此,改良后的植入物也能显示出抗生素的持续释放。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The synergic effect of nanosecond fiber laser and drug-loaded electrospun PVA coating on metallurgical and biological characteristics of Ti-6Al-4 V alloy

Nowadays, titanium-based implants are widely used to replace damaged or missing body organs. Poor chemical bonding with the bone and infection caused by formation of biofilm on the implant surface are the most common problems with them. So, antibacterial properties and osteoblast adhesion improvement have been intended to address these issues. The aim of this research is cell adhesion improvement and prevention of bacterial infection using surface roughness and in-situ antibiotic drug release. Here, micromachining (nanoseconds) laser with a groove distance of 10, 30, and 50 µm, was used to surface modification. X-ray diffraction (XRD), field emission scanning electron microscope (FESEM), hardness, roughness, and wettability tests were used for physical and metallurgical characterization of surface-modified samples to find the optimum laser processing conditions. Roughness has increased as a result of laser surface modification and surface characteristics of alloy exhibit sensitivity to the groove distance. The lower groove distance indicated the higher roughness and wettability. Martensite phase, α phase, and, Ti3Al were observed in the fusion zone. Also, the dissolution of the beta phase has occurred in the fusion and the heat-affected zones. No oxidation was observed. All these occurred without any change in bulk. Then optimized sample surfaces were coated by the vancomycin-loaded polyvinyl alcohol solution using electrospinning process, and toxicity, cell adhesion, and drug release rate were evaluated. The results showed laser surface modification and coating did not hurt cell viability. Modified samples demonstrated high cell adhesion and improvement in drug release compared to the unmodified samples. The drug release rate was extended from 4 h to 25 h for modified samples. So, the modified implants could indicate a sustained release of antibiotics as well.

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CiteScore
8.10
自引率
1.60%
发文量
128
审稿时长
66 days
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