Zhenyan Xie , Die Zhao , Bingxue Li , Nan Zeng , Dandan Wang , Xinxin Wang , Yunhao Sun , Mingwei Shao , Shuang Miao , Fei Feng , Ping Cheng , Guohui Yu , Chunji Li
{"title":"脂质体组和转录物组的综合分析有助于深入了解过氧化氢处理对油脂红酵母 Sporobolomyces pararoseus 脂质合成的积极影响","authors":"Zhenyan Xie , Die Zhao , Bingxue Li , Nan Zeng , Dandan Wang , Xinxin Wang , Yunhao Sun , Mingwei Shao , Shuang Miao , Fei Feng , Ping Cheng , Guohui Yu , Chunji Li","doi":"10.1016/j.lwt.2024.116389","DOIUrl":null,"url":null,"abstract":"<div><p>Microbial lipids are a group of promising renewable sources that are highly demanded by the food, feed, pharmaceutical, and biofuel industries. <em>Sporobolomyces pararoseus</em> is a well-studied oleaginous yeast that is usually considered superior for the industrial production of microbial lipids over other conventional microorganisms. The objective of this study was to investigate the specific effect of hydrogen peroxide treatment on the biosynthesis of microbial lipids in <em>S. pararoseus</em> via lipidomics and transcriptomics technologies. These results showed that hydrogen peroxide treatment significantly enhanced lipid production in <em>S. pararoseus</em> CJR, especially the triacylglycerols. The up-regulation of several key lipogenic genes might be one of the main reasons for this enhancement. Integrative analysis of the lipidome and transcriptome revealed that some key protein-encoding genes and transcription factors might play a crucial regulatory role in lipid biosynthesis under hydrogen peroxide stress. Together, the results presented herein not only provided a feasible fermentation process for boosting the yield of certain lipids but also deepened our understanding of the lipid biosynthesis in <em>S. pararoseus</em> CJR. This could serve as a molecular cornerstone for enhancing their yield through genetic engineering and then further facilitate commercial applications of microbial lipids synthesized by <em>S. pararoseus</em> in more industrial sectors.</p></div>","PeriodicalId":382,"journal":{"name":"LWT - Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":6.0000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0023643824006686/pdfft?md5=b0cd3185b04eff2cbda428fd45255849&pid=1-s2.0-S0023643824006686-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Integrated lipidomic and transcriptomic analyses provide insights into the positive effect of hydrogen peroxide treatment on lipid synthesis in oleaginous red yeast Sporobolomyces pararoseus\",\"authors\":\"Zhenyan Xie , Die Zhao , Bingxue Li , Nan Zeng , Dandan Wang , Xinxin Wang , Yunhao Sun , Mingwei Shao , Shuang Miao , Fei Feng , Ping Cheng , Guohui Yu , Chunji Li\",\"doi\":\"10.1016/j.lwt.2024.116389\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Microbial lipids are a group of promising renewable sources that are highly demanded by the food, feed, pharmaceutical, and biofuel industries. <em>Sporobolomyces pararoseus</em> is a well-studied oleaginous yeast that is usually considered superior for the industrial production of microbial lipids over other conventional microorganisms. The objective of this study was to investigate the specific effect of hydrogen peroxide treatment on the biosynthesis of microbial lipids in <em>S. pararoseus</em> via lipidomics and transcriptomics technologies. These results showed that hydrogen peroxide treatment significantly enhanced lipid production in <em>S. pararoseus</em> CJR, especially the triacylglycerols. The up-regulation of several key lipogenic genes might be one of the main reasons for this enhancement. Integrative analysis of the lipidome and transcriptome revealed that some key protein-encoding genes and transcription factors might play a crucial regulatory role in lipid biosynthesis under hydrogen peroxide stress. Together, the results presented herein not only provided a feasible fermentation process for boosting the yield of certain lipids but also deepened our understanding of the lipid biosynthesis in <em>S. pararoseus</em> CJR. This could serve as a molecular cornerstone for enhancing their yield through genetic engineering and then further facilitate commercial applications of microbial lipids synthesized by <em>S. pararoseus</em> in more industrial sectors.</p></div>\",\"PeriodicalId\":382,\"journal\":{\"name\":\"LWT - Food Science and Technology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.0000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0023643824006686/pdfft?md5=b0cd3185b04eff2cbda428fd45255849&pid=1-s2.0-S0023643824006686-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"LWT - Food Science and Technology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0023643824006686\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"LWT - Food Science and Technology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0023643824006686","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
微生物脂类是一类前景广阔的可再生资源,在食品、饲料、制药和生物燃料工业中需求量很大。副嗜油孢子酵母是一种已被充分研究的含油酵母,通常被认为在微生物脂类的工业生产中优于其他传统微生物。本研究的目的是通过脂质组学和转录组学技术研究过氧化氢处理对副嗜酸梭菌微生物脂质生物合成的具体影响。结果表明,过氧化氢处理显著提高了副猪痢疾杆菌 CJR 的脂质产量,尤其是三酰甘油。几个关键生脂基因的上调可能是导致这种提高的主要原因之一。对脂质体和转录组的综合分析表明,一些关键的蛋白编码基因和转录因子可能在过氧化氢胁迫下的脂质生物合成过程中发挥着重要的调控作用。总之,本文介绍的结果不仅为提高某些脂质的产量提供了可行的发酵工艺,而且加深了我们对 S. pararoseus CJR 脂质生物合成的理解。这可以作为通过基因工程提高其产量的分子基石,进而进一步促进由 S. pararoseus 合成的微生物脂类在更多工业领域的商业应用。
Integrated lipidomic and transcriptomic analyses provide insights into the positive effect of hydrogen peroxide treatment on lipid synthesis in oleaginous red yeast Sporobolomyces pararoseus
Microbial lipids are a group of promising renewable sources that are highly demanded by the food, feed, pharmaceutical, and biofuel industries. Sporobolomyces pararoseus is a well-studied oleaginous yeast that is usually considered superior for the industrial production of microbial lipids over other conventional microorganisms. The objective of this study was to investigate the specific effect of hydrogen peroxide treatment on the biosynthesis of microbial lipids in S. pararoseus via lipidomics and transcriptomics technologies. These results showed that hydrogen peroxide treatment significantly enhanced lipid production in S. pararoseus CJR, especially the triacylglycerols. The up-regulation of several key lipogenic genes might be one of the main reasons for this enhancement. Integrative analysis of the lipidome and transcriptome revealed that some key protein-encoding genes and transcription factors might play a crucial regulatory role in lipid biosynthesis under hydrogen peroxide stress. Together, the results presented herein not only provided a feasible fermentation process for boosting the yield of certain lipids but also deepened our understanding of the lipid biosynthesis in S. pararoseus CJR. This could serve as a molecular cornerstone for enhancing their yield through genetic engineering and then further facilitate commercial applications of microbial lipids synthesized by S. pararoseus in more industrial sectors.
期刊介绍:
LWT - Food Science and Technology is an international journal that publishes innovative papers in the fields of food chemistry, biochemistry, microbiology, technology and nutrition. The work described should be innovative either in the approach or in the methods used. The significance of the results either for the science community or for the food industry must also be specified. Contributions written in English are welcomed in the form of review articles, short reviews, research papers, and research notes. Papers featuring animal trials and cell cultures are outside the scope of the journal and will not be considered for publication.