普通和纳米级双孢蘑菇提取物对玉米粒中黄曲霉菌菌丝生长和黄曲霉毒素产生的抑制效率评价

IF 2.7 2区 农林科学 Q1 ENTOMOLOGY
Falah Abdul Hassan Hussein, Osama Abdul-Rahman Owied
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引用次数: 0

摘要

这项研究旨在利用姬松茸属植物的普通提取物和纳米级提取物保护玉米作物免受黄曲霉菌(A. flavus)黄曲霉毒素 B1(AFB1)的感染和产生。 HPLC 技术表明,从穆萨伊布地区分离的黄曲霉菌(AFA5)是毒素产生量最高的菌株。AFB1 的浓度为 77.02 ppb。PCR 产物显示,根据所使用的引物,ITS1 的 ITS4- 区域扩增成功,出现了一个大小为 744 bp(碱基对)的单束。从不同地点采集的玉米样本的分离和诊断结果显示,黄曲霉和不同属的真菌(即曲霉属、镰刀菌属、青霉属和根霉属)都存在,由曲霉属发出的频率分别为 18.33%、38.87%、20.19%、15.92% 和 6.69%。在马铃薯葡萄糖琼脂(PDA)上,双孢蘑菇(A. bisporus)提取物对黄曲霉的生长有抑制作用,普通提取物的抑制率分别为 43.95%、34.59%和 10.90%,纳米提取物的抑制率分别为 18.74%、43.71%和 76.56%,浓度分别为 250、500 和 100 ppm。在酵母提取物蔗糖肉汤(YES)培养基中添加双孢蘑菇提取物(普通提取物和纳米提取物)后,AFB1 的产量分别减少了 66% 和 72.14%。总之,我们的研究为未来的科学研究提供了一个新的视角,即利用纳米技术从自然资源中生产生物配料的可能性,该技术有能力安全、环保地控制农业生产(尤其是玉米谷物)中霉菌毒素的产生,并鼓励广泛的商业应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Efficiency evaluation of ordinary and nanoscale extracts of Agaricus bisporus. on inhibition mycelial growth and aflatoxin production of Aspergillus flavus in maize grain

This study aimed to protect maize crops from infection and production of Aspergillus flavus (A. flavus) aflatoxin B1(AFB1), using the ordinary and nanoscale extracts of Agaricus spp. HPLC technology showed that the isolate taken from Musayyib district which coded with (AFA5) was a highest toxin producer. AFB1 at a concentration of 77.02 ppb. PCR product showed that the amplification of the ITS4- region was successful ITS1 according to the primers used, where a single bundle with a size of 744 bp (base pair) appeared. The isolated and diagnostic results of maize samples collected from separate sites showed the presence of A. flavus and different genera of fungi, namely Aspergillus spp., Fusarium spp., Penicillium spp., and Rhizopus spp., issued by the genus Aspergillus spp. with frequency rates of 18.33, 38.87, 20.19, 15.92, and 6.69 % respectively. On Potato Dextrose Agar (PDA), inhibitory effect results of Agaricus bisporus (A. bisporus) extract in inhibiting A. flavus growth, with inhibition percentages for the ordinary extract of 43.95, 34.59, and 10.90%, respectively, and for the nano extract 18.74, 43.71, and 76.56 % at 250,500 and 100 ppm respectively. AFB1 production was reduced by adding A. bisporus extracts (ordinary and nano) to Yeast Extract Sucrose Broth (YES) medium by 66% and 72.14%, respectively. Overall, our study presented a new insight for future scientific researches possibility of biocompands production using nanotechnology from natural resources that have the ability to safely environmentally control on mycotoxins production that present in agricultural production especially maize grains and encourage to widespread commercially use.

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来源期刊
CiteScore
5.70
自引率
18.50%
发文量
112
审稿时长
45 days
期刊介绍: The Journal of Stored Products Research provides an international medium for the publication of both reviews and original results from laboratory and field studies on the preservation and safety of stored products, notably food stocks, covering storage-related problems from the producer through the supply chain to the consumer. Stored products are characterised by having relatively low moisture content and include raw and semi-processed foods, animal feedstuffs, and a range of other durable items, including materials such as clothing or museum artefacts.
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