Sonia Garcia Mendez , Stephanie Fordeyn , Caroline De Tender , Sofie Goormachtig , Anne Willems
{"title":"季节会影响野生蒲葵种群的根部微生物群吗?","authors":"Sonia Garcia Mendez , Stephanie Fordeyn , Caroline De Tender , Sofie Goormachtig , Anne Willems","doi":"10.1016/j.rhisph.2024.100929","DOIUrl":null,"url":null,"abstract":"<div><p>Low temperatures, one of the main characteristics of winters in temperate climates, are restrictive for agriculture, because they negatively affect plant growth and development. To alleviate cold stress, plants are able to establish a symbiosis with soil bacteria. Annual bluegrass (<em>Poa annua</em>) is adapted to low temperatures, has a global distribution, and colonizes a wide range of environments, including polar regions. Here, <em>Poa annua</em> plants were harvested randomly within a one square meter plot at five sites in Flanders, Belgium, during September (summer samples) and March (winter samples) over 2019 and 2020. For each time point, location and season, ten samples of bulk soil, rhizosphere soil and root endosphere were obtained, making a total of 570 samples. As specialized bacteria in the winter root microbiome may reduce the negative impact of cold, we wanted to unravel how winter temperatures affect the root microbiome. Amplicon sequencing of 16S rRNA genes revealed that location was the factor with the largest influence on the bacterial community composition, followed by compartment (root endosphere, rhizosphere, and bulk soil), season, and year. While season was not the main determining factor of the root endosphere, comparison of the summer and winter samples allowed the identification of cold-enriched root-colonizing bacterial families. Interestingly, one ASV corresponding to <em>Massilia</em> was enriched in all sites during the winter season and, thus, may represent an interesting taxonomic group for the development of biostimulants to help plants cope with cold conditions.</p></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"31 ","pages":"Article 100929"},"PeriodicalIF":3.4000,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2452219824000843/pdfft?md5=22f5e5187515f085ab53c0a7cb8da6a9&pid=1-s2.0-S2452219824000843-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Do seasons impact the root microbiome of wild Poa annua populations?\",\"authors\":\"Sonia Garcia Mendez , Stephanie Fordeyn , Caroline De Tender , Sofie Goormachtig , Anne Willems\",\"doi\":\"10.1016/j.rhisph.2024.100929\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Low temperatures, one of the main characteristics of winters in temperate climates, are restrictive for agriculture, because they negatively affect plant growth and development. To alleviate cold stress, plants are able to establish a symbiosis with soil bacteria. Annual bluegrass (<em>Poa annua</em>) is adapted to low temperatures, has a global distribution, and colonizes a wide range of environments, including polar regions. Here, <em>Poa annua</em> plants were harvested randomly within a one square meter plot at five sites in Flanders, Belgium, during September (summer samples) and March (winter samples) over 2019 and 2020. For each time point, location and season, ten samples of bulk soil, rhizosphere soil and root endosphere were obtained, making a total of 570 samples. As specialized bacteria in the winter root microbiome may reduce the negative impact of cold, we wanted to unravel how winter temperatures affect the root microbiome. Amplicon sequencing of 16S rRNA genes revealed that location was the factor with the largest influence on the bacterial community composition, followed by compartment (root endosphere, rhizosphere, and bulk soil), season, and year. While season was not the main determining factor of the root endosphere, comparison of the summer and winter samples allowed the identification of cold-enriched root-colonizing bacterial families. Interestingly, one ASV corresponding to <em>Massilia</em> was enriched in all sites during the winter season and, thus, may represent an interesting taxonomic group for the development of biostimulants to help plants cope with cold conditions.</p></div>\",\"PeriodicalId\":48589,\"journal\":{\"name\":\"Rhizosphere\",\"volume\":\"31 \",\"pages\":\"Article 100929\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-06-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2452219824000843/pdfft?md5=22f5e5187515f085ab53c0a7cb8da6a9&pid=1-s2.0-S2452219824000843-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Rhizosphere\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2452219824000843\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rhizosphere","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2452219824000843","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Do seasons impact the root microbiome of wild Poa annua populations?
Low temperatures, one of the main characteristics of winters in temperate climates, are restrictive for agriculture, because they negatively affect plant growth and development. To alleviate cold stress, plants are able to establish a symbiosis with soil bacteria. Annual bluegrass (Poa annua) is adapted to low temperatures, has a global distribution, and colonizes a wide range of environments, including polar regions. Here, Poa annua plants were harvested randomly within a one square meter plot at five sites in Flanders, Belgium, during September (summer samples) and March (winter samples) over 2019 and 2020. For each time point, location and season, ten samples of bulk soil, rhizosphere soil and root endosphere were obtained, making a total of 570 samples. As specialized bacteria in the winter root microbiome may reduce the negative impact of cold, we wanted to unravel how winter temperatures affect the root microbiome. Amplicon sequencing of 16S rRNA genes revealed that location was the factor with the largest influence on the bacterial community composition, followed by compartment (root endosphere, rhizosphere, and bulk soil), season, and year. While season was not the main determining factor of the root endosphere, comparison of the summer and winter samples allowed the identification of cold-enriched root-colonizing bacterial families. Interestingly, one ASV corresponding to Massilia was enriched in all sites during the winter season and, thus, may represent an interesting taxonomic group for the development of biostimulants to help plants cope with cold conditions.
RhizosphereAgricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
5.70
自引率
8.10%
发文量
155
审稿时长
29 days
期刊介绍:
Rhizosphere aims to advance the frontier of our understanding of plant-soil interactions. Rhizosphere is a multidisciplinary journal that publishes research on the interactions between plant roots, soil organisms, nutrients, and water. Except carbon fixation by photosynthesis, plants obtain all other elements primarily from soil through roots.
We are beginning to understand how communications at the rhizosphere, with soil organisms and other plant species, affect root exudates and nutrient uptake. This rapidly evolving subject utilizes molecular biology and genomic tools, food web or community structure manipulations, high performance liquid chromatography, isotopic analysis, diverse spectroscopic analytics, tomography and other microscopy, complex statistical and modeling tools.