快速 MAS 机制下 α-突触核蛋白淀粉样纤维的固态 NMR 主干化学位移分配。

IF 0.8 4区 生物学 Q4 BIOPHYSICS
Zigmantas Toleikis, Piotr Paluch, Ewelina Kuc, Jana Petkus, Darius Sulskis, Mai-Liis Org-Tago, Ago Samoson, Vytautas Smirnovas, Jan Stanek, Alons Lends
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引用次数: 0

摘要

α-突触核蛋白(α-syn)淀粉样纤维与多种神经退行性疾病有关。固态核磁共振(ssNMR)已被证明是研究α-syn聚集体的有力工具。在此,我们报告了在快速(95 kHz)魔角旋光条件下使用质子检测的 ssNMR 光谱获得的一种新的α-syn 多晶体的 1H、13C 和 15N 骨背化学位移。使用 FLYA 算法对手动化学位移分配进行了交叉验证。利用 13C 化学位移差异和 TALOS 软件计算了 α-syn 纤维的二级结构元素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Solid-state NMR backbone chemical shift assignments of α-synuclein amyloid fibrils at fast MAS regime

Solid-state NMR backbone chemical shift assignments of α-synuclein amyloid fibrils at fast MAS regime

Solid-state NMR backbone chemical shift assignments of α-synuclein amyloid fibrils at fast MAS regime

The α-synuclein (α-syn) amyloid fibrils are involved in various neurogenerative diseases. Solid-state NMR (ssNMR) has been showed as a powerful tool to study α-syn aggregates. Here, we report the 1H, 13C and 15N back-bone chemical shifts of a new α-syn polymorph obtained using proton-detected ssNMR spectroscopy under fast (95 kHz) magic-angle spinning conditions. The manual chemical shift assignments were cross-validated using FLYA algorithm. The secondary structural elements of α-syn fibrils were calculated using 13C chemical shift differences and TALOS software.

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来源期刊
Biomolecular NMR Assignments
Biomolecular NMR Assignments 生物-光谱学
CiteScore
1.70
自引率
11.10%
发文量
59
审稿时长
6-12 weeks
期刊介绍: Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.
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