Chethanakumar, Mahantesh B. Budri, Kalagouda B. Gudasi, Ramesh S. Vadavi, Mallikarjun K. Patil, Vijay M. Kumbar and Sanjeev R. Inamdar
{"title":"用于快速识别锌(II)的三臂希夫碱荧光传感器:在活细胞成像、试纸和 TLC 中的应用。","authors":"Chethanakumar, Mahantesh B. Budri, Kalagouda B. Gudasi, Ramesh S. Vadavi, Mallikarjun K. Patil, Vijay M. Kumbar and Sanjeev R. Inamdar","doi":"10.1039/D4AY00774C","DOIUrl":null,"url":null,"abstract":"<p >Various metal ions exist in nature and human beings and play limitless vital roles in both the atmosphere and biology. A fundamental and useful aspect is the qualitative and quantitative assessment of Zn(<small>II</small>) at concentration levels as low as parts per billion (ppb). Thus, the design and development of novel fluorescent turn-on receptors have gained significant interest because of their potential for use in live cell imaging to detect biologically relevant metal ions with high selectivity and sensitivity. The present research illustrates the design and synthesis of a novel fluorescent sensor [(1,3,5-triazine-2,4,6-triyl)tris(hydrazine-2-yl-1-ylidene)tris(methaneylylidene)]tris(2,4-di-<em>tert</em>-butylphenol) (THDBP) for the selective and sensitive probing of Zn(<small>II</small>). The sensor exhibited a fluorescence turn-on mechanism upon treatment with Zn(<small>II</small>) ions at <em>λ</em><small><sub>emi.</sub></small> 503 nm in aq. acetonitrile. The formation of a 1 : 3 complex between THDBP and Zn(<small>II</small>) is confirmed from the Job plot and ESI-MS spectrum. The evaluated limit of detection (LOD) and association constant (<em>K</em><small><sub>a</sub></small>) of the sensor THDBP for Zn(<small>II</small>) were found to be 1.03 × 10<small><sup>−10</sup></small> M and 2.33 × 10<small><sup>8</sup></small> M<small><sup>−1</sup></small>, respectively. Further research demonstrates the practical application of the sensor for the detection of Zn(<small>II</small>) ions in live cells. The sensing ability of the sensor THDBP was also explored through inexpensive test strips and TLC sheets.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Tri-armed Schiff base fluorescent sensor for the rapid recognition of Zn(ii): application in live cell imaging, test strips and TLC†\",\"authors\":\"Chethanakumar, Mahantesh B. Budri, Kalagouda B. Gudasi, Ramesh S. Vadavi, Mallikarjun K. Patil, Vijay M. Kumbar and Sanjeev R. Inamdar\",\"doi\":\"10.1039/D4AY00774C\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Various metal ions exist in nature and human beings and play limitless vital roles in both the atmosphere and biology. A fundamental and useful aspect is the qualitative and quantitative assessment of Zn(<small>II</small>) at concentration levels as low as parts per billion (ppb). Thus, the design and development of novel fluorescent turn-on receptors have gained significant interest because of their potential for use in live cell imaging to detect biologically relevant metal ions with high selectivity and sensitivity. The present research illustrates the design and synthesis of a novel fluorescent sensor [(1,3,5-triazine-2,4,6-triyl)tris(hydrazine-2-yl-1-ylidene)tris(methaneylylidene)]tris(2,4-di-<em>tert</em>-butylphenol) (THDBP) for the selective and sensitive probing of Zn(<small>II</small>). The sensor exhibited a fluorescence turn-on mechanism upon treatment with Zn(<small>II</small>) ions at <em>λ</em><small><sub>emi.</sub></small> 503 nm in aq. acetonitrile. The formation of a 1 : 3 complex between THDBP and Zn(<small>II</small>) is confirmed from the Job plot and ESI-MS spectrum. The evaluated limit of detection (LOD) and association constant (<em>K</em><small><sub>a</sub></small>) of the sensor THDBP for Zn(<small>II</small>) were found to be 1.03 × 10<small><sup>−10</sup></small> M and 2.33 × 10<small><sup>8</sup></small> M<small><sup>−1</sup></small>, respectively. Further research demonstrates the practical application of the sensor for the detection of Zn(<small>II</small>) ions in live cells. The sensing ability of the sensor THDBP was also explored through inexpensive test strips and TLC sheets.</p>\",\"PeriodicalId\":64,\"journal\":{\"name\":\"Analytical Methods\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-06-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Methods\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2024/ay/d4ay00774c\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Methods","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/ay/d4ay00774c","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Tri-armed Schiff base fluorescent sensor for the rapid recognition of Zn(ii): application in live cell imaging, test strips and TLC†
Various metal ions exist in nature and human beings and play limitless vital roles in both the atmosphere and biology. A fundamental and useful aspect is the qualitative and quantitative assessment of Zn(II) at concentration levels as low as parts per billion (ppb). Thus, the design and development of novel fluorescent turn-on receptors have gained significant interest because of their potential for use in live cell imaging to detect biologically relevant metal ions with high selectivity and sensitivity. The present research illustrates the design and synthesis of a novel fluorescent sensor [(1,3,5-triazine-2,4,6-triyl)tris(hydrazine-2-yl-1-ylidene)tris(methaneylylidene)]tris(2,4-di-tert-butylphenol) (THDBP) for the selective and sensitive probing of Zn(II). The sensor exhibited a fluorescence turn-on mechanism upon treatment with Zn(II) ions at λemi. 503 nm in aq. acetonitrile. The formation of a 1 : 3 complex between THDBP and Zn(II) is confirmed from the Job plot and ESI-MS spectrum. The evaluated limit of detection (LOD) and association constant (Ka) of the sensor THDBP for Zn(II) were found to be 1.03 × 10−10 M and 2.33 × 108 M−1, respectively. Further research demonstrates the practical application of the sensor for the detection of Zn(II) ions in live cells. The sensing ability of the sensor THDBP was also explored through inexpensive test strips and TLC sheets.