甲醇中的黑胡椒籽提取物对革兰氏阳性金黄色葡萄球菌和革兰氏阴性绿脓杆菌的影响

Mymensingh medical journal : MMJ Pub Date : 2024-07-01
M A Hossain, S M Ahmed, N Haque, K E Zannat, S M Tanzim, A Afrin, T A Banu, T S Dema
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引用次数: 0

摘要

由于滥用和监管问题,有害微生物对多种药物产生抗药性,抗生素的作用受到威胁。新出现的微生物、抗药性机制和抗菌药物都需要进行广泛的研究。评估从黑胡椒种子(Piper nigrum L.)中分离出的甲醇提取物对两种感染病原体(革兰氏阳性金黄色葡萄球菌和革兰氏阴性绿脓杆菌)的体外抗菌活性。这项实验研究于 2022 年 7 月至 2023 年 6 月在迈门辛医学院药理学和治疗学系与微生物学系联合进行。提取物的溶剂为甲醇和 10.0%的二甲基亚砜(DMSO)。使用盘扩散法和肉汤稀释法评估了不同剂量的黑胡椒籽甲醇提取物(MBPE)的抗菌活性。在肉汤稀释法中,使用了传统抗生素环丙沙星,并将结果与甲醇提取物的结果进行了对比。使用了七种不同浓度(100、80、60、40、20、10 和 5 毫克/毫升)的黑胡椒籽甲醇提取物(MBPE),然后根据需要选择浓度,以确认提取物更精确的抗菌敏感性范围。80 毫克/毫升及以上剂量的甲基溴萃取物对上述微生物具有抑制作用。金黄色葡萄球菌和铜绿假单胞菌在 MBPE 中的 MIC 分别为 60 和 70 毫克/毫升。环丙沙星对金黄色葡萄球菌的 MIC 值为 1μg/ml ,对铜绿假单胞菌的 MIC 值为 1.5μg/ml。与 MBPE 对受试微生物的 MIC 值相比,环丙沙星的 MIC 值最低。这项研究清楚地表明,金黄色葡萄球菌和绿脓杆菌对黑胡椒籽甲醇提取物的抗菌特性很敏感。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Influence of Black pepper (Piper nigrum L) Seed Extract in Methanol against Gram Positive Staphylococcus aureus and Gram-Negative Pseudomonas aeruginosa.

Antibiotics' usefulness is threatened by multi-drugs resistance in harmful microorganisms because of abuse and regulatory problems. Emerging microbes, resistance mechanisms and antimicrobial drugs all require extensive investigation. Evaluation of the in vitro antibacterial activity of Methanolic extracts isolated from Black pepper seeds (Piper nigrum L.) against two infection causing pathogens, Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa. From July 2022 and June 2023, this experimental study was conducted at the Mymensingh Medical College's Department of Pharmacology and Therapeutics in conjunction with the Department of Microbiology. The solvents Methanol and 10.0% Di-Methyl Sulfoxide (DMSO) were used to make the extract. Using the disc diffusion and broth dilution methods, the antibacterial activity of methanolic extract of black pepper seeds (MBPE) was evaluated at various doses. Using the broth dilution procedure, the conventional antibiotic Ciprofloxacin was utilized, and the outcome was contrasted with that of Methanol extracts. Methanolic extract of black pepper seeds (MBPE) at seven distinct concentrations (100, 80, 60, 40, 20, 10 and 5mg/ml) were utilized, then later in chosen concentrations as needed to confirm the extracts' more precise margin of antimicrobial sensitivity. At 80mg/ml and above doses of the MBPE, it had an inhibitory impact against the aforementioned microorganisms. For Staphylococcus aureus and Pseudomonas aeruginosa the MIC were 60 and 70mg/ml in MBPE respectively. As of the MIC of Ciprofloxacin was 1μg/ml against Staphylococcus aureus and 1.5μg/ml for Pseudomonas aeruginosa. In comparison to MICs of MBPE for the test organisms, the MIC of Ciprofloxacin was the lowest. This study clearly shows that Staphylococcus aureus and Pseudomonas aeruginosa are sensitive to the methanolic extract of black pepper seeds' antibacterial properties.

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