对补充了黄斑色素类胡萝卜素的青光眼患者血清进行拉曼光谱分析。

IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS
Joy Udensi, Ekaterina Loskutova, James Loughman, Hugh J. Byrne
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引用次数: 0

摘要

由于血液中含有膳食中所有主要的类胡萝卜素,因此血液是评估其体内含量的合适基质。在欧洲青光眼管理营养试验中,开角型青光眼患者服用了 18 个月的黄斑色素类胡萝卜素(叶黄素、玉米黄质和中玉米黄质)补充剂后,对补充前后参与者血清中类胡萝卜素的含量进行了拉曼光谱分析,以研究补充方案对全身的影响,并探索一种利用常规血液检测量化这种影响的更直接方法。使用 532 nm 激光光源以获得最佳响应,在补充血清中观察到类胡萝卜素浓度持续增加,在最初类胡萝卜素基线含量较高的患者中增幅最大。1519 cm-1 类胡萝卜素峰的移动也显示了两组患者类胡萝卜素结构的差异。研究结果凸显了拉曼光谱直接量化和区分血清中类胡萝卜素的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Raman spectroscopic analysis of human blood serum of glaucoma patients supplemented with macular pigment carotenoids

Raman spectroscopic analysis of human blood serum of glaucoma patients supplemented with macular pigment carotenoids

As all major dietary carotenoids are contained in blood, it is a suitable substrate to evaluate their content, in vivo. Following 18-month supplementation of open-angle glaucoma patients with macula-pigment carotenoids (Lutein, Zeaxanthin and Meso-Zeaxanthin) in the European Nutrition in Glaucoma Management trial, Raman spectroscopic analysis of the carotenoid content of pre- and post-supplementation participant blood serum was carried out, to investigate the systemic impact of the supplementation regimen and explore a more direct way of quantifying this impact using routine blood tests. Using a 532 nm laser source for optimal response, a consistent increase in serum carotenoid concentration was observed in the supplemented serum, highest in patients with initial high baseline carotenoid content. A shift in the 1519 cm−1 carotenoid peak also revealed differences in the carotenoid structural profile of the two groups. The findings highlight the potential of Raman spectroscopy toquantify and differentiate carotenoids directly in blood serum.

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来源期刊
Journal of Biophotonics
Journal of Biophotonics 生物-生化研究方法
CiteScore
5.70
自引率
7.10%
发文量
248
审稿时长
1 months
期刊介绍: The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.
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