抑制补体可减少老年大鼠脑室内出血后的早期红细胞溶解,减轻脑损伤、脑积水和铁积累。

IF 3.8 2区 医学 Q1 CLINICAL NEUROLOGY
Tianjie Zhang, Fan Xia, Yingfeng Wan, Guohua Xi, Hua Ya, Richard F Keep
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引用次数: 0

摘要

红细胞溶解释放的血液成分在脑室内出血(IVH)后的继发性脑损伤和出血性脑积水(PHH)中起着重要作用。本研究探讨了补体抑制剂 N-乙酰肝素(NAH)对 IVH 后老龄大鼠早期红细胞溶解、出血性脑积水和铁积累的影响。这项研究以 18 个月大的雄性 Fischer 344 大鼠为对象,分为三个部分。首先,大鼠脑室内注射自体血液(IVH),混合 NAH 或生理盐水,或仅注射生理盐水。4小时后进行核磁共振成像,Western印迹和免疫组化检查补体激活情况,电镜检查脉络丛和脑室周围损伤情况。其次,用 NAH 或载体或生理盐水对大鼠进行 IVH。大鼠在4小时和1天后接受连续核磁共振成像,以评估心室容积和红细胞溶解。免疫组化和 H&E 染色检查继发性脑损伤。第三,用 NAH 或药物对大鼠进行 IVH 治疗。第1天和第28天的连续核磁共振成像可评估脑室容积和铁积累情况。H&E染色和免疫荧光评估了脉络丛吞噬细胞。IVH后4小时发现补体激活,同时注射NAH可抑制补体激活。在IVH后4小时和1天,注射NAH可减轻红细胞溶解,缩小心室容积,减轻脑室周围和脉络丛损伤。在 IVH 后 28 天,NAH 可减少铁的积累、脉络丛吞噬细胞的数量,并减轻脑积水。抑制补体可减少早期红细胞溶解,减轻老年动物IVH后的脑积水和铁积累。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Complement Inhibition Reduces Early Erythrolysis, Attenuates Brain Injury, Hydrocephalus, and Iron Accumulation after Intraventricular Hemorrhage in Aged Rats.

Complement Inhibition Reduces Early Erythrolysis, Attenuates Brain Injury, Hydrocephalus, and Iron Accumulation after Intraventricular Hemorrhage in Aged Rats.

Blood components released by erythrolysis play an important role in secondary brain injury and posthemorrhagic hydrocephalus (PHH) after intraventricular hemorrhage (IVH). The current study examined the impact of N-acetylheparin (NAH), a complement inhibitor, on early erythrolysis, PHH and iron accumulation in aged rats following IVH. This study, on 18-months-old male Fischer 344 rats, was in 3 parts. First, rats had an intracerebroventricular injection of autologous blood (IVH) mixed with NAH or saline, or saline alone. After MRI at four hours, Western blot and immunohistochemistry examined complement activation and electron microscopy choroid plexus and periventricular damage. Second, rats had an IVH with NAH or vehicle, or saline. Rats underwent serial MRI at 4 h and 1 day to assess ventricular volume and erythrolysis. Immunohistochemistry and H&E staining examined secondary brain injury. Third, rats had an IVH with NAH or vehicle. Serial MRIs on day 1 and 28 assessed ventricular volume and iron accumulation. H&E staining and immunofluorescence evaluated choroid plexus phagocytes. Complement activation was found 4 h after IVH, and co-injection of NAH inhibited that activation. NAH administration attenuated erythrolysis, reduced ventricular volume, alleviated periventricular and choroid plexus injury at 4 h and 1 day after IVH. NAH decreased iron accumulation, the number of choroid plexus phagocytes, and attenuated hydrocephalus at 28 days after IVH. Inhibiting complement can reduce early erythrolysis, attenuates hydrocephalus and iron accumulation after IVH in aged animals.

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来源期刊
Translational Stroke Research
Translational Stroke Research CLINICAL NEUROLOGY-NEUROSCIENCES
CiteScore
13.80
自引率
4.30%
发文量
130
审稿时长
6-12 weeks
期刊介绍: Translational Stroke Research covers basic, translational, and clinical studies. The Journal emphasizes novel approaches to help both to understand clinical phenomenon through basic science tools, and to translate basic science discoveries into the development of new strategies for the prevention, assessment, treatment, and enhancement of central nervous system repair after stroke and other forms of neurotrauma. Translational Stroke Research focuses on translational research and is relevant to both basic scientists and physicians, including but not restricted to neuroscientists, vascular biologists, neurologists, neuroimagers, and neurosurgeons.
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