通过生物分子相互作用研究揭示硫化琉球芽孢杆菌质膜电子传递网络。

IF 4.5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Protein Science Pub Date : 2024-07-01 DOI:10.1002/pro.5082
Marisa R Ferreira, Leonor Morgado, Carlos A Salgueiro
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引用次数: 0

摘要

位于不同区室的多血红素细胞色素对硫发生地芽孢杆菌的胞外电子传递至关重要,可推动重要的环境过程和生物技术应用。最近的研究发现,对于特定的电子末端受体,离散的呼吸途径会从内膜和外膜选择性地招募特定的细胞色素。然而,对于丰富的外质细胞色素,即三价细胞色素家族 PpcA-E,却没有观察到这种特异性。在这项工作中,研究人员探索了这些蛋白质在不同氧化还原状态下的独特核磁共振光谱特征,以监测每对细胞色素之间的成对相互作用和电子转移反应。结果表明,这五种蛋白质之间存在瞬时相互作用,并能相互交换电子,这揭示了该家族成员之间的内部互用性。这一发现是在这组细胞色素建立了有效的电子传递网络的背景下进行讨论的。这种网络对细菌十分有利,因为它能使细胞内的功能性工作电位氧化还原范围得以维持。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Periplasmic electron transfer network in Geobacter sulfurreducens revealed by biomolecular interaction studies.

Multiheme cytochromes located in different compartments are crucial for extracellular electron transfer in the bacterium Geobacter sulfurreducens to drive important environmental processes and biotechnological applications. Recent studies have unveiled that for particular sets of electron terminal acceptors, discrete respiratory pathways selectively recruit specific cytochromes from both the inner and outer membranes. However, such specificity was not observed for the abundant periplasmic cytochromes, namely the triheme cytochrome family PpcA-E. In this work, the distinctive NMR spectroscopic signatures of these proteins in different redox states were explored to monitor pairwise interactions and electron transfer reactions between each pair of cytochromes. The results showed that the five proteins interact transiently and can exchange electrons between each other revealing intra-promiscuity within the members of this family. This discovery is discussed in the light of the establishment of an effective electron transfer network by this pool of cytochromes. This network is advantageous to the bacteria as it enables the maintenance of the functional working potential redox range within the cells.

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来源期刊
Protein Science
Protein Science 生物-生化与分子生物学
CiteScore
12.40
自引率
1.20%
发文量
246
审稿时长
1 months
期刊介绍: Protein Science, the flagship journal of The Protein Society, is a publication that focuses on advancing fundamental knowledge in the field of protein molecules. The journal welcomes original reports and review articles that contribute to our understanding of protein function, structure, folding, design, and evolution. Additionally, Protein Science encourages papers that explore the applications of protein science in various areas such as therapeutics, protein-based biomaterials, bionanotechnology, synthetic biology, and bioelectronics. The journal accepts manuscript submissions in any suitable format for review, with the requirement of converting the manuscript to journal-style format only upon acceptance for publication. Protein Science is indexed and abstracted in numerous databases, including the Agricultural & Environmental Science Database (ProQuest), Biological Science Database (ProQuest), CAS: Chemical Abstracts Service (ACS), Embase (Elsevier), Health & Medical Collection (ProQuest), Health Research Premium Collection (ProQuest), Materials Science & Engineering Database (ProQuest), MEDLINE/PubMed (NLM), Natural Science Collection (ProQuest), and SciTech Premium Collection (ProQuest).
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