中国浙江省南部地区野生啮齿动物隐孢子虫的跨物种传播及其对公共卫生的可能影响。

IF 2.3 2区 医学 Q2 PARASITOLOGY
Parasite Pub Date : 2024-01-01 Epub Date: 2024-07-01 DOI:10.1051/parasite/2024033
Yanyan Jiang, Aiying Jiang, Guangxu Ren, Long Wang, Xianming Xin, Zhongying Yuan, Jiani Liu, Zhen Li, Yanbin Sun, Shanshan Zhou, Gang Lu, Huicong Huang, Wei Zhao
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引用次数: 0

摘要

野生啮齿动物是隐孢子虫的贮藏地,在全球范围内数量过多。然而,中国有关这些动物中隐孢子虫的基因数据十分有限。在此,我们测定了从中国浙江省南部三个不同地点捕获的 370 只野生啮齿动物中隐孢子虫的流行率和遗传特征。我们从每只啮齿动物的直肠中采集了新鲜粪便,并从中提取了 DNA。通过 PCR 扩增脊椎动物细胞色素 b 基因,确定了啮齿动物的种类。通过对核糖体 RNA 小亚基基因进行 PCR 扩增和扩增片段测序,检测出隐孢子虫。通过分析 60-kDa 糖蛋白基因,对隐孢子虫和副隐孢子虫的阳性样本进行了进一步分型。7%(26/370)的样本中发现了隐孢子虫阳性结果,涉及 5 种啮齿类动物:它们分别是:Apodemus agrarius (36)、Niviventer niviventer (75)、Rattus losea (18)、R. norvegicus (155) 和 R. tanezumi (86)。它们的隐孢子虫阳性率分别为 8.3%、5.3%、11.1%、7.1% 和 7.0%。序列分析证实了三种隐孢子虫的存在:C.parvum(4 个)、C. viatorum(1 个)和 C. muris(1 个),以及两种基因型:鼠隐孢子虫基因型 IV(16 个)和类鼠隐孢子虫(4 个)。此外,还检测到两种副噬菌体亚型(IIdA15G1 和 IIpA19)和一种隐孢子虫亚型(XVdA3)。这些结果表明,浙江多种野生啮齿类动物同时感染了适应啮齿类动物和人畜共患的隐孢子虫种类/基因型,表明这些啮齿类动物在维持和传播这种寄生虫到环境和其他宿主(包括人类)中起到了一定的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cross-species transmission of Cryptosporidium in wild rodents from the southern region of Zhejiang Province of China and its possible impact on public health.

Wild rodents serve as reservoirs for Cryptosporidium and are overpopulated globally. However, genetic data regarding Cryptosporidium in these animals from China are limited. Here, we have determined the prevalence and genetic characteristics of Cryptosporidium among 370 wild rodents captured from three distinct locations in the southern region of Zhejiang Province, China. Fresh feces were collected from the rectum of each rodent, and DNA was extracted from them. The rodent species was identified by PCR amplifying the vertebrate cytochrome b gene. Cryptosporidium was detected by PCR amplification and amplicon sequencing the small subunit of ribosomal RNA gene. Positive samples of C. viatorum and C. parvum were further subtyped by analyzing the 60-kDa glycoprotein gene. A positive Cryptosporidium result was found in 7% (26/370) of samples, involving five rodent species: Apodemus agrarius (36), Niviventer niviventer (75), Rattus losea (18), R. norvegicus (155), and R. tanezumi (86). Their respective Cryptosporidium positive rates were 8.3%, 5.3%, 11.1%, 7.1%, and 7.0%. Sequence analysis confirmed the presence of three Cryptosporidium species: C. parvum (4), C. viatorum (1), and C. muris (1), and two genotypes: Cryptosporidium rat genotype IV (16) and C. mortiferum-like (4). Additionally, two subtypes of C. parvum (IIdA15G1 and IIpA19) and one subtype of C. viatorum (XVdA3) were detected. These results demonstrate that various wild rodent species in Zhejiang were concurrently infected with rodent-adapted and zoonotic species/genotypes of Cryptosporidium, indicating that these rodents can play a role in maintaining and dispersing this parasite into the environment and other hosts, including humans.

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来源期刊
Parasite
Parasite 医学-寄生虫学
CiteScore
5.50
自引率
6.90%
发文量
49
审稿时长
3 months
期刊介绍: Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.
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