外泌体 miR-196a-5p 通过抑制 ITM2B 促进食管鳞状细胞癌的恶性发展。

IF 2.5 4区 医学 Q2 PATHOLOGY
Pathology International Pub Date : 2024-08-01 Epub Date: 2024-06-28 DOI:10.1111/pin.13459
Min Huang, Shuang Li, Hai Zeng, Yan Zhu, Fan Zhang, Jun Cai
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引用次数: 0

摘要

癌细胞的外泌体可作为载体传播或运输特定的微RNA(miRNA)到远处发挥其作用,但外泌体miRNA在食管鳞状细胞癌(ESCC)中的作用机制尚未得到充分解释。因此,在本研究中,我们对外体miR-196a-5p在ESCC进展中的影响感兴趣。我们发现,miR-196a-5p 在临床组织、ESCC 细胞和外泌体中表达丰富。从功能上讲,耗尽 miR-196a-5p 会阻碍 ESCC 细胞的生长、迁移和侵袭,而过表达 miR-196a-5p 则会产生相反的结果。此外,在受体 ESCC 细胞中增强外泌体 miR-196a-5p 会引发更强烈的增殖和迁移。从机理上讲,我们发现完整膜蛋白2B(ITM2B)是miR-196a-5p的直接靶标。沉默 ITM2B 部分抵消了 miR-196a-5p 抑制剂对 ESCC 恶性表型的抑制作用。此外,在体内,引入抗miR-196a-5p引发的较低miR-196a-5p水平导致产生体积和重量较小的异种移植肿瘤。因此,我们的研究结果证明了外泌体和细胞内miR-196a-5p介导ESCC生长和迁移的新机制,并确定了miR-196a-5p与ITM2B的相互作用。这些工作可能会为开发 ESCC 的临床治疗方案提供新的靶点和依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exosomal miR-196a-5p contributes to esophageal squamous cell carcinoma malignant progression by inhibiting ITM2B.

Exosomes from cancer cells function as carriers to spread or transport specific microRNAs (miRNAs) to distant sites to exert their effects, but the mechanism of exosomal miRNA action in esophageal squamous cell carcinoma (ESCC) has not been fully explained. Therefore, in this study, we were interested in the impact of exosomal miR-196a-5p in ESCC progression. We found that miR-196a-5p was expressed enriched in clinical tissues, ESCC cells, and exosomes. Functionally, depletion of miR-196a-5p impeded ESCC cell growth, migration, and invasion, whereas overexpression of miR-196a-5p produced the opposite results. Moreover, enhancement of exosomal miR-196a-5p in recipient ESCC cells triggered more intense proliferation and migration. Mechanistically, we identified integral membrane protein 2B (ITM2B) as a direct target of miR-196a-5p. Silencing of ITM2B partially counteracted the inhibitory effect of miR-196a-5p inhibitors on the malignant phenotype of ESCC. Furthermore, in vivo, lower miR-196a-5p levels triggered by the introduction of antagomiR-196a-5p resulted in the generation of smaller volume and weight xenograft tumors. Thus, our results demonstrated novel mechanisms of exosomal and intracellular miR-196a-5p-mediated ESCC growth and migration and identify the interaction of miR-196a-5p with ITM2B. These works might provide new targets and basis for the development of clinical treatment options for ESCC.

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来源期刊
Pathology International
Pathology International 医学-病理学
CiteScore
4.50
自引率
4.50%
发文量
102
审稿时长
12 months
期刊介绍: Pathology International is the official English journal of the Japanese Society of Pathology, publishing articles of excellence in human and experimental pathology. The Journal focuses on the morphological study of the disease process and/or mechanisms. For human pathology, morphological investigation receives priority but manuscripts describing the result of any ancillary methods (cellular, chemical, immunological and molecular biological) that complement the morphology are accepted. Manuscript on experimental pathology that approach pathologenesis or mechanisms of disease processes are expected to report on the data obtained from models using cellular, biochemical, molecular biological, animal, immunological or other methods in conjunction with morphology. Manuscripts that report data on laboratory medicine (clinical pathology) without significant morphological contribution are not accepted.
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