评估节肢动物包囊反应的改进方法。

IF 2.3 2区 农林科学 Q1 ENTOMOLOGY
Lucas J. Kirschman, Hannah M. Eastman, Frank J. Irovic, Nathaniel A. Nix, Ly Tuan Kiet Bui, Sydney L. Blackmon, Jaylen S. Greenlee, Rubina Lamichhane, Jack D. Mabuce, Hannah K. McAllister, Lilly F. Nevill, Peyton Redinger, Nia I. Rivers, Jackson L. Sprague
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引用次数: 0

摘要

生态免疫学探索生态因素和进化过程如何影响不同类群的免疫反应,以及免疫反应如何与其他性状进行权衡。研究免疫反应需要有生物意义的免疫测定方法,这些方法应适用于广泛的类群,并具有足够的灵敏度来检测免疫反应的变化。有用的免疫测定还应与免疫能力和适应性相关。包囊反应是节肢动物的一种复杂免疫机制,是生态免疫学研究的有力方法。然而,测试包囊反应的传统方法需要长时间的训练。本研究介绍了一种创新的、具有成本效益的方法,用于评估节肢动物的包囊免疫反应,该方法通过减少所需的培训时间和技能简化了操作过程。我们改进的装置利用笔和注射器组件将单丝插入节肢动物幼虫体内。我们将我们的设备与传统方法进行了比较。尽管新方法快了 22%,但与传统技术相比,它并没有降低封装反应的准确性或有效性,显示出相似的黑色化和封装程度。我们的方法使经验不足的研究人员(如本科生)更容易参与,促进了他们参与生态免疫学研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

An improved method to assess the encapsulation response in arthropods

An improved method to assess the encapsulation response in arthropods

Ecoimmunology explores how ecological factors and evolutionary processes influence immune responses across various taxa and how immune responses trade-off with other traits. Studying immune responses requires biologically meaningful immunoassays applicable to a broad range of taxa and are sensitive enough to detect changes in the immune response. Useful immunoassays should also correlate with immunocompetence and fitness. The encapsulation response, a complex immune mechanism in arthropods, serves as a robust method for ecoimmunological investigations. However, traditional methods to test the encapsulation response can require long training. This study introduces an innovative, cost-effective method for assessing the encapsulation immune response in arthropods, which simplifies the procedure by reducing the training time and skill required. Our modified device utilizes a pen and syringe assembly for inserting monofilaments into arthropod larvae. We compared our device against traditional methods. Despite the new method being 22% faster, it did not compromise the accuracy or effectiveness of the encapsulation response when compared with traditional techniques, demonstrating similar degrees of melanization and encapsulation. Our method allowed for more accessible participation by less experienced researchers, such as undergraduates, facilitating their involvement in ecoimmunological research.

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来源期刊
Journal of insect physiology
Journal of insect physiology 生物-昆虫学
CiteScore
4.50
自引率
4.50%
发文量
77
审稿时长
57 days
期刊介绍: All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.
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