幽门螺杆菌 vacA 等位基因组合、dupA、cagE 和 cagA 基因型及其与摩洛哥人胃病的关系。

IF 1.3 4区 医学 Q4 INFECTIOUS DISEASES
Japanese journal of infectious diseases Pub Date : 2024-11-21 Epub Date: 2024-06-28 DOI:10.7883/yoken.JJID.2024.061
Souad Oirdi Zahir, Mounia El Khadir, Samia Alaoui Boukhris, Dafr-Allah Benajah, Sidi Adil Ibrahimi, Laila Chbani, Mohamed El Abkari, Bahia Bennani
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引用次数: 0

摘要

研究人员对 vacA 的四个区域与 cagA、cagE、dupA 基因和 cagA-EPIYA motifs 的组合进行了研究,以找到最有可能用作摩洛哥人群疾病决定性标记的组合。从征得同意的患者身上共获得了 838 例幽门螺杆菌阳性病例,这些病例之前已通过 PCR 分析确定了 vacA-s -m、-i 区域、cagE 状态和 cagA 3' 区域多态性的特征,现在又用来确定 vacA-d 区域的特征和 dupA 基因的状态。分析结果表明,毒力较弱的组合(vacA(s2m2i2d2)dupA(-)cagE(-)cagA(-))占主导地位,并显示胃癌风险在感染了vacA-d区的患者中高出13.33倍(1.06-166.37)),而感染了携带 vacA(s1m1i1d1)dupA(-)cagE(+)cagA(2EPIYA-C) 的幽门螺杆菌菌株的无病变胃炎患者要比感染了携带 vacA(s2m2i2d2)dupA(-)cagE(-)cagA(-) 的幽门螺杆菌菌株的患者高出 13.33 倍。与无病变的胃炎患者相比,感染携带 vacA(s1m1i1d1)dupA(+)cagE(+)cagA(1EPIYAC) 基因型组合的菌株是胃溃疡和十二指肠溃疡的危险因素(Odds Ratio (95% CI) 分别为 16 (1.09-234.24) 和 6.54 (1.60-26.69))。这些结果表明,vacA 基因型的活性形式、dupA 基因状态和 EPIYA-C 矩阵的数量可被认为是区分多种胃病的有用标记物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Helicobacter pylori vacA Allelic Combination, dupA, cagE and cagA Genotypes and Their Associations with Gastric Diseases in the Moroccan Population.

This study aimed to investigate the combination of the four regions of Helicobacter pylori vacA with cagA, cagE, dupA genes and cagA-EPIYA motifs to identify the most likely combination that could be used as a disease determinant marker in the Moroccan population. A total of 838 H. pylori-positive samples were obtained from consenting patients, that were previously analyzed by PCR to characterize vacA-s, -m, and -i regions; cagE status; and cagA 3' region polymorphism, were used to characterize vacA-d region and to determine dupA gene status. The analysis showed the predominance of the less virulent combination {vacA(s2m2i2d2)dupA(-)cagE(-)cagA(-)}, and showed that the risk of gastric cancer is 13.33 fold higher (95% confidence interval [CI] = 1.06-166.37) in patients infected with strains harboring vacA(s1m1i1d1)dupA(-)cagE(+)cagA(2EPIYA-C) compared to patients with gastritis without lesions and infected by H. pylori strains harboring vacA(s2m2i2d2)dupA(-) cagE(-)cagA(-). Infection with strains harboring the vacA(s1m1i1d1)dupA(+)cagE(+)cagA(1EPIYA-C) genotype combination represented a risk factor for both gastric ulcer and duodenal ulcer than gastritis without lesions; odds ratio (OR) =16 (95% CI = 1.09-234.24) and OR = 12.39 (95% CI = 1.09-140.81), respectively. These results suggest that the combination of the active form of vacA genotypes, dupA gene status, and the number of EPIYA-C motifs may be helpful markers for discriminating between several gastric diseases.

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来源期刊
CiteScore
4.50
自引率
4.50%
发文量
172
审稿时长
2 months
期刊介绍: Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.
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