日本中部地区分离到的产 CTX-M 型广谱 β-内酰胺酶大肠杆菌的分布和抗菌药敏感性模式。

IF 1.3 4区 医学 Q4 INFECTIOUS DISEASES
Japanese journal of infectious diseases Pub Date : 2024-11-21 Epub Date: 2024-06-28 DOI:10.7883/yoken.JJID.2024.079
Kazuya Itadani, Yoshimi Oonishi, Harumi Hisada, Tomoaki Tanaka, Shingo Mizunaga, Yuka Yamagishi, Hiroshige Mikamo
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引用次数: 0

摘要

产广谱β-内酰胺酶(ESBL)大肠埃希菌的广泛流行限制了治疗方案,是一个世界性问题。本研究旨在调查2011年至2017年在日本中部地区分离的204株CTX-M型产ESBLs大肠埃希菌的抗菌药敏感性和ESBL类型。最小抑菌浓度是根据临床和实验室标准研究所的指南确定的。通过 PCR 扩增检测了编码 CTX-M 组 β-内酰胺酶的基因。通过序列分析确定了 CTX-M 亚型。CTX-M-9 是最常检测到的 ESBL 组,CTX-M-27 是最常检测到的 ESBL 基因。产生 CTX-M-15 的菌株对他唑巴坦/哌拉西林(TAZ/PIPC)的敏感率明显低于产生 CTX-M-14 和 -27 的菌株。对次级β-内酰胺酶的其他分析表明,大多数 OXA-1 阳性菌株都是 CTX-M-15 产株(94.7%)。这些菌株对 TAZ/PIPC(47.4%)、舒巴坦/氨苄西林(SBT/ABPC)(0.0%)和阿米卡星(AMK)(73.7%)的敏感率明显低于 OXA-1 阴性菌株,表明产 CTX-M-15 菌株的高非敏感率是由于同时携带 OXA-1 所致。产生 CTX-M-15 的菌株对 TAZ/PIPC、SBT/ABPC 和 AMK 的敏感性降低,这可能是由于共携带 OXA-1 的缘故。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Distribution and Antimicrobial Susceptibility Pattern of CTX-M-type Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolated in Chubu Region, Japan.

The widespread prevalence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli limits treatment options and is a worldwide problem. This study aimed to investigate the antimicrobial susceptibility and ESBL types of 204 strains of CTX-M-type ESBLsproducing E. coli isolated from 2011 to 2017 in the Chubu region of Japan and to identify factors correlated with susceptibility. Minimal inhibitory concentrations were determined in accordance with the guidelines of the Clinical and Laboratory Standards Institute. Genes encoding β-lactamases were detected by PCR amplification. The CTX-M subtypes were determined using sequence analyses. CTX- M-15-producing strains showed significantly lower susceptibility rates to tazobactam/piperacillin (TAZ/ PIPC) than CTX-M-14 and -27-producing strains. Additional analyses of secondary β-lactamases revealed that most of the OXA-1-positive strains were CTX-M-15-producing strains (94.7%). The OXA-1-positive strains displayed significantly lower susceptibility to TAZ/PIPC (47.4%), sulbactam/ ampicillin (0.0%), and amikacin (73.7%) than the OXA-1-negative strains, suggesting that the high non-susceptibility rate of the CTX-M-15-producing strain was due to the co-carriage of OXA-1. Statistical analyses showed that OXA-1-positive strains were present in significant amounts in patients aged ≥65 years, suggesting that older patients have a higher risk of being refractory to treatment.

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来源期刊
CiteScore
4.50
自引率
4.50%
发文量
172
审稿时长
2 months
期刊介绍: Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.
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