Alaa M. Ali , Brooke Chang , Anthony N. Consiglio , Gala Sanchez Van Moer , Matthew J. Powell-Palm , Boris Rubinsky , Simo A. Mäkiharju
{"title":"通过压力测量和光子计数 X 射线计算机断层扫描,对无空腔无冰等时玻璃化进行实验观测。","authors":"Alaa M. Ali , Brooke Chang , Anthony N. Consiglio , Gala Sanchez Van Moer , Matthew J. Powell-Palm , Boris Rubinsky , Simo A. Mäkiharju","doi":"10.1016/j.cryobiol.2024.104935","DOIUrl":null,"url":null,"abstract":"<div><p>Isochoric (constant-volume or volumetrically confined) vitrification has shown potential as an alternative cryopreservation-by-vitrification technique, but the complex processes at play within the chamber are yet poorly characterized, and recent investigations have prompted significant debate around whether a truly isochoric vitrification process (in which the liquid remains completely confined by solid boundaries) is indeed feasible. Based on a recent thermomechanical simulation of a high-concentration Me<sub>2</sub>SO solution, Solanki and Rabin (<em>Cryobiology</em>, <strong>2023</strong>, <em>111</em>, 9–15.) argue that isochoric vitrification is not feasible, because differential thermal contraction of the solution and container will necessarily drive generation of a cavity, corrupting the rigid confinement of the liquid. Here, we provide direct experimental evidence to the contrary, demonstrating cavity-free isochoric vitrification of a ∼3.5 M vitrification solution by combined isochoric pressure measurement (IPM) and photon-counting x-ray computed tomography (PC-CT). We hypothesize that the absence of a cavity is due to the minimal thermal contraction of the solution, which we support with additional volumetric analysis of the PC-CT reconstructions. In total, this study provides experimental evidence both demonstrating the feasibility of isochoric vitrification and highlighting the potential of designing vitrification solutions that exhibit minimal thermal contraction.</p></div>","PeriodicalId":10897,"journal":{"name":"Cryobiology","volume":"116 ","pages":"Article 104935"},"PeriodicalIF":2.3000,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0011224024000907/pdfft?md5=163f75d314f21c10d6b5d26fdd503f4a&pid=1-s2.0-S0011224024000907-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Experimental observation of cavity-free ice-free isochoric vitrification via combined pressure measurements and photon counting x-ray computed tomography\",\"authors\":\"Alaa M. Ali , Brooke Chang , Anthony N. Consiglio , Gala Sanchez Van Moer , Matthew J. Powell-Palm , Boris Rubinsky , Simo A. Mäkiharju\",\"doi\":\"10.1016/j.cryobiol.2024.104935\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Isochoric (constant-volume or volumetrically confined) vitrification has shown potential as an alternative cryopreservation-by-vitrification technique, but the complex processes at play within the chamber are yet poorly characterized, and recent investigations have prompted significant debate around whether a truly isochoric vitrification process (in which the liquid remains completely confined by solid boundaries) is indeed feasible. Based on a recent thermomechanical simulation of a high-concentration Me<sub>2</sub>SO solution, Solanki and Rabin (<em>Cryobiology</em>, <strong>2023</strong>, <em>111</em>, 9–15.) argue that isochoric vitrification is not feasible, because differential thermal contraction of the solution and container will necessarily drive generation of a cavity, corrupting the rigid confinement of the liquid. Here, we provide direct experimental evidence to the contrary, demonstrating cavity-free isochoric vitrification of a ∼3.5 M vitrification solution by combined isochoric pressure measurement (IPM) and photon-counting x-ray computed tomography (PC-CT). We hypothesize that the absence of a cavity is due to the minimal thermal contraction of the solution, which we support with additional volumetric analysis of the PC-CT reconstructions. 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Experimental observation of cavity-free ice-free isochoric vitrification via combined pressure measurements and photon counting x-ray computed tomography
Isochoric (constant-volume or volumetrically confined) vitrification has shown potential as an alternative cryopreservation-by-vitrification technique, but the complex processes at play within the chamber are yet poorly characterized, and recent investigations have prompted significant debate around whether a truly isochoric vitrification process (in which the liquid remains completely confined by solid boundaries) is indeed feasible. Based on a recent thermomechanical simulation of a high-concentration Me2SO solution, Solanki and Rabin (Cryobiology, 2023, 111, 9–15.) argue that isochoric vitrification is not feasible, because differential thermal contraction of the solution and container will necessarily drive generation of a cavity, corrupting the rigid confinement of the liquid. Here, we provide direct experimental evidence to the contrary, demonstrating cavity-free isochoric vitrification of a ∼3.5 M vitrification solution by combined isochoric pressure measurement (IPM) and photon-counting x-ray computed tomography (PC-CT). We hypothesize that the absence of a cavity is due to the minimal thermal contraction of the solution, which we support with additional volumetric analysis of the PC-CT reconstructions. In total, this study provides experimental evidence both demonstrating the feasibility of isochoric vitrification and highlighting the potential of designing vitrification solutions that exhibit minimal thermal contraction.
期刊介绍:
Cryobiology: International Journal of Low Temperature Biology and Medicine publishes research articles on all aspects of low temperature biology and medicine.
Research Areas include:
• Cryoprotective additives and their pharmacological actions
• Cryosurgery
• Freeze-drying
• Freezing
• Frost hardiness in plants
• Hibernation
• Hypothermia
• Medical applications of reduced temperature
• Perfusion of organs
• All pertinent methodologies
Cryobiology is the official journal of the Society for Cryobiology.