马铃薯抗病基因的交替剪接可维持生长和免疫之间的平衡

Biying Sun, Jie Huang, Liang Kong, Chuyun Gao, Fei Zhao, Jiayong Shen, Tian Wang, Kangping Li, Luyao Wang, Yuanchao Wang, Dennis A Halterman, Suomeng Dong
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引用次数: 0

摘要

植物拥有强大而复杂的先天免疫系统来抵御病原体,必须在生长与快速检测和防御病原体之间取得平衡。细胞内具有核苷酸结合富亮氨酸重复(NLR)结构的受体能识别病原体衍生的效应蛋白,从而触发免疫反应。编码 NLR 受体的基因表达受到多方面的精确控制。内含子的替代剪接(AS)对感染的反应经常被观察到,但却鲜为人知。在这里,我们报告了马铃薯(Solanum tuberosum)NLR 基因 RB 的内含子发生了 AS 变化,产生了两种转录异构体,它们协调调节植物免疫和生长平衡。在正常生长过程中,RB 主要以内含子保留异构体 RB_IR 的形式存在,编码一种仅含有 NLR N 端的截短蛋白。晚疫病感染时,病原体会诱导 RB 的内含子剪接,增加 RB_CDS 的丰度,后者编码全长的活性 R 蛋白。通过将 RB 剪接异构体与荧光素酶报告系统融合,我们发现 RB 同源效应物 IPI-O1(又称 Avrblb1)是 RB AS 的促进因子。IPI-O1 与马铃薯剪接因子 StCWC15 直接相互作用,导致 StCWC15 从核质到核仁和核斑点的定位改变。消除 StCWC15 结合的 IPI-O1 基因突变也会破坏 StCWC15 的重新定位和 RB 内含子的剪接。因此,我们的研究揭示了 StCWC15 是一种监视促进因子,它能感知病原体分泌的效应物,并调节 RB 介导的植物免疫与生长之间的权衡,从而拓展了我们对植物与微生物分子相互作用的认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Alternative splicing of a potato disease resistance gene maintains homeostasis between growth and immunity
Plants possess a robust and sophisticated innate immune system against pathogens and must balance growth with rapid pathogen detection and defense. The intracellular receptors with nucleotide-binding leucine-rich repeat (NLR) motifs recognize pathogen-derived effector proteins and thereby trigger the immune response. The expression of genes encoding NLR receptors is precisely controlled in multifaceted ways. The alternative splicing (AS) of introns in response to infection is recurrently observed but poorly understood. Here we report that the potato (Solanum tuberosum) NLR gene RB undergoes AS of its intron, resulting in two transcriptional isoforms, which coordinately regulate plant immunity and growth homeostasis. During normal growth, RB predominantly exists as intron-retained isoform RB_IR, encoding a truncated protein containing only the N-terminus of the NLR. Upon late blight infection, the pathogen induces intron splicing of RB, increasing the abundance of RB_CDS, which encodes a full-length and active R protein. By deploying the RB splicing isoforms fused with a luciferase reporter system, we identified IPI-O1 (also known as Avrblb1), the RB cognate effector, as a facilitator of RB AS. IPI-O1 directly interacts with potato splicing factor StCWC15, resulting in altered localization of StCWC15 from the nucleoplasm to the nucleolus and nuclear speckles. Mutations in IPI-O1 that eliminate StCWC15 binding also disrupt StCWC15 re-localization and RB intron splicing. Thus, our study reveals that StCWC15 serves as a surveillance facilitator that senses the pathogen-secreted effector and regulates the trade-off between RB-mediated plant immunity and growth, expanding our understanding of molecular plant–microbe interactions.
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