Anna Salvian, Daniel Farkas, Marina Ramirez-Moreno, Daniela Torruella-Salas, Antonio Berná, Claudio Avignone-Rossa, John R. Varcoe, Abraham Esteve-Núñez, Siddharth Gadkari
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引用次数: 0
摘要
高效的废水处理监测对于解决水资源短缺问题至关重要。微生物燃料电池(MFC)已成为城市污水生化需氧量(BOD)的实时生物传感器。由于废水成分不同,混合培养的电活性生物膜的组成和新陈代谢可能会发生变化,从而导致信号产生的差异。本研究采用基于三维打印 MFC 的生物传感器来评估无菌复合人工废水和未经处理的城市污水的生化需氧量。使用 16S rRNA 测序和元基因组学分析评估了阳极微生物组成的变化。结果表明,基于 MFC 的生物传感器可针对不同类型的废水进行有效的重新校准,并保持一致的灵敏度(合成废水为 0.64 ± 0.10 mA L mg-1 m-2,城市污水为 0.78 ± 0.13 mA L mg-1 m-2)和检测限(合成废水为 49 ± 8 mg L-1,城市污水为 44 ± 7 mg L-1)。最重要的是,在将废水引入生物传感器之前,无需对废水进行预灭菌、电导率调整和氮气净化。不过,废水中存在的原生好氧微生物可能会影响电流输出。元基因组学和分类学分析表明,生物膜组成的变化主要是对不同基质的不同化学和微生物组成的反应。尽管阳极生物膜的组成发生了变化,但基于 MFC 的生物传感器仍能保持与标准 BOD 测试相当的相对误差。这突出表明,在生物膜完全调整之前,生物传感器可直接用于各种废水类型,具有很强的适应性和灵活性。
Resilience of anodic biofilm in microbial fuel cell biosensor for BOD monitoring of urban wastewater
Efficient wastewater treatment monitoring is vital for addressing water scarcity. Microbial fuel cells (MFCs) have emerged as real-time biosensors for biochemical oxygen demand (BOD) in urban wastewater. Discrepancies in signal generation may arise due to changes in the composition and metabolism of mixed-culture electroactive biofilms stemming from different wastewater compositions. In this study, 3D-printed MFC-based biosensors were employed to assess the BOD of sterile complex artificial wastewater and untreated urban wastewater. Alterations in the microbial composition of the anode were evaluated using 16S rRNA sequencing and metagenomics analysis. Results show that MFC-based biosensors can be effectively recalibrated for diverse types of wastewater, maintaining consistent sensitivity (0.64 ± 0.10 mA L mg−1 m−2 with synthetic wastewater and 0.78 ± 0.13 mA L mg−1 m−2 with urban wastewater) and limit of detection (49 ± 8 mg L−1 for synthetic wastewater and 44 ± 7 mg L−1 for urban wastewater). Crucially, pre-sterilization, conductivity adjustments, and nitrogen purging of wastewater are not required before its introduction into the biosensor. However, the presence of native aerobic microorganisms in the wastewater might affect the current output. Metagenomics and taxonomic analyses revealed that the alterations in biofilm composition are predominantly in response to the varied chemical and microbiological compositions of different substrates. Despite variations in anodic biofilm composition, the MFC-based biosensor maintains a relative error comparable to the standard BOD test. This highlights the resilience and flexibility of the biosensor when directly used with a variety of wastewater types before full biofilm adjustment.
npj Clean WaterEnvironmental Science-Water Science and Technology
CiteScore
15.30
自引率
2.60%
发文量
61
审稿时长
5 weeks
期刊介绍:
npj Clean Water publishes high-quality papers that report cutting-edge science, technology, applications, policies, and societal issues contributing to a more sustainable supply of clean water. The journal's publications may also support and accelerate the achievement of Sustainable Development Goal 6, which focuses on clean water and sanitation.