核酸适配体能抵御铅(Pb(II)毒性。

IF 4.5 2区 生物学 Q1 BIOCHEMICAL RESEARCH METHODS
Afreen Anwar , Solimar Ramis De Ayreflor Reyes , Aijaz Ahmad John , Erik Breiling , Abigail M. O’Connor , Stephanie Reis , Jae-Hyuck Shim , Ali Asghar Shah , Jagan Srinivasan , Natalie G. Farny
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引用次数: 0

摘要

铅(Pb(II))是一种普遍存在的重金属毒素,对人类健康有许多公认的负面影响。铅的毒性源于累积、反复的环境暴露。因此,防止铅生物蓄积的预防性策略可以减少与铅相关的人类病症。在这里,我们展示了 DNA 和 RNA aptamers 可保护秀丽隐杆线虫免受铅引起的毒性表型的影响。通过育雏规模测定,与DNA或RNA适配体共同喂养动物可防止生殖毒性。同样,通过行为测定法测量的铅诱导的神经毒性也会在喂食适配体后恢复正常。此外,培养的人类 HEK293 和原代小鼠成骨细胞通过转染 DNA 短链对铅毒性也有保护作用。事先转染与铅结合的DNA适配体后,因铅暴露而减少的成骨发育得以维持。面对日益增多的环境毒物,适配体可能是保护人类健康的有效策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nucleic acid aptamers protect against lead (Pb(II)) toxicity

Lead (Pb(II)) is a pervasive heavy metal toxin with many well-established negative effects on human health. Lead toxicity arises from cumulative, repeated environmental exposures. Thus, prophylactic strategies to protect against the bioaccumulation of lead could reduce lead-associated human pathologies. Here we show that DNA and RNA aptamers protect C. elegans from toxic phenotypes caused by lead. Reproductive toxicity, as measured by brood size assays, is prevented by co-feeding of animals with DNA or RNA aptamers. Similarly, lead-induced neurotoxicity, measured by behavioral assays, are also normalized by aptamer feeding. Further, cultured human HEK293 and primary murine osteoblasts are protected from lead toxicity by transfection with DNA aptamers. The osteogenic development, which is decreased by lead exposure, is maintained by prior transfection of lead-binding DNA aptamers. Aptamers may be an effective strategy for the protection of human health in the face of increasing environmental toxicants.

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来源期刊
New biotechnology
New biotechnology 生物-生化研究方法
CiteScore
11.40
自引率
1.90%
发文量
77
审稿时长
1 months
期刊介绍: New Biotechnology is the official journal of the European Federation of Biotechnology (EFB) and is published bimonthly. It covers both the science of biotechnology and its surrounding political, business and financial milieu. The journal publishes peer-reviewed basic research papers, authoritative reviews, feature articles and opinions in all areas of biotechnology. It reflects the full diversity of current biotechnology science, particularly those advances in research and practice that open opportunities for exploitation of knowledge, commercially or otherwise, together with news, discussion and comment on broader issues of general interest and concern. The outlook is fully international. The scope of the journal includes the research, industrial and commercial aspects of biotechnology, in areas such as: Healthcare and Pharmaceuticals; Food and Agriculture; Biofuels; Genetic Engineering and Molecular Biology; Genomics and Synthetic Biology; Nanotechnology; Environment and Biodiversity; Biocatalysis; Bioremediation; Process engineering.
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