一种来自霉菌的新型木聚糖酶会引发烟草中的植物免疫反应。

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES
Yuqiang Zhao, Kun Yang, Yanxin Wang, Xu Li, Chengyao Xia, Yan Huang, Zhoukun Li, Cancan Zhu, Zhongli Cui, Xianfeng Ye
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引用次数: 0

摘要

众所周知,从真菌(包括植物病原真菌和非病原真菌)中提取的木聚糖酶可引发植物免疫反应。然而,有关细菌衍生的木聚糖酶触发植物免疫反应能力的研究却很有限。本文从囊胞杆菌(Cystobacter sp. 0969)中发现了一种名为 CcXyn 的新型木聚糖酶,它对植物病原真菌和细菌都具有广谱活性。CcXyn 属于糖苷水解酶(GH)11 家族,与已知会引发植物免疫反应的真菌木聚糖酶的序列相同度约为 32.0%-45.0%。用纯化的 CcXyn 处理烟草,可诱导超敏反应(HR)和防御反应,如产生活性氧(ROS)和上调防御基因的表达,最终增强烟草对烟草疫霉的抗性。这些研究结果表明,CcXyn 是一种微生物相关分子模式(MAMP)诱导剂,可引起植物免疫反应,与其酶活性无关。与真菌木聚糖酶类似,CcXyn 也能被 N. benthamiana 细胞膜上的 NbRXEGL1 受体识别。下游信号传导与 BAK1 和 SOBIR1 共受体无关,这表明在 N. benthamiana 中 CcXyn 被识别后,其他共受体参与了信号传导。此外,其他木霉菌中的木聚糖酶也有能力触发本根玉米中的植物免疫反应,这表明木霉菌中的木聚糖酶在触发植物免疫功能方面无所不在。这项研究拓展了人们对具有植物免疫反应诱导特性的木聚糖酶的认识,并为粘杆菌在针对植物病原体的生物防治策略中的潜在应用提供了理论基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A novel xylanase from a myxobacterium triggers a plant immune response in Nicotiana benthamiana.

Xylanases derived from fungi, including phytopathogenic and nonpathogenic fungi, are commonly known to trigger plant immune responses. However, there is limited research on the ability of bacterial-derived xylanases to trigger plant immunity. Here, a novel xylanase named CcXyn was identified from the myxobacterium Cystobacter sp. 0969, which displays broad-spectrum activity against both phytopathogenic fungi and bacteria. CcXyn belongs to the glycoside hydrolases (GH) 11 family and shares a sequence identity of approximately 32.0%-45.0% with fungal xylanases known to trigger plant immune responses. Treatment of Nicotiana benthamiana with purified CcXyn resulted in the induction of hypersensitive response (HR) and defence responses, such as the production of reactive oxygen species (ROS) and upregulation of defence gene expression, ultimately enhancing the resistance of N. benthamiana to Phytophthora nicotianae. These findings indicated that CcXyn functions as a microbe-associated molecular pattern (MAMP) elicitor for plant immune responses, independent of its enzymatic activity. Similar to fungal xylanases, CcXyn was recognized by the NbRXEGL1 receptor on the cell membrane of N. benthamiana. Downstream signalling was shown to be independent of the BAK1 and SOBIR1 co-receptors, indicating the involvement of other co-receptors in signal transduction following CcXyn recognition in N. benthamiana. Moreover, xylanases from other myxobacteria also demonstrated the capacity to trigger plant immune responses in N. benthamiana, indicating that xylanases in myxobacteria are ubiquitous in triggering plant immune functions. This study expands the understanding of xylanases with plant immune response-inducing properties and provides a theoretical basis for potential applications of myxobacteria in biocontrol strategies against phytopathogens.

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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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