人类接种流感病毒疫苗后生殖中心 B 细胞的成熟。

IF 12.6 1区 医学 Q1 IMMUNOLOGY
Journal of Experimental Medicine Pub Date : 2024-08-05 Epub Date: 2024-06-27 DOI:10.1084/jem.20240668
Katherine M McIntire, Hailong Meng, Ting-Hui Lin, Wooseob Kim, Nina E Moore, Julianna Han, Meagan McMahon, Meng Wang, Sameer Kumar Malladi, Bassem M Mohammed, Julian Q Zhou, Aaron J Schmitz, Kenneth B Hoehn, Juan Manuel Carreño, Temima Yellin, Teresa Suessen, William D Middleton, Sharlene A Teefey, Rachel M Presti, Florian Krammer, Jackson S Turner, Andrew B Ward, Ian A Wilson, Steven H Kleinstein, Ali H Ellebedy
{"title":"人类接种流感病毒疫苗后生殖中心 B 细胞的成熟。","authors":"Katherine M McIntire, Hailong Meng, Ting-Hui Lin, Wooseob Kim, Nina E Moore, Julianna Han, Meagan McMahon, Meng Wang, Sameer Kumar Malladi, Bassem M Mohammed, Julian Q Zhou, Aaron J Schmitz, Kenneth B Hoehn, Juan Manuel Carreño, Temima Yellin, Teresa Suessen, William D Middleton, Sharlene A Teefey, Rachel M Presti, Florian Krammer, Jackson S Turner, Andrew B Ward, Ian A Wilson, Steven H Kleinstein, Ali H Ellebedy","doi":"10.1084/jem.20240668","DOIUrl":null,"url":null,"abstract":"<p><p>Germinal centers (GC) are microanatomical lymphoid structures where affinity-matured memory B cells and long-lived bone marrow plasma cells are primarily generated. It is unclear how the maturation of B cells within the GC impacts the breadth and durability of B cell responses to influenza vaccination in humans. We used fine needle aspiration of draining lymph nodes to longitudinally track antigen-specific GC B cell responses to seasonal influenza vaccination. Antigen-specific GC B cells persisted for at least 13 wk after vaccination in two out of seven individuals. Monoclonal antibodies (mAbs) derived from persisting GC B cell clones exhibit enhanced binding affinity and breadth to influenza hemagglutinin (HA) antigens compared with related GC clonotypes isolated earlier in the response. Structural studies of early and late GC-derived mAbs from one clonal lineage in complex with H1 and H5 HAs revealed an altered binding footprint. Our study shows that inducing sustained GC reactions after influenza vaccination in humans supports the maturation of responding B cells.</p>","PeriodicalId":15760,"journal":{"name":"Journal of Experimental Medicine","volume":"221 8","pages":""},"PeriodicalIF":12.6000,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11211068/pdf/","citationCount":"0","resultStr":"{\"title\":\"Maturation of germinal center B cells after influenza virus vaccination in humans.\",\"authors\":\"Katherine M McIntire, Hailong Meng, Ting-Hui Lin, Wooseob Kim, Nina E Moore, Julianna Han, Meagan McMahon, Meng Wang, Sameer Kumar Malladi, Bassem M Mohammed, Julian Q Zhou, Aaron J Schmitz, Kenneth B Hoehn, Juan Manuel Carreño, Temima Yellin, Teresa Suessen, William D Middleton, Sharlene A Teefey, Rachel M Presti, Florian Krammer, Jackson S Turner, Andrew B Ward, Ian A Wilson, Steven H Kleinstein, Ali H Ellebedy\",\"doi\":\"10.1084/jem.20240668\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Germinal centers (GC) are microanatomical lymphoid structures where affinity-matured memory B cells and long-lived bone marrow plasma cells are primarily generated. It is unclear how the maturation of B cells within the GC impacts the breadth and durability of B cell responses to influenza vaccination in humans. We used fine needle aspiration of draining lymph nodes to longitudinally track antigen-specific GC B cell responses to seasonal influenza vaccination. Antigen-specific GC B cells persisted for at least 13 wk after vaccination in two out of seven individuals. Monoclonal antibodies (mAbs) derived from persisting GC B cell clones exhibit enhanced binding affinity and breadth to influenza hemagglutinin (HA) antigens compared with related GC clonotypes isolated earlier in the response. Structural studies of early and late GC-derived mAbs from one clonal lineage in complex with H1 and H5 HAs revealed an altered binding footprint. Our study shows that inducing sustained GC reactions after influenza vaccination in humans supports the maturation of responding B cells.</p>\",\"PeriodicalId\":15760,\"journal\":{\"name\":\"Journal of Experimental Medicine\",\"volume\":\"221 8\",\"pages\":\"\"},\"PeriodicalIF\":12.6000,\"publicationDate\":\"2024-08-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11211068/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Experimental Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1084/jem.20240668\",\"RegionNum\":1,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/6/27 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Experimental Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1084/jem.20240668","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/6/27 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

生殖中心(GC)是一种微解剖淋巴结构,亲和成熟的记忆性 B 细胞和长寿命骨髓浆细胞主要在此产生。目前还不清楚B细胞在生殖中心内的成熟如何影响人类B细胞对流感疫苗反应的广度和持久性。我们采用细针抽吸引流淋巴结的方法,纵向追踪抗原特异性 GC B 细胞对季节性流感疫苗接种的反应。七个人中有两个人的抗原特异性GC B细胞在接种疫苗后至少持续了13周。从持续存在的GC B细胞克隆中提取的单克隆抗体(mAbs)与反应早期分离出的相关GC克隆相比,对流感血凝素(HA)抗原的结合亲和力更强,结合广度更大。对来自一个克隆系的早期和晚期 GC 衍生 mAbs 与 H1 和 H5 HAs 复合物的结构研究显示,结合足迹发生了改变。我们的研究表明,在人类接种流感疫苗后诱导持续的 GC 反应有助于应答 B 细胞的成熟。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Maturation of germinal center B cells after influenza virus vaccination in humans.

Germinal centers (GC) are microanatomical lymphoid structures where affinity-matured memory B cells and long-lived bone marrow plasma cells are primarily generated. It is unclear how the maturation of B cells within the GC impacts the breadth and durability of B cell responses to influenza vaccination in humans. We used fine needle aspiration of draining lymph nodes to longitudinally track antigen-specific GC B cell responses to seasonal influenza vaccination. Antigen-specific GC B cells persisted for at least 13 wk after vaccination in two out of seven individuals. Monoclonal antibodies (mAbs) derived from persisting GC B cell clones exhibit enhanced binding affinity and breadth to influenza hemagglutinin (HA) antigens compared with related GC clonotypes isolated earlier in the response. Structural studies of early and late GC-derived mAbs from one clonal lineage in complex with H1 and H5 HAs revealed an altered binding footprint. Our study shows that inducing sustained GC reactions after influenza vaccination in humans supports the maturation of responding B cells.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
26.60
自引率
1.30%
发文量
189
审稿时长
3-8 weeks
期刊介绍: Since its establishment in 1896, the Journal of Experimental Medicine (JEM) has steadfastly pursued the publication of enduring and exceptional studies in medical biology. In an era where numerous publishing groups are introducing specialized journals, we recognize the importance of offering a distinguished platform for studies that seamlessly integrate various disciplines within the pathogenesis field. Our unique editorial system, driven by a commitment to exceptional author service, involves two collaborative groups of editors: professional editors with robust scientific backgrounds and full-time practicing scientists. Each paper undergoes evaluation by at least one editor from both groups before external review. Weekly editorial meetings facilitate comprehensive discussions on papers, incorporating external referee comments, and ensure swift decisions without unnecessary demands for extensive revisions. Encompassing human studies and diverse in vivo experimental models of human disease, our focus within medical biology spans genetics, inflammation, immunity, infectious disease, cancer, vascular biology, metabolic disorders, neuroscience, and stem cell biology. We eagerly welcome reports ranging from atomic-level analyses to clinical interventions that unveil new mechanistic insights.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信