用于组织再生的 P(3HB-co-4HB)-生物活性玻璃-石墨烯复合生物材料的生物学和治疗学评估。

IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL
Nik S. A. N. Sharifulden, Lady V. Barrios Silva, Nandin-Erdene Mandakhbayar, Seong-Jin Shin, Hae-Won Kim, Jonathan C. Knowles, Linh T. B. Nguyen, David Y. S. Chau
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引用次数: 0

摘要

理想的伤口敷料应能创造一个愈合环境,缓解疼痛、防止感染、保持湿度、清除碎屑并加速伤口闭合和修复。然而,纱布等传统敷料往往无法满足这些要求,尤其是对于慢性或不愈合的伤口。因此,亟需能提供高效、经济、环保替代品的创新配方。本研究重点评估了基于微生物衍生共聚物聚(3-羟基丁酸-4-羟基丁酸)、P(3HB-co-4HB)生物活性玻璃和石墨烯颗粒的创新配方,并探索了它们在体外和体内的生物反应,以找到促进细胞粘附和增强伤口愈合的最佳组合。在生物活性玻璃(1 w/w%)和石墨烯(0.01 w/w%)浓度下优化的配方显示出加速降解和增强血管形成的效果。同时,使用小鼠成骨细胞、人类真皮成纤维细胞和标准细胞培养实验对生物相容性进行了评估,结果表明培养 7 天后无不良影响,炎症动力学调节良好。使用小鼠进行的全厚度皮肤缺损试验表明,生物复合材料可加快伤口愈合并减少炎症反应。总之,这种生物复合材料有望成为一种理想的伤口敷料,并对伤口愈合率产生积极影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The biological and therapeutic assessment of a P(3HB-co-4HB)-bioactive glass-graphene composite biomaterial for tissue regeneration

The biological and therapeutic assessment of a P(3HB-co-4HB)-bioactive glass-graphene composite biomaterial for tissue regeneration

An ideal wound dressing should create a healing environment that relieves pain, protects against infections, maintains moisture, removes debris, and speeds up wound closure and repair. However, conventional options like gauze often fall short in fulfilling these requirements, especially for chronic or nonhealing wounds. Hence there is a critical need for inventive formulations that offer efficient, cost-effective, and eco-friendly alternatives. This study focuses on assessing the innovative formulation based on a microbial-derived copolymer known as poly(3-hydroxybutyrate-co-4-hydroxybutyrate), P(3HB-co-4HB) bioactive glass and graphene particles, and exploring their biological response in vitro and in vivo—to find the best combination that promotes cell adhesion and enhances wound healing. The formulation optimized at concentration of bioactive glass (1 w/w%) and graphene (0.01 w/w%) showed accelerated degradation and enhanced blood vessel formation. Meanwhile biocompatibility was evaluated using murine osteoblasts, human dermal fibroblasts, and standard cell culture assays, demonstrating no adverse effects after 7 days of culture and well-regulated inflammatory kinetics. Whole thickness skin defect using mice indicated the feasibility of the biocomposites for a faster wound closure and reduced inflammation. Overall, this biocomposite appears promising as an ideal wound dressing material and positively influencing wound healing rates.

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来源期刊
CiteScore
7.50
自引率
2.90%
发文量
199
审稿时长
12 months
期刊介绍: Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.
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