Helder Fernandes de Oliveira, Luan Carlos Gomes Teixeira, Ismael Fernandes Da Silva Júnior, Francisco Ferreira Da Cruz, Hellen Cristina Silva, Alex Da Rocha Gonçalves, Daniel De Almeida Decurcio, Orlando Aguirre Guedes
{"title":"消毒方案对减少下颌臼齿细菌数量的影响","authors":"Helder Fernandes de Oliveira, Luan Carlos Gomes Teixeira, Ismael Fernandes Da Silva Júnior, Francisco Ferreira Da Cruz, Hellen Cristina Silva, Alex Da Rocha Gonçalves, Daniel De Almeida Decurcio, Orlando Aguirre Guedes","doi":"10.14744/hnhj.2024.47450","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Evaluate the bacterial reduction effect of disinfection protocols used in lower molars infected by Enterococcus faecalis.</p><p><strong>Methods: </strong>Eighty extracted lower molars were prepared and inoculated with E. faecalis for 2 months. The teeth were then distributed into 2 control groups (n = 4) and 4 experimental groups (n = 18) in function of the disinfection protocol utilized: G1. WaveOne Gold (WOG), passive ultrasonic irrigation (PUI) and Ultracal; G2. WOG, PUI and calcium hydroxide (CH); G3. WOG, XP-Endo Finisher (XPF) and Ultracal; G4. WOG, XPF and CH. Bacteriological samples were collected previously (S1), after preparation (S2), final agitation (S3) and intracanal dressing (S4). Microbial growth was assessed according to culture turbidity and UV spectrophotometry. Statistical analyses used the Friedman test for paired samples and Kruskal-Wallis test for non-paired data (p<0.05).</p><p><strong>Results: </strong>No protocol eliminated E. faecalis effectively. The S2, S3 and S4 samples were statistically different from the S1 samples in G1, G2, G3 and G4 (p<0.05). Statistical differences were observed in bacterial reduction between G1 and G2 and G1 and G3 after the intracanal medicament (S4) (p<0.05).</p><p><strong>Conclusion: </strong>The WOG + PUI + CH disinfection protocol showed higher percentages of bacterial reduction.</p>","PeriodicalId":11860,"journal":{"name":"European Endodontic Journal","volume":" ","pages":"236-242"},"PeriodicalIF":1.6000,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11413596/pdf/","citationCount":"0","resultStr":"{\"title\":\"Effect of Disinfection Protocols on Bacterial Reduction in Mandibular Molars.\",\"authors\":\"Helder Fernandes de Oliveira, Luan Carlos Gomes Teixeira, Ismael Fernandes Da Silva Júnior, Francisco Ferreira Da Cruz, Hellen Cristina Silva, Alex Da Rocha Gonçalves, Daniel De Almeida Decurcio, Orlando Aguirre Guedes\",\"doi\":\"10.14744/hnhj.2024.47450\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Evaluate the bacterial reduction effect of disinfection protocols used in lower molars infected by Enterococcus faecalis.</p><p><strong>Methods: </strong>Eighty extracted lower molars were prepared and inoculated with E. faecalis for 2 months. The teeth were then distributed into 2 control groups (n = 4) and 4 experimental groups (n = 18) in function of the disinfection protocol utilized: G1. WaveOne Gold (WOG), passive ultrasonic irrigation (PUI) and Ultracal; G2. WOG, PUI and calcium hydroxide (CH); G3. WOG, XP-Endo Finisher (XPF) and Ultracal; G4. WOG, XPF and CH. Bacteriological samples were collected previously (S1), after preparation (S2), final agitation (S3) and intracanal dressing (S4). Microbial growth was assessed according to culture turbidity and UV spectrophotometry. Statistical analyses used the Friedman test for paired samples and Kruskal-Wallis test for non-paired data (p<0.05).</p><p><strong>Results: </strong>No protocol eliminated E. faecalis effectively. The S2, S3 and S4 samples were statistically different from the S1 samples in G1, G2, G3 and G4 (p<0.05). Statistical differences were observed in bacterial reduction between G1 and G2 and G1 and G3 after the intracanal medicament (S4) (p<0.05).</p><p><strong>Conclusion: </strong>The WOG + PUI + CH disinfection protocol showed higher percentages of bacterial reduction.</p>\",\"PeriodicalId\":11860,\"journal\":{\"name\":\"European Endodontic Journal\",\"volume\":\" \",\"pages\":\"236-242\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-08-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11413596/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"European Endodontic Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.14744/hnhj.2024.47450\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Endodontic Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14744/hnhj.2024.47450","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Effect of Disinfection Protocols on Bacterial Reduction in Mandibular Molars.
Objective: Evaluate the bacterial reduction effect of disinfection protocols used in lower molars infected by Enterococcus faecalis.
Methods: Eighty extracted lower molars were prepared and inoculated with E. faecalis for 2 months. The teeth were then distributed into 2 control groups (n = 4) and 4 experimental groups (n = 18) in function of the disinfection protocol utilized: G1. WaveOne Gold (WOG), passive ultrasonic irrigation (PUI) and Ultracal; G2. WOG, PUI and calcium hydroxide (CH); G3. WOG, XP-Endo Finisher (XPF) and Ultracal; G4. WOG, XPF and CH. Bacteriological samples were collected previously (S1), after preparation (S2), final agitation (S3) and intracanal dressing (S4). Microbial growth was assessed according to culture turbidity and UV spectrophotometry. Statistical analyses used the Friedman test for paired samples and Kruskal-Wallis test for non-paired data (p<0.05).
Results: No protocol eliminated E. faecalis effectively. The S2, S3 and S4 samples were statistically different from the S1 samples in G1, G2, G3 and G4 (p<0.05). Statistical differences were observed in bacterial reduction between G1 and G2 and G1 and G3 after the intracanal medicament (S4) (p<0.05).
Conclusion: The WOG + PUI + CH disinfection protocol showed higher percentages of bacterial reduction.
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