{"title":"Faecalibacterium wellingii sp.","authors":"","doi":"10.1016/j.anaerobe.2024.102881","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p>The genus <em>Faecalibacterium</em> is one of the most important butyrate producers in the human intestinal tract and has been widely linked to health. Recently, several different species have been described, but still more phylogroups have been identified, suggesting that additional species may exist. Four strains HTF-F<sup>T</sup>, HTF-128, HTF-75H and HTF-76H, representing two different phylogenetic clusters, are evaluated in this study.</p></div><div><h3>Methods</h3><p>Phylogenomic analysis was performed using whole-genome sequences and 16S rRNA gene sequences. Chemotaxonomic analysis was done based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Physiological and phenotypical characteristics of these strains were also determined. All characteristics of these strains were compared with other validly published species within the genus <em>Faecalibacterium</em>.</p></div><div><h3>Results</h3><p>On a genomic level, the strains HTF-F<sup>T</sup> and HTF-128 shared an average nucleotide identity (ANI) of <95.0 % and digital DNA-DNA hybridization (dDDH) of <70.0 with other <em>Faecalibacterium</em> species, while between HTF-F<sup>T</sup> and HTF-128 the ANI-value was 97.18 % and the dDDH was 76.8 %. HTF-75H and HTF-76H had an ANI and dDDH value of 100 % (99.96 %) and 100 % (99.99 %) respectively. Both HTF-75H and HTF-76H were closely related to <em>Faecalibacterium taiwanense</em> HLW78<sup>T</sup>. 16S rRNA gene and chemotaxonomic analysis were in accordance with the genomic data, confirming that HTF-F<sup>T</sup> and HTF-128 represent a novel <em>Faecalibacterium</em> species and HTF-75H and HTF-76H belong to <em>F. taiwanense</em>.</p></div><div><h3>Conclusions</h3><p><em>Faecalibacterium</em> strains HTF-F<sup>T</sup> (=DSM 117771<sup>T</sup> = NCIMB 15531<sup>T</sup>) and HTF-128 represent a novel species. The name <em>Faecalibacterium wellingii</em> with HTF-F<sup>T</sup> as type strain is proposed. Two novel isolates HTF-75H (=DSM 17770 = NCIMB 15530) and HTF-76H are described in this study and belong to the recently described <em>Faecalibacterium taiwanense.</em></p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"89 ","pages":"Article 102881"},"PeriodicalIF":2.5000,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Description of Faecalibacterium wellingii sp. nov. and two Faecalibacterium taiwanense strains, aiding to the reclassification of Faecalibacterium species\",\"authors\":\"\",\"doi\":\"10.1016/j.anaerobe.2024.102881\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><p>The genus <em>Faecalibacterium</em> is one of the most important butyrate producers in the human intestinal tract and has been widely linked to health. Recently, several different species have been described, but still more phylogroups have been identified, suggesting that additional species may exist. Four strains HTF-F<sup>T</sup>, HTF-128, HTF-75H and HTF-76H, representing two different phylogenetic clusters, are evaluated in this study.</p></div><div><h3>Methods</h3><p>Phylogenomic analysis was performed using whole-genome sequences and 16S rRNA gene sequences. Chemotaxonomic analysis was done based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Physiological and phenotypical characteristics of these strains were also determined. All characteristics of these strains were compared with other validly published species within the genus <em>Faecalibacterium</em>.</p></div><div><h3>Results</h3><p>On a genomic level, the strains HTF-F<sup>T</sup> and HTF-128 shared an average nucleotide identity (ANI) of <95.0 % and digital DNA-DNA hybridization (dDDH) of <70.0 with other <em>Faecalibacterium</em> species, while between HTF-F<sup>T</sup> and HTF-128 the ANI-value was 97.18 % and the dDDH was 76.8 %. HTF-75H and HTF-76H had an ANI and dDDH value of 100 % (99.96 %) and 100 % (99.99 %) respectively. Both HTF-75H and HTF-76H were closely related to <em>Faecalibacterium taiwanense</em> HLW78<sup>T</sup>. 16S rRNA gene and chemotaxonomic analysis were in accordance with the genomic data, confirming that HTF-F<sup>T</sup> and HTF-128 represent a novel <em>Faecalibacterium</em> species and HTF-75H and HTF-76H belong to <em>F. taiwanense</em>.</p></div><div><h3>Conclusions</h3><p><em>Faecalibacterium</em> strains HTF-F<sup>T</sup> (=DSM 117771<sup>T</sup> = NCIMB 15531<sup>T</sup>) and HTF-128 represent a novel species. The name <em>Faecalibacterium wellingii</em> with HTF-F<sup>T</sup> as type strain is proposed. Two novel isolates HTF-75H (=DSM 17770 = NCIMB 15530) and HTF-76H are described in this study and belong to the recently described <em>Faecalibacterium taiwanense.</em></p></div>\",\"PeriodicalId\":8050,\"journal\":{\"name\":\"Anaerobe\",\"volume\":\"89 \",\"pages\":\"Article 102881\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-06-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Anaerobe\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1075996424000647\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anaerobe","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1075996424000647","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Description of Faecalibacterium wellingii sp. nov. and two Faecalibacterium taiwanense strains, aiding to the reclassification of Faecalibacterium species
Objectives
The genus Faecalibacterium is one of the most important butyrate producers in the human intestinal tract and has been widely linked to health. Recently, several different species have been described, but still more phylogroups have been identified, suggesting that additional species may exist. Four strains HTF-FT, HTF-128, HTF-75H and HTF-76H, representing two different phylogenetic clusters, are evaluated in this study.
Methods
Phylogenomic analysis was performed using whole-genome sequences and 16S rRNA gene sequences. Chemotaxonomic analysis was done based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Physiological and phenotypical characteristics of these strains were also determined. All characteristics of these strains were compared with other validly published species within the genus Faecalibacterium.
Results
On a genomic level, the strains HTF-FT and HTF-128 shared an average nucleotide identity (ANI) of <95.0 % and digital DNA-DNA hybridization (dDDH) of <70.0 with other Faecalibacterium species, while between HTF-FT and HTF-128 the ANI-value was 97.18 % and the dDDH was 76.8 %. HTF-75H and HTF-76H had an ANI and dDDH value of 100 % (99.96 %) and 100 % (99.99 %) respectively. Both HTF-75H and HTF-76H were closely related to Faecalibacterium taiwanense HLW78T. 16S rRNA gene and chemotaxonomic analysis were in accordance with the genomic data, confirming that HTF-FT and HTF-128 represent a novel Faecalibacterium species and HTF-75H and HTF-76H belong to F. taiwanense.
Conclusions
Faecalibacterium strains HTF-FT (=DSM 117771T = NCIMB 15531T) and HTF-128 represent a novel species. The name Faecalibacterium wellingii with HTF-FT as type strain is proposed. Two novel isolates HTF-75H (=DSM 17770 = NCIMB 15530) and HTF-76H are described in this study and belong to the recently described Faecalibacterium taiwanense.
期刊介绍：
Anaerobe is essential reading for those who wish to remain at the forefront of discoveries relating to life processes of strictly anaerobes. The journal is multi-disciplinary, and provides a unique forum for those investigating anaerobic organisms that cause infections in humans and animals, as well as anaerobes that play roles in microbiomes or environmental processes.
Anaerobe publishes reviews, mini reviews, original research articles, notes and case reports. Relevant topics fall into the broad categories of anaerobes in human and animal diseases, anaerobes in the microbiome, anaerobes in the environment, diagnosis of anaerobes in clinical microbiology laboratories, molecular biology, genetics, pathogenesis, toxins and antibiotic susceptibility of anaerobic bacteria.
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