开发用于水稻 G 到 T 编辑的鸟嘌呤碱基编辑器。

IF 9.3 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Integrative Plant Biology Pub Date : 2024-06-27 DOI:10.1111/jipb.13729

Lang Liu, Zhongming Zhang, Chenyang Wang, Fang Yan, Wenxian Sun, Xueping Zhou, Weiguo Miao, Huanbin Zhou

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引用次数: 0

摘要

利用工程化的 N-甲基嘌呤 DNA 糖基化酶和 CRISPR 系统创建的两个鸟嘌呤碱基编辑器在水稻（Oryza sativa）中实现了靶向 G 到 T 编辑，效率为 4.94-12.50%。DNA 糖基化酶和脱氨酶的联合使用实现了目标鸟嘌呤与腺嘌呤或胞嘧啶的共同编辑。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Developing guanine base editors for G-to-T editing in rice

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Developing guanine base editors for G-to-T editing in rice

Two guanine base editors created using an engineered N-methylpurine DNA glycosylase with CRISPR systems achieved targeted G-to-T editing with 4.94–12.50% efficiency in rice (Oryza sativa). The combined use of the DNA glycosylase and deaminases enabled co-editing of target guanines with adenines or cytosines.

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来源期刊

Journal of Integrative Plant Biology 生物-生化与分子生物学

CiteScore

18.00

自引率

5.30%

发文量

220

审稿时长

3 months

期刊介绍： Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.