基于外果皮散发的乙醇蒸汽评估 "La France "梨成熟阶段的生物荧光气体成像系统。

IF 8.2 1区 化学 Q1 CHEMISTRY, ANALYTICAL
ACS Sensors Pub Date : 2024-10-25 Epub Date: 2024-06-25 DOI:10.1021/acssensors.4c00642
Kenta Iitani, Kenta Ichikawa, Koji Toma, Takahiro Arakawa, Kohji Mitsubayashi
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引用次数: 0

摘要

水果可以释放乙醇,乙醇是在缺氧贮藏期间通过发酵产生的。我们通过 "La France "梨的外果皮对其释放的气态乙醇的时空变化进行了成像。气体成像系统利用酶将乙醇浓度转化为荧光强度。首先,对酶和辅酶分布的均匀性进行了评估,以验证成像能力。随后,比较了两种表面拟合方法,以准确成像具有双曲面的三维(3D)物体发出的乙醇。梨中乙醇的成像结果表明,乙醇的分布与外果皮上的皮孔有关,据报道,皮孔具有很高的乙醇扩散性。根据该系统的定量分析(辅酶和酶的均匀度分别为 93.2% 和 98.8%;动态范围为 0.01-100 ppm),在缺氧条件下,乙醇浓度随着贮藏时间的延长而增加(从第 1 天到第 10 天为 0.4-5.3 ppm)。该系统可观测水果中乙醇释放的位置、数量和时间模式,是一项有用的农业应用技术。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Biofluorometric Gas-Imaging System for Evaluating the Ripening Stages of "La France" Pear Based on Ethanol Vapor Emitted <i>via</i> the Epicarp.

Biofluorometric Gas-Imaging System for Evaluating the Ripening Stages of "La France" Pear Based on Ethanol Vapor Emitted via the Epicarp.

Fruits can emit ethanol, which is generated through fermentation during hypoxic storage. We imaged spatiotemporal changes in the gaseous ethanol emitted by "La France" pear via its epicarp. The gas-imaging system utilized enzymes to transduce the ethanol concentration into fluorescence intensity. Initially, the uniformity of the enzyme and coenzyme distribution was evaluated to validate the imaging capability. Subsequently, two surface-fitting methods were compared to accurately image ethanol emitted from three-dimensional (3D) objects with a double-curved surface. The imaging results of ethanol emitted from the pear indicated that the distribution of ethanol was related to lenticels, which have been reported to possess high ethanol diffusivity, on the epicarp. As quantified by the system (uniformity of coenzyme and enzymes was 93.2 and 98.8%, respectively; dynamic range was 0.01-100 ppm), ethanol concentration increased with the storage period under hypoxic conditions (0.4-5.3 ppm, from day 1 to 10). The system enables the observation of the location, quantity, and temporal pattern of ethanol release from fruit, which could be a useful technology for agricultural applications.

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来源期刊
ACS Sensors
ACS Sensors Chemical Engineering-Bioengineering
CiteScore
14.50
自引率
3.40%
发文量
372
期刊介绍: ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.
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