黄酮类格桑皮素可保护牙槽骨并限制结扎诱发的小鼠牙周炎的炎症反应

IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Jiwon Seok, Myoung Ok Kim, Sung‐Hyun Kim, Ka‐Young Ryu, Jae‐Young Kim, Heon‐Jin Lee, Yong‐Gun Kim, Youngkyun Lee
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引用次数: 0

摘要

背景在牙周炎中,细菌引起的炎症会破坏牙齿周围的软硬组织。在严重的情况下,破骨细胞数量和活性的增加会诱发牙槽骨的吸收,最终导致牙齿脱落。方法在本研究中,我们利用小鼠骨髓巨噬细胞(BMM)前体的破骨细胞体外培养和体内结扎诱导的小鼠牙周炎模型,证实了格桑吡丁(一种六羟基黄酮)对破骨细胞分化和骨吸收的抑制作用。结果格桑吡丁能在核因子κB配体受体激活剂(RANKL)存在的情况下明显减少小鼠骨髓巨噬细胞前体破骨细胞的分化。在体外,格塞品抑制了 RANKL 下游的关键信号事件,包括活化 T 细胞核因子的自动扩增、细胞质 1、Ca2+ 振荡和活性氧的产生。在小鼠结扎诱导的牙周炎模型中,格桑吡丁能显著减少破骨细胞的生成和牙槽骨的吸收。此外,格桑吡丁还能防止结扎诱导的巨噬细胞和 T 细胞的增加,并减少肿瘤坏死因子-α 和白细胞介素-6 的产生。结论综上所述,这些结果表明格桑吡丁具有抗破骨细胞生成和抗炎作用,表明这种天然化合物有可能用于治疗牙周炎。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Flavonoid gossypetin protects alveolar bone and limits inflammation in ligature‐induced periodontitis in mice
BackgroundBacterial‐induced inflammation instigates the destruction of hard and soft tissues surrounding teeth in periodontitis. In severe cases, the increased number and activity of osteoclasts induces the resorption of alveolar bones, ultimately leading to tooth loss. Because of their diverse chemical structures and bioactivities, natural compounds are often suggested to treat a wide variety of diseases, including inflammatory disorders.MethodsIn the present study, we demonstrated an inhibitory effect of gossypetin, a hexahydroxy flavone, on osteoclast differentiation and bone resorption using in vitro culture of osteoclasts from mouse bone marrow macrophage (BMM) precursors and in vivo model of ligature‐induced periodontitis in mice.ResultsGossypetin significantly reduced the differentiation of osteoclasts from mouse BMM precursors in the presence of the receptor activator of nuclear factor κB ligand (RANKL). In vitro, gossypetin inhibited critical signaling events downstream of RANKL including the auto‐amplification of nuclear factor of activated T‐cells, cytoplasmic 1, Ca2+ oscillations, and the generation of reactive oxygen species. In a mouse ligature‐induced periodontitis model, the administration of gossypetin significantly reduced osteoclastogenesis and alveolar bone resorption. Furthermore, gossypetin prevented the ligature‐induced increase in macrophages and T cells and reduced the production of tumor necrosis factor‐α and interleukin‐6.ConclusionTaken together, these results show anti‐osteoclastogenic and anti‐inflammatory effects of gossypetin, suggesting the potential use of this natural compound in periodontitis.
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来源期刊
Journal of periodontology
Journal of periodontology 医学-牙科与口腔外科
CiteScore
9.10
自引率
7.00%
发文量
290
审稿时长
3-8 weeks
期刊介绍: The Journal of Periodontology publishes articles relevant to the science and practice of periodontics and related areas.
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