{"title":"硫化氢对冷藏番茄果实耐寒性的改善有能量代谢和细胞壁代谢的参与。","authors":"Changxia Li, Junrong Xu, Yunzhi Liu, Xuefang Lu, Shaoxia Li, Jing Cui, Jin Qi, Wenjin Yu","doi":"10.1007/s00299-024-03263-2","DOIUrl":null,"url":null,"abstract":"<p><strong>Key message: </strong>Hydrogen sulfide improved cold resistance of tomato fruits by regulating energy metabolism and delaying cell wall degradation, thereby alleviating the damage of cold storage on fruits. Postharvest cold storage in tomato fruits extended shelf life but caused the appearance of chilling injury (CI), appeared by softness and spots on the surface of the fruits. These changes were linked closely with energy and cell wall metabolisms. Hydrogen sulfide (H<sub>2</sub>S), as the gaseous fresh-keeping regulator, was used in the present study to investigate the effects of H<sub>2</sub>S on energy and cell wall metabolisms in tomato fruits during cold storage. Fruits after harvest were fumigated with different concentrations (0, 0.5, 1, 1.5 mM) of sodium hydrosulfide (NaHS) solution as H<sub>2</sub>S honor for 24 h and stored at 4 °C for 25 days. The results showed that 1 and 1.5 mM NaHS solution fumigation promoted the accumulation of endogenous H<sub>2</sub>S, followed by the increase in <sub>L</sub>-cysteine desulfurase (LCD) and <sub>D</sub>-cysteine desulfurase (DCD) activities in fruits during cold storage. It was also found that 1 and 1.5 mM NaHS treatments improved H<sup>+</sup>-ATPase, Ca<sup>2+</sup>-ATPase, cytochrome C oxidase (CCO), and succinic dehydrogenase (SDH) activities. Moreover, the contents of cellulose and hemicellulose were increased by 1 and 1.5 mM NaHS, following down-regulated activities of cellulase (CL), pectin lyase (PL), α-mannosidase (α-man) and β-Galactosidase (β-Gal) and down-regulated expression of PL1, PL8, MAN4 and MAN7 genes. Thus, H<sub>2</sub>S alleviates CI led by cold storage in tomato fruits via regulating energy and cell wall metabolisms.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":null,"pages":null},"PeriodicalIF":5.3000,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Involvement of energy and cell wall metabolisms in chilling tolerance improved by hydrogen sulfide in cold-stored tomato fruits.\",\"authors\":\"Changxia Li, Junrong Xu, Yunzhi Liu, Xuefang Lu, Shaoxia Li, Jing Cui, Jin Qi, Wenjin Yu\",\"doi\":\"10.1007/s00299-024-03263-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Key message: </strong>Hydrogen sulfide improved cold resistance of tomato fruits by regulating energy metabolism and delaying cell wall degradation, thereby alleviating the damage of cold storage on fruits. Postharvest cold storage in tomato fruits extended shelf life but caused the appearance of chilling injury (CI), appeared by softness and spots on the surface of the fruits. These changes were linked closely with energy and cell wall metabolisms. Hydrogen sulfide (H<sub>2</sub>S), as the gaseous fresh-keeping regulator, was used in the present study to investigate the effects of H<sub>2</sub>S on energy and cell wall metabolisms in tomato fruits during cold storage. Fruits after harvest were fumigated with different concentrations (0, 0.5, 1, 1.5 mM) of sodium hydrosulfide (NaHS) solution as H<sub>2</sub>S honor for 24 h and stored at 4 °C for 25 days. The results showed that 1 and 1.5 mM NaHS solution fumigation promoted the accumulation of endogenous H<sub>2</sub>S, followed by the increase in <sub>L</sub>-cysteine desulfurase (LCD) and <sub>D</sub>-cysteine desulfurase (DCD) activities in fruits during cold storage. It was also found that 1 and 1.5 mM NaHS treatments improved H<sup>+</sup>-ATPase, Ca<sup>2+</sup>-ATPase, cytochrome C oxidase (CCO), and succinic dehydrogenase (SDH) activities. Moreover, the contents of cellulose and hemicellulose were increased by 1 and 1.5 mM NaHS, following down-regulated activities of cellulase (CL), pectin lyase (PL), α-mannosidase (α-man) and β-Galactosidase (β-Gal) and down-regulated expression of PL1, PL8, MAN4 and MAN7 genes. Thus, H<sub>2</sub>S alleviates CI led by cold storage in tomato fruits via regulating energy and cell wall metabolisms.</p>\",\"PeriodicalId\":20204,\"journal\":{\"name\":\"Plant Cell Reports\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2024-06-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Cell Reports\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00299-024-03263-2\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Cell Reports","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00299-024-03263-2","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
关键信息：硫化氢通过调节能量代谢和延缓细胞壁降解来提高番茄果实的抗寒性，从而减轻冷藏对果实的伤害。番茄果实采后冷藏可延长货架期，但会造成冷害（CI），表现为果实表面变软和出现斑点。这些变化与能量和细胞壁代谢密切相关。硫化氢（H2S）是一种气态保鲜调节剂，本研究使用硫化氢来研究冷藏期间硫化氢对番茄果实能量和细胞壁代谢的影响。采收后的果实用不同浓度（0、0.5、1、1.5 mM）的硫氢化钠（NaHS）溶液作为 H2S 名誉熏蒸 24 小时，然后在 4 °C 下贮藏 25 天。结果表明，1 毫摩尔和 1.5 毫摩尔的 NaHS 溶液熏蒸会促进内源 H2S 的积累，随后冷藏期间水果中 L-半胱氨酸脱硫酶（LCD）和 D-半胱氨酸脱硫酶（DCD）的活性也会增加。研究还发现，1 mM 和 1.5 mM NaHS 处理可提高 H+-ATPase、Ca2+-ATPase、细胞色素 C 氧化酶（CCO）和琥珀酸脱氢酶（SDH）的活性。此外，1 mM 和 1.5 mM NaHS 可提高纤维素和半纤维素的含量，但纤维素酶（CL）、果胶裂解酶（PL）、α-甘露糖苷酶（α-man）和β-半乳糖苷酶（β-Gal）的活性下调，PL1、PL8、MAN4 和 MAN7 基因的表达下调。因此，H2S 可通过调节能量和细胞壁代谢缓解番茄果实冷藏导致的 CI。
Involvement of energy and cell wall metabolisms in chilling tolerance improved by hydrogen sulfide in cold-stored tomato fruits.
Key message: Hydrogen sulfide improved cold resistance of tomato fruits by regulating energy metabolism and delaying cell wall degradation, thereby alleviating the damage of cold storage on fruits. Postharvest cold storage in tomato fruits extended shelf life but caused the appearance of chilling injury (CI), appeared by softness and spots on the surface of the fruits. These changes were linked closely with energy and cell wall metabolisms. Hydrogen sulfide (H2S), as the gaseous fresh-keeping regulator, was used in the present study to investigate the effects of H2S on energy and cell wall metabolisms in tomato fruits during cold storage. Fruits after harvest were fumigated with different concentrations (0, 0.5, 1, 1.5 mM) of sodium hydrosulfide (NaHS) solution as H2S honor for 24 h and stored at 4 °C for 25 days. The results showed that 1 and 1.5 mM NaHS solution fumigation promoted the accumulation of endogenous H2S, followed by the increase in L-cysteine desulfurase (LCD) and D-cysteine desulfurase (DCD) activities in fruits during cold storage. It was also found that 1 and 1.5 mM NaHS treatments improved H+-ATPase, Ca2+-ATPase, cytochrome C oxidase (CCO), and succinic dehydrogenase (SDH) activities. Moreover, the contents of cellulose and hemicellulose were increased by 1 and 1.5 mM NaHS, following down-regulated activities of cellulase (CL), pectin lyase (PL), α-mannosidase (α-man) and β-Galactosidase (β-Gal) and down-regulated expression of PL1, PL8, MAN4 and MAN7 genes. Thus, H2S alleviates CI led by cold storage in tomato fruits via regulating energy and cell wall metabolisms.
期刊介绍：
Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as:
- genomics and genetics
- metabolism
- cell biology
- abiotic and biotic stress
- phytopathology
- gene transfer and expression
- molecular pharming
- systems biology
- nanobiotechnology
- genome editing
- phenomics and synthetic biology
The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
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