在阿根廷科连特斯的水豚(Hydrochoerus hydrochaeris)体内分子检测到边缘型阿那普拉原虫。

IF 1.1 4区 农林科学 Q3 VETERINARY SCIENCES
Diana Arnica, María M Orozco, Iara Figini, Paula Blanco, Cecilia Li Puma, Marisa D Farber, Eliana C Guillemi
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引用次数: 0

摘要

监测野生动物的健康状况对于了解全球疾病模式至关重要,尤其是当病媒传染病的地理范围因环境变化而扩大,宿主也随之扩大时更是如此。边缘疟原虫(Anaplasma marginale)主要影响牛群,具有经济影响,已在多种宿主中发现,但对其在毛冠鱼(Hydrochoerus hydrochaeris)中的存在缺乏全面了解,而毛冠鱼在蜱媒病原体传播中具有影响力。从 2015 年到 2022 年,对阿根廷东北部两个不同地区的 14 只水豚进行了调查。通过扩增特定基因 msp5 和 msp1β,14 只毛豚鼠中的 1 只(7%)确认了 A. marginale 的存在。此外,通过定量聚合酶链式反应(PCR),在毛豚鼠的血液样本中检测到了毛豚鼠DNA,周期阈值为30.81(每次反应800个拷贝)。对 msp1α 基因片段的扩增发现了三种不同大小的 PCR 产物,这表明在水豚宿主体内至少存在三种共同感染的 A. marginale 变种。这项研究表明,在阿根廷伊比利亚湿地,水豚是边缘疟原虫的野生宿主,有可能影响家养物种和野生物种的感染动态。这一发现凸显了对水豚在疾病动态中的作用进行深入研究的必要性,这对了解野生动物健康和疾病传播至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular Detection of Anaplasma marginale in Capybara (Hydrochoerus hydrochaeris) from Corrientes, Argentina.

Monitoring wildlife health is essential for understanding global disease patterns, particularly as vector-borne infections extend the geographic ranges and thereby hosts due to environmental shifts. Anaplasma marginale, primarily impacting cattle, has economic implications and has been found in diverse hosts, yet its presence in capybaras (Hydrochoerus hydrochaeris), influential in tick-borne pathogen spread, lacks comprehensive understanding. From 2015 to 2022, 14 capybaras were surveyed across two different areas of northeastern Argentina. In 1 of 14 (7%) capybaras, the presence of A. marginale was confirmed through the amplification of specific genes, msp5 and msp1β. In addition, A. marginale DNA was detected in the capybara's blood sample through quantitative PCR, with a cycle threshold value of 30.81 (800 copies per reaction). Amplification of a fragment of the msp1α gene revealed PCR products of three different sizes, suggesting the presence of at least three coinfecting A. marginale variants in the capybara host. This study suggests that capybaras are wild hosts for A. marginale in the Ibera Wetlands in Argentina, potentially influencing the infection dynamics of both domestic and wild species. This finding highlights the necessity for thorough studies on the role of capybaras in disease dynamics, crucial for understanding wildlife health and the spread of disease.

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来源期刊
Journal of Wildlife Diseases
Journal of Wildlife Diseases 农林科学-兽医学
CiteScore
2.70
自引率
0.00%
发文量
213
审稿时长
6-16 weeks
期刊介绍: The JWD publishes reports of wildlife disease investigations, research papers, brief research notes, case and epizootic reports, review articles, and book reviews. The JWD publishes the results of original research and observations dealing with all aspects of infectious, parasitic, toxic, nutritional, physiologic, developmental and neoplastic diseases, environmental contamination, and other factors impinging on the health and survival of free-living or occasionally captive populations of wild animals, including fish, amphibians, reptiles, birds, and mammals. Papers on zoonoses involving wildlife and on chemical immobilization of wild animals are also published. Manuscripts dealing with surveys and case reports may be published in the Journal provided that they contain significant new information or have significance for better understanding health and disease in wild populations. Authors are encouraged to address the wildlife management implications of their studies, where appropriate.
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